Abstract: The present disclosure relates to compositions and methods for separating and/or purifying polyribonucleotides. The polyribonucleotide may be separated from a mixture of polyribonucleotides with an oligonucleotide that hybridizes to a target region of the polyribonucleotide.

Abstract: The present disclosure is directed to methods for the enrichment of circular polyribonucleotides (circRNA), e.g., from population of polyribonucleotides containing circRNA and linear polyribonucleotides (linRNA), where the enrichment is performed under denaturing conditions. Also disclosed are compositions including a population of polyribonucleotides containing circRNA and linRNA in a solution under denaturing conditions. Further within the scope of the present disclosure are compositions containing an enriched population of circRNA, such as a composition that was produced by exposing the composition to one or more denaturing conditions.

Abstract: Syringe positioning apparatus and methods are disclosed that provide for automated positioning of a syringe at an axially aligned position on a predetermined axis. The apparatus and methods employ first and second members having opposing surfaces that include first and second ramps, respectively, wherein the first ramp angles upward and away from the second member, and the second ramp angles upward and away from the first member. At least one of the first and second members may be advanceable toward the other by an actuator, wherein the first and second ramps may slidably engage a syringe and thereby elevate the syringe to the axially aligned position on the predetermined axis.

Abstract: An apparatus and method for gripping a syringe utilizes four gripping members advanceable in different corresponding directions toward a predetermined axis to supportably engage a barrel of a syringe at corresponding circumferentially offset locations. A first motor may be utilized for co-driven advancement of the gripping members in tandem in the different corresponding directions toward the predetermined axis, and for retraction of the gripping members. The gripping members may include corresponding rollers, wherein a single one of the rollers may be rotated in an automated manner during supportive engagement of a syringe by the gripping members. A second motor may be utilized for driven rotation of the single roller. The disclosed embodiments may be utilized for supportive engagement of syringes having a range of syringe diameters.

Abstract: A merchant computer system receives a first token transmitted to the merchant computer system from a customer device. A request for a second token is transmitted from the merchant computer system to a payment processor computer. The request includes the first token. The merchant computer system receives the second token that it requested. The second token is different from the first token. The merchant computer system transmits a payment account system transaction request to the payment processor computer. The latter request includes the second token.

Abstract: A mechanism for patching functions in use on a running computer system. A method includes modifying an original function to call a hot patch function. The hot patch function transfers execution control from the original function to a replacement function.

Abstract: Methods and systems for characterization of NETosis (Neutrophil Extracellular Traps) in neutrophils. In some examples, a method includes acquiring an image of a sample of neutrophils. The method includes classifying each neutrophil depicted in the image as having either a NETotic or non-NETotic nucleus using one or more processors executing a convolutional neural network (CNN) trained on training images of NETotic and non-NETotic neutrophil nuclei. The method includes generating output indicative of a number of neutrophils classified as having a NETotic nucleus and a number of neutrophil classified as having a non-NETotic nucleus.

Abstract: An apparatus and method for gripping a syringe utilizes four gripping members advanceable in different corresponding directions toward a predetermined axis to supportably engage a barrel of a syringe at corresponding circumferentially offset locations. A first motor may be utilized for co-driven advancement of the gripping members in tandem in the different corresponding directions toward the predetermined axis, and for retraction of the gripping members. The gripping members may include corresponding rollers, wherein a single one of the rollers may be rotated in an automated manner during supportive engagement of a syringe by the gripping members. A second motor may be utilized for driven rotation of the single roller. The disclosed embodiments may be utilized for supportive engagement of syringes having a range of syringe diameters.

Abstract: Syringe positioning apparatus and methods are disclosed that provide for automated positioning of a syringe at an axially aligned position on a predetermined axis. The apparatus and methods employ first and second members having opposing surfaces that include first and second ramps, respectively, wherein the first ramp angles upward and away from the second member, and the second ramp angles upward and away from the first member. At least one of the first and second members may be advanceable toward the other by an actuator, wherein the first and second ramps may slidably engage a syringe and thereby elevate the syringe to the axially aligned position on the predetermined axis.

Abstract: Provided are a system and methods for reducing the amount of payment information stored by a merchant. In one example, a method includes receiving payment card information of a cardholder from a payment information receiving module displayed in a web browser of a computing device, generating a token for the payment card information received from the payment information receiving module, and transmitting the generated token to a merchant server for a transaction between the merchant and the cardholder. According to various aspects, the payment information receiving module is displayed in the web browser of the computing device in response to a merchant website being displayed therein, and the payment card information is received by the payment processing server, from the payment information receiving module, without being stored by the computing device and without passing through the merchant server.

Abstract: A medical liquid filling system and associated methods are provided, the system comprising a disposable and a drive system. The disposable includes a tubular member and a piston slidably disposed within the tubular member for retraction and advancement within at least a first portion of the tubular member. The disposable may include a seal member for sealing the first portion of the tubular member and may be interconnected to the piston for co-movement therewith. The drive system may include a piston drive member selectively interconnectable to the piston for driven advancement and retraction of the piston within the tubular member. The drive system may be interconnected to a controller, which may be operable to automate or semi-automate various filling operations. A user interface may be interconnected to the controller for providing input of various system operation parameters, which may be communicated to the controller to facilitate automated or semi-automated operation of the filling system.

