Patents by Inventor Jude Samulski

Jude Samulski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cell Line for Production of Adeno-associated Virus
    Publication number: 20140242671
    Abstract: This invention relates to a HEK293 cell line that grows under animal component-free suspension conditions. The cell line is ideal for rapid and scalable production of adeno-associated virus (AAV) and supports production of all serotypes and chimera of AAV.
    Type: Application
    Filed: October 26, 2012
    Publication date: August 28, 2014
    Inventors: Joshua Grieger, Richard Jude Samulski
  • Helper virus-free AAV production
    Patent number: 8809058
    Abstract: A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
    Type: Grant
    Filed: June 17, 2008
    Date of Patent: August 19, 2014
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Forrest K. Ferrari, Xiao Xiao, Richard Jude Samulski
  • Duplexed parvovirus vectors
    Patent number: 8784799
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: July 22, 2014
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Duplexed Parvovirus Vectors
    Publication number: 20130252325
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Application
    Filed: January 28, 2013
    Publication date: September 26, 2013
    Applicant: The University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Restrictive Inverted Terminal Repeats for Viral Vectors
    Publication number: 20130109742
    Abstract: This invention relates to modified parvovirus inverted terminal repeats (ITRs) that do not functionally interact with wild-type large Rep proteins, synthetic Rep proteins that functionally interact with the modified ITRs, and methods of using the same for delivery of nucleic acids to a cell or a subject. The modifications provide a novel Rep-ITR interaction that limits vector mobilization, increasing the safety of viral vectors.
    Type: Application
    Filed: January 12, 2011
    Publication date: May 2, 2013
    Inventors: Curtis Hewitt, Richard Jude Samulski
  • Methods for fabricating isolated micro- and nano-structures using soft or imprint lithography
    Patent number: 8420124
    Abstract: The presently disclosed subject matter describes the use of fluorinated elastomer-based materials, in particular perfluoropolyether (PFPE)-based materials, in high-resolution soft or imprint lithographic applications, such as micro- and nanoscale replica molding, and the first nano-contact molding of organic materials to generate high fidelity features using an elastomeric mold. Accordingly, the presently disclosed subject matter describes a method for producing free-standing, isolated nanostructures of any shape using soft or imprint lithography technique.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: April 16, 2013
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Joseph M. DeSimone, Jason P. Rolland, Benjamin W. Maynor, Larkin E. Euliss, Ginger Denison Rothrock, Ansley E. Dennis, Edward T. Samulski, R. Jude Samulski
  • Duplexed parvovirus vectors
    Patent number: 8361457
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Grant
    Filed: August 2, 2010
    Date of Patent: January 29, 2013
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Compositions and Methods for Enhanced Parvovirus Transduction
    Publication number: 20130012574
    Abstract: The present invention provides methods and compositions for enhanced transduction of an adeno-associated virus (AAV) vector comprising a heterologous nucleic acid of interest wherein the AAV vector genome is oversized relative to a wild type AAV genome by employing a proteasome inhibitor.
    Type: Application
    Filed: February 4, 2011
    Publication date: January 10, 2013
    Inventors: Paul E. Monahan, Richard Jude Samulski
  • Methods for fabricating isolated micro-and nano-structures using soft or imprint lithography
    Patent number: 8263129
    Abstract: The presently disclosed subject matter describes the use of fluorinated elastomer-based materials, in particular perfluoropolyether (PFPE)-based materials, in high-resolution soft or imprint lithographic applications, such as micro- and nanoscale replica molding, and the first nano-contact molding of organic materials to generate high fidelity features using an elastomeric mold. Accordingly, the presently disclosed subject matter describes a method for producing free-standing, isolated nanostructures of any shape using soft or imprint lithography techniques.
    Type: Grant
    Filed: December 20, 2004
    Date of Patent: September 11, 2012
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Joseph M. DeSimone, Jason P. Rolland, Benjamin W. Maynor, Larken E. Euliss, Ginger Denison Rothrock, Ansley E Dennis, Edward T. Samulski, R. Jude Samulski
  • Modified Virus Vectors and Methods of Making and Using the Same
    Publication number: 20120009268
    Abstract: The present invention provides AAV capsid proteins (VP1, VP2 and/or VP3) comprising a modification in the amino acid sequence in the three-fold axis loop 4 and virus capsids and virus vectors comprising the modified AAV capsid protein. In particular embodiments, the modification comprises a substitution of one or more amino acids at amino acid positions 585 to 590 (inclusive) of the native AAV2 capsid protein sequence or the corresponding positions of other AAV capsid proteins. The invention also provides methods of administering the virus vectors and virus capsids of the invention to a cell or to a subject in vivo.
    Type: Application
    Filed: February 11, 2010
    Publication date: January 12, 2012
    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
    Inventors: Aravind Asokan, Richard Jude Samulski
  • Nanoparticle fabrication methods, systems, and materials
    Publication number: 20110182805
    Abstract: Nano-particles are molded in nano-scale molds fabricated from non-wetting, low surface energy polymeric materials. The nano-particles can include pharmaceutical compositions, taggants, contrast agents, biologic drugs, drug compositions, organic materials, and the like. The molds can be virtually any shape and less than 10 micron in cross-sectional diameter.
