Abstract: The disclosure features compositions and methods for the treatment of sensorineural hearing loss and auditory neuropathy, particularly forms of the disease that are associated with a mutation in otoferlin (OTOF), by way of OTOF gene therapy. The disclosure provides a variety of compositions that include a first nucleic acid vector that contains a polynucleotide encoding an N-terminal portion of an OTOF isoform 5 protein and a second nucleic acid vector that contains a polynucleotide encoding a C-terminal portion of an OTOF isoform 5 protein. These vectors can be used to increase the expression of OTOF in a subject, such as a human subject suffering from sensorineural hearing loss.

Abstract: A dishwasher comprising a chassis, a tub supported by the chassis and at least partially defining a treating chamber, and a pump that circulates wash liquid in the treating chamber. The pump has an impeller that has a cover, a base with an axis of rotation, and at least one fin protruding from the base. The impeller also has a cap removably coupled to the impeller and at least one blade integrally formed with the cap.

Abstract: The disclosure features compositions and methods for the treatment of sensorineural hearing loss and auditory neuropathy, particularly forms of the disease that are associated with mutations in otoferlin (OTOF), by way of OTOF gene therapy. The disclosure provides a variety of compositions that include a first nucleic acid vector that contains a polynucleotide encoding an N-terminal portion of an OTOF protein and a second nucleic acid vector that contains a polynucleotide encoding a C-terminal portion of an OTOF protein. These vectors can be used to increase the expression of OTOF in a subject, such as a human subject suffering from sensorineural hearing loss.

Abstract: The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

Abstract: The invention provides AAV1 vectors that can be used to transduce multiple inner ear cell types and their use for treatment of hearing loss, deafness, tinnitus, and vestibular dysfunction.

Abstract: A method and apparatus (3) for operating a rotary milking platform (1) to maximise the number of animals milked per unit time. The milking platform (1) is rotated about a central vertical axis (4) by a variable speed motor (6), and comprises a plurality of animal accommodating locations (5) for the animals being milked. An entry position (7) and an exit position (9) accommodate animals to and from the platform (1). A position sensor (10) monitors the angular position of the platform (1). An RFID sensor (12) reads the identity of animals entering the platform (1). Historical data relating to milking time per milking session and the milk yield per animal per session is stored in an electronic memory (17). A microprocessor (15) reads signals from flow meters (14) which monitor the milk flow from milking clusters of each animal accommodating location (5).

Abstract: The disclosure provides polynucleotides containing regions of the Myosin 15 (Myo15) promoter, as well as vectors containing the same, that can be used to promote expression of a transgene specifically in hair cells. The polynucleotides described herein may be operably linked to a transgene, such as a transgene encoding a therapeutic protein, so as to promote hair cell-specific expression of the transgene. The polynucleotides described herein may be operably linked to a therapeutic transgene and used for the treatment of subjects having or at risk of developing hearing loss or vestibular dysfunction.

Abstract: A dishwasher comprising a chassis, a tub supported by the chassis and at least partially defining a treating chamber, and a pump that circulates wash liquid in the treating chamber. The pump has an impeller that has a cover, a base with an axis of rotation, and at least one fin protruding from the base. The impeller also has a cap removably coupled to the impeller and at least one blade integrally formed with the cap.

Abstract: A dishwasher comprising a chassis, a tub supported by the chassis and at least partially defining a treating chamber, and a pump that circulates wash liquid in the treating chamber. The pump has an impeller that has a cover, a base with an axis of rotation, and at least one fin protruding from the base. The impeller also has a cap removably coupled to the impeller and at least one blade integrally formed with the cap.

Abstract: The disclosure features compositions and methods for the treatment of sensorineural hearing loss and auditory neuropathy, particularly forms of the disease that are associated with mutations in otoferlin (OTOF), by way of OTOF gene therapy. The disclosure provides a variety of compositions that include a first nucleic acid vector that contains a polynucleotide encoding an N-terminal portion of an OTOF protein and a second nucleic acid vector that contains a polynucleotide encoding a C-terminal portion of an OTOF protein. These vectors can be used to increase the expression of OTOF in a subject, such as a human subject suffering from sensorineural hearing loss.

