Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Abstract: In an illustrative embodiment, cell libraries created by rationally designed nucleic acids comprising sequences to facilitate nuclease-directed genome editing events are provided. The cell libraries include saturation mutagenesis cell libraries, promoter swap cell libraries, SNP swap cell libraries, terminator swap cell libraries, and combinations thereof.

Abstract: A method and device for the sorting and focusing of suspended particles is disclosed. The device has a micro-fluidic channel, at least one inlet and a number of outlets for providing, sorting and receiving particles. A patterned array of grooves is present inside the micro-fluidic channel. The inlets and outlets are connected to the micro-fluidic channel. The particles are sorted by the array of grooves. The method consists of providing particles in a flow-focused manner to one end of the micro-fluidic channel using at least one inlet. The particles are sorted by the array of grooves present in the micro-fluidic channel. Particles are collected by a number of outlets which are connected to the other end of the micro-fluidic channel.