    Type: Application
    Filed: June 19, 2006
    Publication date: July 28, 2011
    Inventors: Joseph M DeSimone, Jason P. Rolland, Ansley Exner Dennis, Edward T. Samulski, R. Jude Samulski, Benjamin W. Maynor, Larken E. Euliss, Ginger Denison Rothrock, Stephanie Barrett, Alexander Ermoshkin, Andrew James Murphy
  • Chimeric Vectors
    Publication number: 20110104119
    Abstract: The present invention is based, in part, on the discovery that parvovirus (including AAV) capsids can be engineered to incorporate small, selective regions from other parvoviruses that confer desirable properties. The inventors have discovered that in some cases as little as a single amino acid insertion or substitution from a first parvovirus (e.g., an AAV) into the capsid structure of another parvovirus (e.g., an AAV) to create a chimeric parvovirus is sufficient to confer one or more of the desirable properties of the first parvovirus to the resulting chimeric parvovirus and/or to confer a property that is not exhibited by the first parvovirus or is present to a lesser extent.
    Type: Application
    Filed: January 3, 2011
    Publication date: May 5, 2011
    Inventors: Dawn E. Bowles, Chengwen Li, Joseph E. Rabinowitz, Josh Grieger, Mavis Agbandje-McKenna, Richard Jude Samulski
  • Methods and compounds for controlled release of recombinant parvovirus vectors
    Patent number: 7923436
    Abstract: The invention uses recombinant parvoviruses, and particularly recombinant adeno-associated virus (rAAV) to deliver genes and DNA sequences for gene therapy following manipulation of the therapeutic virus for packaging and transport. The invention delivers therapeutic viral vectors via rAAV affixed to support matrixes (i.e., sutures, surgically implantable materials, grafts, and the like).
    Type: Grant
    Filed: April 5, 2007
    Date of Patent: April 12, 2011
    Assignee: University of North Carolina - Chapel Hill
    Inventors: Paul E. Monahan, Richard Jude Samulski, Joseph E. Rabinowitz
  • Chimeric vectors
    Patent number: 7892809
    Abstract: The present invention is based, in part, on the discovery that parvovirus (including AAV) capsids can be engineered to incorporate small, selective regions from other parvoviruses that confer desirable properties. The inventors have discovered that in some cases as little as a single amino acid insertion or substitution from a first parvovirus (e.g., an AAV) into the capsid structure of another parvovirus (e.g., an AAV) to create a chimeric parvovirus is sufficient to confer one or more of the desirable properties of the first parvovirus to the resulting chimeric parvovirus and/or to confer a property that is not exhibited by the first parvovirus or is present to a lesser extent.
    Type: Grant
    Filed: December 15, 2005
    Date of Patent: February 22, 2011
    Assignees: The University of North Carolina at Chapel Hill, The University of Florida Research Foundation
    Inventors: Dawn E. Bowles, Chengwen Li, Joseph E. Rabinowitz, Josh Grieger, Mavis Agbandje-McKenna, Richard Jude Samulski
  • Heparin and heparan sulfate binding chimeric vectors
    Patent number: 7867484
    Abstract: The present invention is based on the finding that parvovirus (including AAV) capsids can be engineered to incorporate small, selective regions from other parvoviruses that confer desirable properties. In some embodiments, a substitution of a single amino acid that is unique to the AAV6 capsid (Lys-531) among other AAVs that have been characterized to date can confer one or more desirable properties to other AAVs.
    Type: Grant
    Filed: January 26, 2007
    Date of Patent: January 11, 2011
    Assignee: University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Zhijian Wu, Aravind Asokan
  • Duplexed Parvovirus Vectors
    Publication number: 20100310516
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Application
    Filed: August 2, 2010
    Publication date: December 9, 2010
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • Methods and compositions for regulated expression of multiple nucleic acids
    Publication number: 20100303776
    Abstract: The present invention provides a cell comprising a first heterologous nucleic acid construct and a second heterologous nucleic acid construct, wherein each of said first and second heterologous nucleic acid constructs comprises: A. a first nucleotide sequence encoding a nucleotide sequence of interest (NOI); and B.
    Type: Application
    Filed: April 16, 2010
    Publication date: December 2, 2010
    Inventors: Richard Jude Samulski, Kyson Xiaohuai Chou
  • Duplexed parvovirus vectors
    Patent number: 7790154
    Abstract: The present invention provides duplexed parvovirus vector genomes that are capable under appropriate conditions of forming a double-stranded molecule by intrastrand base-pairing. Also provided are duplexed parvovirus particles comprising the vector genome. Further disclosed are templates and methods for producing the duplexed vector genomes and duplexed parvovirus particles of the invention. Methods of administering these reagents to a cell or subject are also described. Preferably, the parvovirus capsid is an AAV capsid. It is further preferred that the vector genome comprises AAV terminal repeat sequences.
    Type: Grant
    Filed: January 19, 2007
    Date of Patent: September 7, 2010
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Richard Jude Samulski, Douglas M. McCarty
  • METHODS AND COMPOSITIONS FOR INTRA-ARTICULAR COAGULATION PROTEINS
    Publication number: 20100137211
    Abstract: The present invention provides methods and compositions for treating blood clotting factor disorders and/or reducing bleeding-associated joint damage by treatments delivered to the joint in a subject.
    Type: Application
    Filed: April 11, 2008
    Publication date: June 3, 2010
    Inventors: Paul E. Monahan, Richard Jude Samulski, Darrel W. Stafford
  • METHODS AND COMPOSITIONS FOR ADENO-ASSOCIATED VIRUS (AAV) WITH HI LOOP MUTATIONS
    Publication number: 20090215879
    Abstract: The invention provides modified AAV capsid proteins comprising substitutions in the HI loop. Suitable substitutions include affinity tags, sequences that facilitate detection and/or targeting peptides. The invention also provides virus capsids and virus vectors comprising the modified AAV capsid proteins and methods of using the same. Further provided are methods of purifying the modified AAV capsid subunits, virus capsids and virus vectors of the invention.
    Type: Application
    Filed: February 12, 2009
    Publication date: August 27, 2009
    Inventors: NINA DIPRIMIO, RICHARD JUDE SAMULSKI