Abstract: XCB poly-radio devices as finders, locators, scanners, sensors, and radio topology reporters. XCB devices function as “smart objects” in the TOT and combine transceivers operating in the telecommunications and the low power ISM radio spectra, with default power management setting overrides under local control of a Bluetooth modem controller, and can be authenticated on a variety of local area networks (LANs), personal area networks (PANs), piconets and cellular networks. Finding, wayfinding, tracking, scanning, locating and proximity monitoring are provided as complementary services supplemented by a Bluetooth Proximity Locator Services Toolkit, Cellular Remote Locator Services Toolkit, and a “Tap-2-Connect” community-supported on-demand lost-and-found data or voice link between an owner and a passerby who finds a lost smart object.

Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.

Abstract: XCB poly-radio devices as finders, locators, scanners, sensors, and radio topology reporters. XCB devices function as “smart objects” in the TOT and combine transceivers operating in the telecommunications and the low power ISM radio spectra, with default power management setting overrides under local control of a Bluetooth modem controller, and can be authenticated on a variety of local area networks (LANs), personal area networks (PANs), piconets and cellular networks. Finding, wayfinding, tracking, scanning, locating and proximity monitoring are provided as complementary services supplemented by a Bluetooth Proximity Locator Services Toolkit, Cellular Remote Locator Services Toolkit, and a “Tap-2-Connect” community-supported on-demand lost-and-found data or voice link between an owner and a passerby who finds a lost smart object.

Abstract: The present disclosure provides a method for chemoselective modification of a target molecule. A subject method includes contacting a target molecule comprising a thiol moiety with a biomolecule comprising a reactive moiety, wherein the reactive moiety is generated by reaction of a biomolecule comprising a phenol moiety or a catechol with an enzyme capable of oxidizing the phenol or the catechol moiety. The contacting is carried out under conditions sufficient for conjugation of the target molecule to the biomolecule, thereby producing a modified target molecule. The present disclosure provides compositions comprising a subject target molecule comprising a thiol moiety, and a biomolecule comprising a phenol moiety or a catechol moiety. The present disclosure provides kits for carrying out a subject method. The present disclosure also provides modified target molecules and methods for using same.

Abstract: The present disclosure provides improved optical systems for particle processing (e.g., cytometry including microfluidic based sorters, drop sorters, and/or cell purification) systems and methods. More particularly, the present disclosure provides advantageous micro-lens array optical detection assemblies for particle (e.g., cells, microscopic particles, etc.) processing systems and methods (e.g., for analyzing, sorting, processing, purifying, measuring, isolating, detecting, monitoring and/or enriching particles.

Abstract: In a tracking device system, a networked smart device is enabled to cause execution of an executable function if a trigger condition of a conditional rule is satisfied. The conditional rules are customizable on a user interface of the smart device. The trigger condition may be any information received in a signal from a tracking device, for example, sensor data, location, a time, a date, a button press, a proximity, and more generally a trigger condition can be any condition that has a truth value by which an IF/THEN conditional rule can be evaluated and either is satisfied or is not. Executable functions for use in conditional rules may be selected from functions of a smart device, but also may include any function of a remote machine, network or system. Bluetooth radiotag devices may also include a cellular radio modem for global networking. Network systems may include software for tracking groups of radiotags and for autoprovisioning location and tracking services.

Abstract: XCB poly-radio devices as finders, locators, scanners, sensors, and radio topology reporters. XCB devices function as “smart objects” in the TOT and combine transceivers operating in the telecommunications and the low power ISM radio spectra, with default power management setting overrides under local control of a Bluetooth modem controller, and can be authenticated on a variety of local area networks (LANs), personal area networks (PANs), piconets and cellular networks. Finding, wayfinding, tracking, scanning, locating and proximity monitoring are provided as complementary services supplemented by a Bluetooth Proximity Locator Services Toolkit, Cellular Remote Locator Services Toolkit, and a “Tap-2-Connect” community-supported on-demand lost-and-found data or voice link between an owner and a passerby who finds a lost smart object.

Abstract: A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

Abstract: Pistols have a body having a grip, the grip defining a magazine passage, the body having a trigger guard defining a trigger space, the body having an upper surface, the body having a first mounting facility, an upper frame assembly removably connected to the body in a connected position, the upper frame assembly including a trigger element extending into the trigger space when in the connected position, a protrusion extending upward from the upper surface of the body, the protrusion having a forward-facing first bearing surface, and the upper frame assembly having a rear-facing second bearing surface adapted to abut the first bearing surface when in the connected position to transmit recoil forces from the upper frame assembly to the body. The upper frame assembly may have a slide rail. The upper frame assembly may define a horizontal slide plane.