Abstract: A method for continuously evaluating the effectiveness of debridement of a root canal of a tooth, the tooth having an open access cavity and an apex end, includes delivering a fluid to the open access cavity of the tooth, evacuating the fluid near the apex end of the tooth such that the fluid flushes most of the root canal before being evacuated, and continuously evaluating the evacuated fluid for at least one of a presence of debris, a concentration level of the debris, or a type of the debris. An apparatus for use in debriding a root canal of a tooth includes a microcannula or a macrocannula configured to evacuate a fluid in the root canal, and a sensing mechanism fluidically coupled to the microcannula or the macrocannula, the sensing mechanism configured to continuously sense debris in the evacuated fluid in real time.

Abstract: A dishwasher comprising a chassis, a tub supported by the chassis and at least partially defining a treating chamber, and a pump that circulates wash liquid in the treating chamber. The pump has an impeller that has a cover, a base with an axis of rotation, and at least one fin protruding from the base. The impeller also has a cap removably coupled to the impeller and at least one blade integrally formed with the cap.

Abstract: A method for continuously evaluating the effectiveness of debridement of a root canal of a tooth, the tooth having an open access cavity and an apex end, includes delivering a fluid to the open access cavity of the tooth, evacuating the fluid near the apex end of the tooth such that the fluid flushes most of the root canal before being evacuated, and continuously evaluating the evacuated fluid for at least one of a presence of debris, a concentration level of the debris, or a type of the debris. An apparatus for use in debriding a root canal of a tooth includes a microcannula or a macrocannula configured to evacuate a fluid in the root canal, and a sensing mechanism fluidically coupled to the microcannula or the macrocannula, the sensing mechanism configured to continuously sense debris in the evacuated fluid in real time.

Abstract: Plasma-enriched hydroxyapatite-based fillers and methods of filling bone gaps in oral surgery, orthopedic procedures, and cosmetic surgery are disclosed. The fillers contain a substance comprising an anticoagulant antidote, a substance comprising hydroxyapatite, and blood or plasma. The blood may be either autologous or allogeneic. The fillers have a dough-like consistency and can be manipulated easily by a surgeon.

Abstract: An indicator kit for detecting the presence of a quaternary ammonium compound disinfectant is provided. The kit includes an indicator composition that includes an azo dye material and water; an extraction composition that includes water, and at least one of a C1-C7 alcohol or a glycol ether; and an acidic compound present in at least one of the indicator composition, the extraction composition, or an acidic developer solution packaged separately from the indicator composition and the extraction composition. A method of detecting a quaternary ammonium compound disinfectant on a surface is also provided.

Abstract: A multifunctional tool having an interchangeable head is provided. The tool includes a pair of arms each having a jaw displacement end. A female pivot member having a central aperture is interdisposed between the jaw displacement ends and pivotally connects to each arm. At least two nodes are disposed on the front face and rear face of the jaw displacement end. An interchangeable head having a plurality of apertures and a male pivot member mates with the nodes of the female pivot member disposed on the jaw displacement end. A locking pin extends through the central apertures of the pivot members to attach the interchangeable head to the jaw displacement ends. When the interchangeable head is attached to the nodes on the front face, the tool functions as a clamp. When the interchangeable tool is attached to the nodes on the rear face, the tool functions as a separating apparatus.

Abstract: An indicator kit for detecting the presence of a quaternary ammonium compound disinfectant is provided. The kit includes an indicator composition that includes an azo dye material and water; an extraction composition that includes water, and at least one of a C1-C7 alcohol or a glycol ether; and an acidic compound present in at least one of the indicator composition, the extraction composition, or an acidic developer solution packaged separately from the indicator composition and the extraction composition. A method of detecting a quaternary ammonium compound disinfectant on a surface is also provided.

Abstract: An apparatus for visually isolating an observer from an observed individual comprises a two way mirror forming a first panel and a movable frame disposed to hold the first panel in a vertical orientation; methods of assembling and operating instructional simulation lab, methods for instruction/proctoring/operating/repurposing a nursing simulation lab comprising the steps of identifying a space having a simulated patient location and an observation location, providing a panel assembly having a two way mirror, locating the panel assembly between the simulated patient location and the observation location, providing a patent simulation system including a simulated patient, providing a lab student, providing a lab instructor and/or observer, administering care to the simulated patient at the simulated patient location, and observing the administration of care at the observation location by looking towards the simulated patient location through the two way mirror.