Abstract: A synthetic, nuclear-encoded ND4 gene was linked to a mitochondrial targeting sequence and a FLAG epitope tag. This fusion construct was inserted into a rAAV vector. The ND4 fusion protein was expressed and imported into the mitochondria of cells harboring a mitochondrial DNA mutation (G11778A), where it restored cellular respiration.

Abstract: The invention is the novel use of dispersion compensation in a long haul wavelength division multiplexed high capacity optical transport system which has very many channels packed extremely closely together, in order to greatly reduce the deleterious effects of four-wave mixing. Four-wave mixing is an exchange of energy between nominally independent channels, arising from the fundamental fibre non-linearity, which has the effect of degrading transmission quality. Conventional systems make use of fibre dispersion compensating modules to overcome the effects of fibre dispersion. In such systems, it has been discovered that the exact distribution of fibre dispersion along the optical link (the ‘dispersion map’) strongly influences the degree of four-wave mixing, and hence the degradation in transmission quality.