Patents by Inventor Joshua Trueheart

Joshua Trueheart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Optimization of C-8 sterol isomerization
    Patent number: 12049617
    Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.
    Type: Grant
    Filed: May 21, 2019
    Date of Patent: July 30, 2024
    Assignee: DSM IP Assets B.V.
    Inventors: Christopher Mark Farrell, Lisa Ann Laprade, Otto Martin Lehmann, Joshua Trueheart, Bastien Jean Wolfgang Chevreux
  • Production of retinyl esters
    Patent number: 12049658
    Abstract: The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: July 30, 2024
    Assignee: DSM IP Assets B.V.
    Inventors: Nathalie Balch, Paul Blomquist, Reed Doten, Peter Houston, Ethan Lam, Jenna Mcmahon, Joshua Trueheart, Celine Viarouge, René Marcel De Jong
  • BIOSYNTHESIS OF ISOPRENOIDS AND PRECURSORS THEREOF
    Publication number: 20240218403
    Abstract: Described in this application are proteins and host cells involved in methods of producing isoprenoid precursors and/or isoprenoids.
    Type: Application
    Filed: April 1, 2022
    Publication date: July 4, 2024
    Applicant: Ginkgo Bioworks, Inc.
    Inventors: Guillaume Beaudoin, Elena Brevnova, Alkiviadis Orfefs Chatzivasileiou, Alexandra Exner, Annapuma Kamineni, Matthew McMahon, Joshua Trueheart
  • PRODUCTION OF RETINOL
    Publication number: 20240218336
    Abstract: The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A.
    Type: Application
    Filed: February 13, 2024
    Publication date: July 4, 2024
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE
  • Optimization of C-5 sterol desaturation
    Patent number: 12018309
    Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.
    Type: Grant
    Filed: May 21, 2019
    Date of Patent: June 25, 2024
    Assignee: DSM IP Assets B.V.
    Inventors: Christopher Mark Farrell, Lisa Ann Laprade, Otto Martin Lehmann, Joshua Trueheart, Bastien Jean Wolfgang Chevreux
  • BIOSYNTHESIS OF MOGROSIDES
    Publication number: 20240200114
    Abstract: Described in this application are proteins and host cells involved in methods of producing mogrol precursors, mogrol, and/or mogrosides.
    Type: Application
    Filed: April 1, 2022
    Publication date: June 20, 2024
    Applicant: GINKGO BIOWORKS, INC.
    Inventors: Guillaume Beaudoin, Alexandra Exner, Annapurna Kamineni, Matthew McMahon, Joshua Trueheart
  • Production of retinyl esters
    Patent number: 11905542
    Abstract: The present invention is related to a novel enzymatic process for production of retinyl esters, such as in particular retinyl long chain esters, via conversion of retinol, which process includes the use of enzymes having acyltransferase activity. Said process might be used for biotechnological production of vitamin A.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: February 20, 2024
    Assignee: DSM IP ASSETS B.V.
    Inventors: Nathalie Balch, Paul Blomquist, Reed Doten, Peter Houston, Ethan Lam, Jenna McMahon, Joshua Trueheart, Celine Viarouge
  • BIOSYNTHESIS OF RETINOIDS
    Publication number: 20230227862
    Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.
    Type: Application
    Filed: January 30, 2023
    Publication date: July 20, 2023
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE
  • Biosynthesis of retinoids
    Patent number: 11578344
    Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.
    Type: Grant
    Filed: September 25, 2018
    Date of Patent: February 14, 2023
    Assignee: DSM IP ASSETS B.V.
    Inventors: Nathalie Balch, Paul Blomquist, Reed Doten, Peter Houston, Ethan Lam, Jenna McMahon, Joshua Trueheart, Celine Viarouge
  • OPTIMIZATION OF C-8 STEROL ISOMERIZATION
    Publication number: 20210238539
    Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.
    Type: Application
    Filed: May 21, 2019
    Publication date: August 5, 2021
    Inventors: Christopher Mark FARRELL, Lisa Ann LAPRADE, Otto Martin LEHMANN, Joshua TRUEHEART, Bastien Jean Wolfgang CHEVREUX
  • OPTIMIZATION OF C-5 STEROL DESATURATION
    Publication number: 20210222222
    Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.
