Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract: A heated hand grip that is adhered onto the grip section of the bow or the handle section of the fishing rod includes a flexible substrate, a malleable heating element, and a power port. The flexible substrate includes a conductive layer and an insulating layer, wherein the conductive layer is superimposed onto the insulating layer. The malleable heating element is integrated in between the conductive layer and the insulating layer. The power port is externally connected onto the flexible substrate as the malleable heating element is electrically connected to the power port. When the malleable heating element is powered through the power port, the malleable heating element is able to provide warmth to the conductive layer which in return warms the exposed hands of the bow hunters and anglers.

Abstract: Disclosed herein are light emitting diode devices having one or more high reflectivity wide bonding pad electrodes and methods of fabricating thereof.

Abstract: Disclosed herein are light emitting diode devices having one or more high reflectivity mesa sidewall electrodes and methods of fabricating thereof.

Abstract: One variation of a method for managing parking within a parking structure includes: after detecting a license plate number on a vehicle in an image captured by an entry camera unit arranged near an entry of the parking structure, labeling the license plate as unverified in a data log associated with the parking structure; in response to absence of validation of the license plate within a grace period after entry of the vehicle, relabeling the license plate as in-violation in the data log; in response to detecting the license plate in an image captured by a mobile enforcement unit moving through the parking structure, querying the data log for a status of the license plate; and, in response to the license plate labeled as in-violation in the data log, serving a prompt to an enforcement personnel to issue a citation to a vehicle carrying the license plate.

Abstract: An engineered wafer includes a plurality of mesa structures that includes a first mesa structure and a second mesa structure. The first mesa structure includes a first porous layer of a first semiconductor material having a first lattice constant, and a first layer of a second semiconductor material on the first porous layer. The first porous layer is characterized by a first porosity. The second semiconductor material is characterized by a second lattice constant greater than the first lattice constant. The second mesa structure includes a second porous layer of the first semiconductor material, and a second layer of the second semiconductor material on the second porous layer. The second porous layer is characterized by a second porosity different from the first porosity. Active regions grown on the first and second layers of the second semiconductor material are configured to emit light of different colors.

Abstract: A light emitting diode (LED) device includes a substrate and a plurality of mesa structures. Each mesa structure includes a layer of a first semiconductor material, a porous layer of the first semiconductor material on the layer of the first semiconductor material, and a layer of a second semiconductor material on the porous layer. The porous layer is characterized by an areal porosity ?15%. The second semiconductor material is characterized by a lattice constant greater than a lattice constant of the first semiconductor material. Each mesa structure also includes an active region on the layer of the second semiconductor material and configured to emit red light, a p-contact layer on the active region, a dielectric layer on sidewalls of the p-contact layer and the active region, and an n-contact layer in physical contact with at least a portion of sidewalls of the layer of the second semiconductor material.

Abstract: One variation of a method for managing parking within a parking structure includes: after detecting a license plate number on a vehicle in an image captured by an entry camera unit arranged near an entry of the parking structure, labeling the license plate as unverified in a data log associated with the parking structure; in response to absence of validation of the license plate within a grace period after entry of the vehicle, relabeling the license plate as in-violation in the data log; in response to detecting the license plate in an image captured by a mobile enforcement unit moving through the parking structure, querying the data log for a status of the license plate; and, in response to the license plate labeled as in-violation in the data log, serving a prompt to an enforcement personnel to issue a citation to a vehicle carrying the license plate.

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract: One variation of a method can be implemented by a computer system (e.g., a computer network, a remote server, a local parking enforcement kiosk) in cooperation with a set of sensor units installed below restricted parking spaces (e.g., loading zones, freight parking spaces) and vehicle transmitters installed in authorized vehicles (e.g., delivery vehicles, commercial vehicles) to automatically enforce parking rules in restricted parking spaces, such as including: parking time limits; grace periods for unauthorized vehicles; billing rates for authorized vehicles; and issuance of citations for unauthorized vehicles occupying such parking spaces beyond the grace period.

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.

Abstract: Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

Abstract: One variation of a method for managing parking within a parking structure includes: after detecting a license plate number on a vehicle in an image captured by an entry camera unit arranged near an entry of the parking structure, labeling the license plate as unverified in a data log associated with the parking structure; in response to absence of validation of the license plate within a grace period after entry of the vehicle, relabeling the license plate as in-violation in the data log; in response to detecting the license plate in an image captured by a mobile enforcement unit moving through the parking structure, querying the data log for a status of the license plate; and, in response to the license plate labeled as in-violation in the data log, serving a prompt to an enforcement personnel to issue a citation to a vehicle carrying the license plate.

Abstract: Methods of producing substrates having selected active chemical regions by employing elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate. The methods may include optical, chemical and/or mechanical processes for the deposition, removal, activation and/or deactivation of chemical groups in selected regions of the substrate to provide selective active regions of the substrate.

Abstract: The different advantageous embodiments further provide a system for hierarchical mission management comprising a number of mission planners, a data processing system, and a communication system. The number of mission planners are associated with a number of agent groups. The data processing system is configured to execute the number of mission planners. The communication system is configured to provide communication between the number of mission planners.

Abstract: The different advantageous embodiments further provide a system for hierarchical mission management comprising a number of mission planners, a data processing system, and a communication system. The number of mission planners are associated with a number of agent groups. The data processing system is configured to execute the number of mission planners. The communication system is configured to provide communication between the number of mission planners.

Abstract: Protected fluorescent reagent compounds and their methods of synthesis are provided. The compounds are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The compounds contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements.