Abstract: A seat assembly includes a seat portion having a frame assembly. A routing mechanism includes a plate member that is operably coupled to the frame assembly. The plate member includes a first portion having first and second raised portions spaced-apart from one another to define a channel therebetween. The routing mechanism further includes a connection assembly having upper and lower tabs spaced-apart from one another with a routing column assembly interconnecting the upper and lower tabs. A tether member includes a body portion that is routed through the routing mechanism, in that the body portion of the tether member is received in the channel of the routing mechanism and is wrapped around the routing column assembly and directed in forward direction.

Abstract: Systems and methods taught herein advantageously provide extended dynamic range capabilities to detect low intensity and high intensity emitted or scattered light from particles at high speeds with high sensitivity. Independently controlled first and second optical detector elements that handle light intensities in different dynamic ranges, large overall dynamic range is created. Signals from the detector elements can be combined to create a single combined signal that has excellent sensitivity over a large dynamic range. The detector systems and methods taught herein are particularly advantageous in particle processing where the population of particles can emit or scatter light over a large range of intensity values.

Abstract: A method, system and apparatus for anti-sorting particles is disclosed. In an example embodiment, particles move in a fluid along a microfluidic channel. A measurement device determines which particles are selected or desired particles by measuring a desired characteristic of the particles and outputting an associated signal. The energy source continuously imparts a force on unselected or undesired particles in a deflection region of the microfluidic channel to remove the unselected or undesired particles from the stream of particles. Based upon the signal outputted by the measurement device, the energy source is controlled to reduce or eliminate the force on selected particles such that the selected particles flow on a natural or expected flow path to a collection area such as an output channel. By avoiding application of force to desired particles, the anti-sorting systems and methods can improve viability and other characteristics of the desired particles.

Abstract: A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbilng a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.

Abstract: In a tracking device system, a networked smart device is enabled to cause execution of an executable function if a trigger condition of a conditional rule is satisfied. The conditional rules are customizable on a user interface of the smart device. The trigger condition may be any information received in a signal from a tracking device, for example, sensor data, location, a time, a date, a button press, a proximity, and more generally a trigger condition can be any condition that has a truth value by which an IF/THEN conditional rule can be evaluated and either is satisfied or is not. Executable functions for use in conditional rules may be selected from functions of a smart device, but also may include any function of a remote machine, network or system. Bluetooth radiotag devices may also include a cellular radio modem for global networking. Network systems may include software for tracking groups of radiotags and for autoprovisioning location and tracking services.

Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles).

Abstract: Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

Abstract: XCB dual-radio devices as finders, locators, scanners, sensors, and radio topology reporters for pet location management services. Finding, tracking, scanning, locating and proximity monitoring are provided as complementary services supplemented by a Bluetooth Proximity Locator Services Toolkit and a Cellular Remote Locator Services Toolkit. The radiotag devices may also include a user interface with exterior “homing” switch accessible to a user. The switch may activate an “on demand” location fix and communication package useful in pet location services and recovery. For example, smart systems enable alerts, messaging, maps and mixed media support as community services for pet location and tracking.

Abstract: A method of sorting cells including magnetically coupling a nozzle assembly to a flow cytometer in a predetermined orientation to form a fluid stream having a sheath fluid and a sample fluid. Particles within the sample fluid are oriented and then interrogated at an inspection zone to produces signals representative of emitted or reflected electromagnetic radiation. The electromagnetic radiation is analyzed to classify the cells and the cells are sorted according to their classification.

Abstract: Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

Abstract: A microfluidic chip having a micro channel for processing a sample is provided. The micro channel may focus the sample by using focusing fluid and a core stream forming geometry. The core stream forming geometry may include a lateral fluid focusing component and one or more vertical fluid focusing components. A microfluidic chip may include a plurality micro channels operating in parallel on a microfluidic chip.

Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.

Abstract: A method, system and apparatus for anti-sorting particles is disclosed. In an example embodiment, particles move in a fluid along a microfluidic channel. A measurement device determines which particles are selected or desired particles by measuring a desired characteristic of the particles and outputting an associated signal. The energy source continuously imparts a force on unselected or undesired particles in a deflection region of the microfluidic channel to remove the unselected or undesired particles from the stream of particles. Based upon the signal outputted by the measurement device, the energy source is controlled to reduce or eliminate the force on selected particles such that the selected particles flow on a natural or expected flow path to a collection area such as an output channel. By avoiding application of force to desired particles, the anti-sorting systems and methods can improve viability and other characteristics of the desired particles.

Abstract: A microfluidic chip having a micro channel for processing a sample is provided. The micro channel may focus the sample by using focusing fluid and a core stream forming geometry. The core stream forming geometry may include a lateral fluid focusing component and one or more vertical fluid focusing components. A microfluidic chip may include a plurality micro channels operating in parallel on a microfluidic chip.

Abstract: XCB poly-radio devices as finders, locators, scanners, sensors, and radio topology reporters. XCB devices function as “smart objects” in the TOT and combine transceivers operating in the telecommunications and the low power ISM radio spectra, with default power management setting overrides under local control of a Bluetooth modem controller, and can be authenticated on a variety of local area networks (LANs), personal area networks (PANs), piconets and cellular networks. Finding, wayfinding, tracking, scanning, locating and proximity monitoring are provided as complementary services supplemented by a Bluetooth Proximity Locator Services Toolkit, Cellular Remote Locator Services Toolkit, and a “Tap-2-Connect” community-supported on-demand lost-and-found data or voice link between an owner and a passerby who finds a lost smart object.

Abstract: The present disclosure relates to optical crosstalk reduction in particle processing (e.g., cytometry including flow cytometry using microfluidic based sorters, drop formation based sorters, and/or cell purification) systems and methods in order to improve performance. More particularly, the present disclosure relates to assemblies, systems and methods for minimizing optical crosstalk during the analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting, monitoring and/or enriching) of particles (e.g., cells, microscopic particles, etc.). The exemplary systems and methods for crosstalk reduction in particle processing systems (e.g., cell purification systems) may be particularly useful in the area of cellular medicine or the like. The systems and methods may be modular and used singly or in combination to optimize cell purification based on the crosstalk environment and specific requirements of the operator and/or system.

Abstract: XCB poly-radio devices as finders, locators, scanners, sensors, and radio topology reporters. XCB devices function as “smart objects” in the TOT and combine transceivers operating in the telecommunications and the low power ISM radio spectra, with default power management setting overrides under local control of a Bluetooth modem controller, and can be authenticated on a variety of local area networks (LANs), personal area networks (PANs), piconets and cellular networks. Finding, wayfinding, tracking, scanning, locating and proximity monitoring are provided as complementary services supplemented by a Bluetooth Proximity Locator Services Toolkit, Cellular Remote Locator Services Toolkit, and a “Tap-2-Connect” community-supported on-demand lost-and-found data or voice link between an owner and a passerby who finds a lost smart object.

Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.