    Type: Application
    Filed: May 21, 2019
    Publication date: July 22, 2021
    Inventors: Christopher Mark FARRELL, Lisa Ann LAPRADE, Otto Martin LEHMANN, Joshua TRUEHEART, Bastien Jean Wolfgang CHEVREUX
  • MODIFIED STEROL ACYLTRANSFERASES
    Publication number: 20210115412
    Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a yeast strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.
    Type: Application
    Filed: May 21, 2019
    Publication date: April 22, 2021
    Inventors: Christopher Mark FARRELL, Lisa Ann LAPRADE, Otto Martin LEHMANN, Joshua TRUEHEART
  • PRODUCTION OF TRANS-RETINAL
    Publication number: 20200277644
    Abstract: The present invention is related to a novel enzymatic process for production of vitamin A aldehyde (retinal) via stereoselective conversion of beta-carotene which process includes the use of trans-selective enzymes having activity as beta-carotene oxidases (BCOs), in particular having preference for trans-retinal. 5 Said process is in particular useful for biotechnological production of vitamin A.
    Type: Application
    Filed: September 25, 2018
    Publication date: September 3, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE, René Marcel DE JONG
  • PRODUCTION OF RETINOL
    Publication number: 20200248151
    Abstract: The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A.
    Type: Application
    Filed: September 25, 2018
    Publication date: August 6, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE
  • PRODUCTION OF RETINYL ESTERS
    Publication number: 20200239925
    Abstract: The present invention is related to production of retinyl esters, such as in particular retinyl acetate, an important building block for production of vitamin A. The retinyl esters might be generated via enzymatic conversion of retinol which process includes the use of enzymes with acetyl-transferase (ATF) activity, thus acetylating retinol into retinol/retinyl acetate. Said process is particularly useful for biotechnological production of vitamin A.
    Type: Application
    Filed: September 25, 2018
    Publication date: July 30, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE, René Marcel DE JONG
  • PRODUCTION OF RETINYL ESTERS
    Publication number: 20200239924
    Abstract: The present invention is related to a novel enzymatic process for production of retinyl esters, such as in particular retinyl long chain esters, via conversion of retinol, which process includes the use of enzymes having acyltransferase activity. Said process might be used for biotechnological production of vitamin A.
    Type: Application
    Filed: September 25, 2018
    Publication date: July 30, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE
  • BIOSYNTHESIS OF RETINOIDS
    Publication number: 20200231993
    Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.
    Type: Application
    Filed: September 25, 2018
    Publication date: July 23, 2020
    Inventors: Nathalie BALCH, Paul BLOMQUIST, Reed DOTEN, Peter HOUSTON, Ethan LAM, Jenna MCMAHON, Joshua TRUEHEART, Celine VIAROUGE
  • MATING TYPE SWITCH IN YARROWIA LIPOLYTICA
    Publication number: 20200024609
    Abstract: The present invention is directed to a process for switching the mating type of a Yarrowia fungus strain into an opposite mating type, and to the use thereof to sexually cross two individual strains resulting in new strains.
    Type: Application
    Filed: May 17, 2019
    Publication date: January 23, 2020
    Inventors: Adam G. LAWRENCE, Peter Louis Houston, Jessica Leigh MCGRATH, Joshua Trueheart
  • Use of thermophilic nucleases for degrading nucleic acids
    Patent number: 10385308
    Abstract: The present invention relates to the use of a thermophilic nuclease for degrading nucleic acids in vivo and/or in situ, wherein the thermophilic nuclease is heterologous to the host cell and is produced by the host rather than being added exogenously. The present invention further relates to a genetically modified cell which was produced according to the above method. The present invention is particularly beneficial in inactivating the biological activity of recombinant DNA in biomass or biomass-derived products.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: August 20, 2019
    Assignee: DSM IP Assets B.V.
    Inventors: Joshua Trueheart, Jessica McGrath
  • MATING TYPE SWITCH IN YARROWIA LIPOLYTICA
    Publication number: 20190010508
    Abstract: The present invention is directed to a process for switching the mating type of a Yarrowia fungus strain into an opposite mating type, and to the use thereof to sexually cross two individual strains resulting in new strains.
    Type: Application
    Filed: January 6, 2017
    Publication date: January 10, 2019
    Inventors: Adam G. LAWRENCE, Peter Louis HOUSTON, Jessica Leigh MCGRATH, Joshua TRUEHEART