Patents by Inventor John Crowley

John Crowley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • High Concentration Alpha-Glucosidase Compositions For The Treatment Of Pompe Disease
    Publication number: 20260083829
    Abstract: The present application provides for compositions comprising high concentrations of acid ?-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Application
    Filed: September 22, 2025
    Publication date: March 26, 2026
    Applicant: Amicus Therapeutics, Inc.
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • METHODS OF CHARACTERIZING AND PURIFYING VEGF RECEPTOR FUSION PROTEIN
    Publication number: 20260001935
    Abstract: Anti-VEGF proteins including the VEGF trap protein aflibercept can be produced to have a low level of an aspartyl protease (e.g., a low level of cathepsin D), such as less than 1 ppm of the aspartyl protease.
    Type: Application
    Filed: September 5, 2025
    Publication date: January 1, 2026
    Inventors: Paul CONROY, John CROWLEY, Marguerita McCARTHY, Kathleen O'DWYER, Shunhai WANG, Sisi ZHANG, Yuetian YAN, Tyler GREER, Siobhan HOGAN, Xiaojing ZHENG, Hui XIAO, Ning LI, Lili GUO, Yuan MAO, Prerana MANTRI, Evan KOUFOS, Kathir MUTHUSAMY, Nisha PALACKAL, Erica PYLES
  • High concentration alpha-glucosidase compositions for the treatment of Pompe disease
    Patent number: 12419937
    Abstract: The present application provides for compositions comprising high concentrations of acid ?-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Grant
    Filed: March 21, 2022
    Date of Patent: September 23, 2025
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • MANUFACTURING PROCESS FOR HIGH TITER ANTIBODY
    Publication number: 20240010737
    Abstract: The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.
    Type: Application
    Filed: February 28, 2023
    Publication date: January 11, 2024
    Inventors: John Mattila, Xiaolin Tang, Hanne Bak, Shawn M. Lawrence, Amy S. Johnson, Meghan Casey, Michelle LaFond, Andrew Tustian, Philip Mellors, John Hourihan, John Crowley, Laura Callinan, Shadia Abike Oshodi, Ashley Witmer, Daniel Corbett, James Reilly, Ankit Vartak, Mark Chiboroski, Alessandra Starling, Robert Stairs, Hai-Yuan Goh, Liam Nicholl, Aishling Conlon
  • MANUFACTURING PROCESS FOR HIGH TITER ANTIBODY
    Publication number: 20230348532
    Abstract: The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.
    Type: Application
    Filed: February 28, 2023
    Publication date: November 2, 2023
    Inventors: John Mattila, Xiaolin Tang, Hanne Bak, Shawn M. Lawrence, Amy S. Johnson, Meghan Casey, Michelle LaFond, Andrew Tustian, Philip Mellors, John Hourihan, John Crowley, Laura Callinan, Shadia Abike Oshodi, Ashley Witmer, Daniel Corbett, James Reilly, Ankit Vartak, Mark Chiboroski, Alessandra Starling, Robert Stairs, Hai-Yuan Goh, Liam Nicholl, Aishling Conlon
  • CELL CULTURE METHODS FOR ANTIBODY PRODUCTION
    Publication number: 20230332201
    Abstract: The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.
    Type: Application
    Filed: February 28, 2023
    Publication date: October 19, 2023
    Inventors: John Mattila, Xiaolin Tang, Hanne Bak, Shawn M. Lawrence, Amy S. Johnson, Meghan Casey, Michelle LaFond, Andrew Tustian, Philip Mellors, John Hourihan, John Crowley, Laura Callinan, Shadia Abike Oshodi, Ashley Witmer, Daniel Corbett, James Reilly, Ankit Vartak, Mark Chiboroski, Alessandra Starling, Robert Stairs, Hai-Yuan Goh, Liam Nicholl, Aishling Conlon
  • MANUFACTURING PROCESS FOR HIGH TITER ANTIBODY
    Publication number: 20230331776
    Abstract: The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.
    Type: Application
    Filed: February 28, 2023
    Publication date: October 19, 2023
    Inventors: John Mattila, Xiaolin Tang, Hanne Bak, Shawn M. Lawrence, Amy S. Johnson, Meghan Casey, Michelle LaFond, Andrew Tustian, Philip Mellors, John Hourihan, John Crowley, Laura Callinan, Shadia Abike Oshodi, Ashley Witmer, Daniel Corbett, James Reilly, Ankit Vartak, Mark Chiboroski, Alessandra Starling, Robert Stairs, Hai-Yuan Goh, Liam Nicholl, Aishling Conlon
  • BIOREACTOR FOR ANTIBODY PRODUCTION
    Publication number: 20230332084
    Abstract: The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.
    Type: Application
    Filed: February 28, 2023
    Publication date: October 19, 2023
    Inventors: John Mattila, Xiaolin Tang, Hanne Bak, Shawn M. Lawrence, Amy Johnson, Meghan Casey, Michelle LaFond, Andrew Tustian, Philip Mellors, John Hourihan, John Crowley, Laura Callinan, Shadia Abike Oshodi, Ashley Witmer, Daniel Corbett, James Reilly, Ankit Vartak, Mark Chiboroski, Alessandra Starling, Robert Stairs, Hai-Yuan Goh, Liam Nicholl, Aishling Conlon
  • CELL CULTURE METHODS FOR ANTIBODY PRODUCTION
    Publication number: 20230287043
    Abstract: The present invention pertains to methods for manufacturing high titer antibody products. In particular, the invention pertains, in part, to improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Additionally, the present invention further pertains to chromatographic procedures employed to successfully isolate the antibody product subject of the present disclosure.