Abstract: A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

Abstract: Pistols have a body having a grip, the grip defining a magazine passage, the body having a trigger guard defining a trigger space, the body having an upper surface, the body having a first mounting facility, an upper frame assembly removably connected to the body in a connected position, the upper frame assembly including a trigger element extending into the trigger space when in the connected position, a protrusion extending upward from the upper surface of the body, the protrusion having a forward-facing first bearing surface, and the upper frame assembly having a rear-facing second bearing surface adapted to abut the first bearing surface when in the connected position to transmit recoil forces from the upper frame assembly to the body. The upper frame assembly may have a slide rail. The upper frame assembly may define a horizontal slide plane.

Abstract: Systems and methods taught herein advantageously provide extended dynamic range capabilities to detect low intensity and high intensity emitted or scattered light from particles at high speeds with high sensitivity. Independently controlled first and second optical detector elements that handle light intensities in different dynamic ranges, large overall dynamic range is created. Signals from the detector elements can be combined to create a single combined signal that has excellent sensitivity over a large dynamic range. The detector systems and methods taught herein are particularly advantageous in particle processing where the population of particles can emit or scatter light over a large range of intensity values.

Abstract: Systems and methods taught herein enable simultaneous forward and side detection of light originating within a microfluidic channel disposed in a substrate. At least a portion of the microfluidic channel is located in the substrate relative to a first side surface of the substrate to enable simultaneous detection paths with respect to extinction (i.e., 0°) and side detection (i.e., 90°). The location of the microfluidic channel as taught herein enables a maximal half-angle for a ray of light passing from a center of the portion of the microfluidic channel through the first side surface to be in a range from 25 to 90 degrees in some embodiments. By placing at least the portion of the microfluidic channel proximate to the side surface of the substrate, a significantly greater proportion of light emitted or scattered from a particle within the microfluidic channel can be collected and imaged on a detector as compared to conventional particle processing chips.

Abstract: Exemplary embodiments are directed to sensor apparatuses for attachment to an animal that include a housing and a sensor assembly. The housing can be attachable to the animal and includes an internal cavity formed therein. The sensor assembly can be disposed within the internal cavity of the housing. The sensor assembly includes a force sensor and an accelerometer arranged to detect force data and accelerometer data representative of a physiological state of the animal. Exemplary embodiments are also directed to method and sensor systems for detecting a physiological state of an animal.

Abstract: This relates to acoustic microfluidic systems that can generate emulsions/droplets or encapsulate particles of interest (including mammalian cells, bacteria cells, or other cells) into droplets upon detection of the particles of interest flowing in a stream of particles. The systems operate on the detect/decide/deflect principle wherein the deflection step, in a single operation, not only deflects particles of interest from a stream of particles but also encapsulates the particles of interest in an emulsion droplet. The microfluidic systems have an abrupt transition in the channel geometry from a shorter channel to a taller channel (i.e., in the shape of a ‘step’) to break the stream of the dispersed phase into a droplet upon acoustic actuation. When there is no acoustic wave present, no droplets/emulsions are generated and the stream of particles proceeds uninterrupted.

Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.

Abstract: Microfluidic flow-through elements and methods for forming and using the same, particularly, low cost, easily sterilized, disposable microfluidic flow-through elements may include an orifice region suitable, for example, for fluid jet formation (such as in a droplet sorter or flow cell) or sample injection or hydrodynamic focusing (such as in a non-droplet flow cytometer). Laser drilling, for example laser ablation, may be used to form an orifice region extending through an orifice wall section of a base substrate. The base substrate may be unitarily-formed by injection molding a polymeric material. The orifice region may be advantageously configured to form a predetermined geometry by controlling the characteristics of the ablating beam.

Abstract: Pistols have a body having a grip, the grip defining an elongated magazine passage adapted to receive a magazine and defining a magazine axis, the grip having opposed left and right side walls and a front portion and an opposed rear portion, the body having an upper portion having a first linear interface element aligned with an attachment axis offset from the magazine axis, and a removable backstrap element adapted to overlay the rear portion of the grip and having a second linear interface element adapted to removably connect to the first linear interface element. The pistol frame may include slide rails defining a slide plane, and the first linear interface element may be parallel to the slide plane. The first linear interface element and the second linear interface element may comprise a sliding interface. The sliding interface may be a dovetail feature.

Abstract: Pistols have a body having a grip, the grip defining a magazine passage adapted to receive a magazine, the grip having opposed left and right side walls and a front portion and an opposed rear portion, the front portion of the grip including a first front strap attachment facility, a front strap element having a second front strap attachment facility adapted to removably connect to the first front strap attachment facility, and the front portion of the grip defining a front path and the front strap element being an elongated element movable along the front path, such that the front strap element is removable for replacement by an alternate front strap element. A funnel element may be adapted for connection to the frame at a lower rim of the grip at an installed position.

Abstract: A method of sorting cells including magnetically coupling a nozzle assembly to a flow cytometer in a predetermined orientation to form a fluid stream having a sheath fluid and a sample fluid. Particles within the sample fluid are oriented and then interrogated at an inspection zone to produces signals representative of emitted or reflected electromagnetic radiation. The electromagnetic radiation is analyzed to classify the cells and the cells are sorted according to their classification.