Abstract: A medical liquid filling system and associated methods are provided, the system comprising a disposable and a drive system. The disposable includes a tubular member and a piston slidably disposed within the tubular member for retraction and advancement within at least a first portion of the tubular member. The disposable may include a seal member for sealing the first portion of the tubular member and may be interconnected to the piston for co-movement therewith. The drive system may include a piston drive member selectively interconnectable to the piston for driven advancement and retraction of the piston within the tubular member. The drive system may be interconnected to a controller, which may be operable to automate or semi-automate various filling operations. A user interface may be interconnected to the controller for providing input of various system operation parameters, which may be communicated to the controller to facilitate automated or semi-automated operation of the filling system.

Abstract: A medical liquid filling system and associated methods are provided, the system comprising a disposable and a drive system. The disposable includes a tubular member and a piston slidably disposed within the tubular member for retraction and advancement within at least a first portion of the tubular member. The disposable may include a seal member for sealing the first portion of the tubular member and may be interconnected to the piston for co-movement therewith. The drive system may include a piston drive member selectively interconnectable to the piston for driven advancement and retraction of the piston within the tubular member. The drive system may be interconnected to a controller, which may be operable to automate or semi-automate various filling operations. A user interface may be interconnected to the controller for providing input of various system operation parameters, which may be communicated to the controller to facilitate automated or semi-automated operation of the filling system.

Abstract: A medical liquid filling system and associated methods are provided, the system comprising a disposable and a drive system. The disposable includes a tubular member and a piston slidably disposed within the tubular member for retraction and advancement within at least a first portion of the tubular member. The disposable may include a seal member for sealing the first portion of the tubular member and may be interconnected to the piston for co-movement therewith. The drive system may include a piston drive member selectively interconnectable to the piston for driven advancement and retraction of the piston within the tubular member. The drive system may be interconnected to a controller, which may be operable to automate or semi-automate various filling operations. A user interface may be interconnected to the controller for providing input of various system operation parameters, which may be communicated to the controller to facilitate automated or semi-automated operation of the filing system.

Abstract: A medical liquid filling system and associated methods are provided, the system comprising a disposable and a drive system. The disposable includes a tubular member and a piston slidably disposed within the tubular member for retraction and advancement within at least a first portion of the tubular member. The disposable may include a seal member for sealing the first portion of the tubular member and may be interconnected to the piston for co-movement therewith. The drive system may include a piston drive member selectively interconnectable to the piston for driven advancement and retraction of the piston within the tubular member. The drive system may be interconnected to a controller, which may be operable to automate or semi-automate various filling operations. A user interface may be interconnected to the controller for providing input of various system operation parameters, which may be communicated to the controller to facilitate automated or semi-automated operation of the filling system.

Abstract: In one or more embodiments, an optical fiber coupler for coupling pump radiation into a rectangular optical fiber includes a fiber section and a pump fiber. The fiber section includes a core having a high aspect ratio cross-section and an interface section. The core is positioned in contact with first and second signal claddings to form reflective boundaries at fast-axis boundaries of the core. The pump fiber is side coupled to the fiber section via the interface section, and configured to couple the pump radiation into the fiber section.

Abstract: In one or more embodiments, an optical fiber coupler for coupling pump radiation into a rectangular optical fiber includes a fiber section and a pump fiber. The fiber section includes a core having a high aspect ratio cross-section and an interface section. The core is positioned in contact with first and second signal claddings to form reflective boundaries at fast-axis boundaries of the core. The pump fiber is side coupled to the fiber section via the interface section, and configured to couple the pump radiation into the fiber section.

Abstract: A method and system for providing a computer system having a plurality of logical partitions with a virtual terminal is disclosed. The computer system is coupled to an external display device through a single communications interface. The method and system include allowing the logical partitions to share the communications interface by multiplexing communications to the display device for each of the logical partitions over the communication interface. Windows are displayed on the display device for each of the logical partitions, and the communications from each of the logical partitions is then routed to the corresponding window for display on the display device.

Abstract: A method and apparatus for providing a computer system having a plurality of logical partitions with a virtual operator panel is disclosed. The method and apparatus include displaying a plurality of operator panels on a single console corresponding to each of the logical partitions, and providing a buffer for each logical partition. The status codes from each of the logical partitions are then written directly to the corresponding buffer. To display the status codes of one of the logical partitions, the status code from the buffer corresponding the logical partition is read and sent to the corresponding operator panel for display.

Abstract: A tubing guide for directing coiled tubing through an injector apparatus and into a well. The tubing guide comprises a frame, which is also referred to as a tubing carrier, extending from a base. The shape of the tubing guide changes depending upon the natural radius of curvature of the tubing. The tubing carrier will conform to provide a path for the coiled tubing that more nearly approximates the residual radius of curvature of the coiled tubing. The carrier has a plurality of segments pivotably connected to one another. The segments can pivot and thus the carrier itself can move from a fully retracted position to a fully rotated position. In the fully rotated position, the carrier can approximate a radius larger than that in the fully closed position. The segments are allowed to pivot or rotate as the tubing is passing therethrough so that the shape of the carrier is continually adjusting so as to provide a more natural path for the coiled tubing.