    Type: Application
    Filed: February 28, 2023
    Publication date: September 14, 2023
    Inventors: John Mattila, Xiaolin Tang, Hanne Bak, Shawn M. Lawrence, Amy S. Johnson, Meghan Casey, Michelle LaFond, Andrew Tustian, Philip Mellors, John Hourihan, John Crowley, Laura Callinan, Shadia Abike Oshodi, Ashley Witmer, Daniel Corbett, James Reilly, Ankit Vartak, Mark Chiboroski, Alessandra Starling, Robert Stairs, Hai-Yuan Goh, Liam Nicholl, Aishling Conlon
  • METHODS FOR QUANTIFYING THE IMPACT OF SHEAR STRESS ON MAMMALIAN CELL LINES
    Publication number: 20230103671
    Abstract: Methods for characterizing mechanical properties of cells at different stress levels. The disclosed inventions can determine the impact of shear stress on cells in bioproduction processes.
    Type: Application
    Filed: September 30, 2022
    Publication date: April 6, 2023
    Inventors: Ian O'Shea, John Crowley, Martin Power, Alan Ronan
  • METHODS OF CONTROLLING ANTIBODY HETEROGENEITY
    Publication number: 20230090912
    Abstract: The present inventions provide methods to control the heterogeneity of Fc-containing proteins, such as antibodies produced in cell culture, particularly mammalian cell culture by controlling culture pCO2, as well as products produced by these methods. Among other things, the inventions provide for lowering the percentage of acidic charge variants in antibody products. Proteins that comprise Fc moieties include but are not limited to Fc-containing proteins, such as antibodies and antibody derivatives, and fragments of both.
    Type: Application
    Filed: September 20, 2022
    Publication date: March 23, 2023
    Inventors: Philip Mellors, John Hourihan, John Crowley
  • HIGH CONCENTRATION ALPHA-GLUCOSIDASE COMPOSITIONS FOR THE TREATMENT OF POMPE DISEASE
    Publication number: 20220347272
    Abstract: The present application provides for compositions comprising high concentrations of acid ?-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Application
    Filed: March 21, 2022
    Publication date: November 3, 2022
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • High concentration alpha-glucosidase compositions for the treatment of Pompe disease
    Patent number: 11278599
    Abstract: The present application provides for compositions comprising high concentrations of acid a-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Grant
    Filed: September 24, 2019
    Date of Patent: March 22, 2022
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • Thermocouple assembly
    Patent number: 11009402
    Abstract: Disclosed is a thermocouple assembly having a resilient mount; a buffer having a first side and a second side, with the buffer first side connecting with the resilient mount; an insulator having a first side and a second side, with the insulator first side connecting with the buffer second side; and a thermocouple having a first side and a second side, the thermocouple first side connecting with the insulator second side, and the thermocouple second side being configured to be in thermal contact with an object to be measured.
    Type: Grant
    Filed: May 16, 2018
    Date of Patent: May 18, 2021
    Assignee: U.S. Department of Energy
    Inventors: Nelson Schwarting, Brian J. Lashway, John Crowley, Don Rappold
  • HIGH CONCENTRATION ALPHA-GLUCOSIDASE COMPOSITIONS FOR THE TREATMENT OF POMPE DISEASE
    Publication number: 20200188494
    Abstract: The present application provides for compositions comprising high concentrations of acid a-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Application
    Filed: September 24, 2019
    Publication date: June 18, 2020
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • High concentration alpha-glucosidase compositions for the treatment of pompe disease
    Patent number: 10512677
    Abstract: The present application provides for compositions comprising high concentrations of acid a-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: December 24, 2019
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • THERMOCOUPLE ASSEMBLY
    Publication number: 20190170590
    Abstract: Disclosed is a thermocouple assembly having a resilient mount; a buffer having a first side and a second side, with the buffer first side connecting with the resilient mount; an insulator having a first side and a second side, with the insulator first side connecting with the buffer second side; and a thermocouple having a first side and a second side, the thermocouple first side connecting with the insulator second side, and the thermocouple second side being configured to be in thermal contact with an object to be measured.
    Type: Application
    Filed: May 16, 2018
    Publication date: June 6, 2019
    Applicant: United States Department of Energy
    Inventors: Nelson Schwarting, Brian J. Lashway, John Crowley, Don Rappold
  • HIGH CONCENTRATION ALPHA-GLUCOSIDASE COMPOSITIONS FOR THE TREATMENT OF POMPE DISEASE
    Publication number: 20180360928
    Abstract: The present application provides for compositions comprising high concentrations of acid a-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Application
    Filed: June 22, 2018
    Publication date: December 20, 2018
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • High concentration alpha-glucosidase compositions for the treatment of Pompe disease
    Patent number: 10046033
    Abstract: The present application provides for compositions comprising high concentrations of acid a-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: August 14, 2018
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan
  • High Concentration Alpha-Glucosidase Compositions For The Treatment Of Pompe Disease
    Publication number: 20170056483
    Abstract: The present application provides for compositions comprising high concentrations of acid ?-glucosidase in combination with an active site-specific chaperone for the acid ?-glucosidase, and methods for treating Pompe disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an acid ?-glucosidase enzyme formulation.
    Type: Application
    Filed: June 24, 2016
    Publication date: March 2, 2017
    Inventors: Kenneth Valenzano, John Crowley, Richie Khanna, John Flanagan