Patents by Inventor Juergen Siekmann

Juergen Siekmann has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DETERMINATION OF MODIFICATION DEGREE OF THERAPEUTIC PROTEINS USING 1H-NMR SPECTROSCOPY
    Publication number: 20230096592
    Abstract: This invention provides, among other things, methods for determining the average number of first polymers covalently attached to protein-first polymer conjugates in a solution.
    Type: Application
    Filed: February 24, 2021
    Publication date: March 30, 2023
    Inventors: Jean-Baptiste Farcet, Juergen Siekmann
  • Nucleophilic catalysts for oxime linkage
    Patent number: 11564992
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Grant
    Filed: January 21, 2020
    Date of Patent: January 31, 2023
    Assignee: Takeda Pharmaceutical Company Limited
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • THERAPEUTIC PROTEINS WITH INCREASED HALF-LIFE AND METHODS OF PREPARING SAME
    Publication number: 20220175939
    Abstract: The present disclosure relates to materials and methods of conjugating a water soluble polymer to a therapeutic protein.
    Type: Application
    Filed: February 22, 2022
    Publication date: June 9, 2022
    Applicants: Baxalta Incorporated, Baxalta GmbH
    Inventors: Juergen Siekmann, Alfred Weber, Hanspeter Rottensteiner, Peter Turecek
  • Blood coagulation protein conjugates
    Patent number: 11040109
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a blood coagulation protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer. In one embodiment of the invention the conjugation is carried out in the presence of the nucleophilic catalyst aniline. In addition the generated oxime linkage can be stabilized by reduction with NaCNBH3 to form an alkoxyamine linkage.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: June 22, 2021
    Assignee: Takeda Pharmaceutical Company Limited
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • NUCLEOPHILIC CATALYSTS FOR OXIME LINKAGE
    Publication number: 20210163527
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Application
    Filed: February 5, 2021
    Publication date: June 3, 2021
    Inventors: Stefan Haider, Andreas Ivens, Hanspeter Rottensteiner, Jüergen Siekmann, Peter Turecek, Oliver Zoechling
  • Factor VIII polymer conjugates
    Patent number: 11020458
    Abstract: The invention is a proteinaceous construct comprising a Factor VIII molecule which is conjugated to a water-soluble polymer via carbohydrate moieties of Factor VIII, and methods of preparing same.
    Type: Grant
    Filed: February 26, 2019
    Date of Patent: June 1, 2021
    Assignee: Takeda Pharmaceutical Company Limited
    Inventors: Peter Turecek, Juergen Siekmann
  • THERAPEUTIC PROTEINS WITH INCREASED HALF-LIFE AND METHODS OF PREPARING SAME
    Publication number: 20210106691
    Abstract: The present disclosure relates to materials and methods of conjugating a water soluble polymer to a therapeutic protein.
    Type: Application
    Filed: October 5, 2020
    Publication date: April 15, 2021
    Inventors: Juergen Siekmann, Alfred Weber, Hanspeter Rottensteiner, Peter Turecek
  • Glycopolysialylation of non-blood coagulation proteins
    Patent number: 10772968
    Abstract: A water soluble polymer, in particular polysialic acid (PSA) or a modified PSA (mPSA), is conjugated to an oxidized carbohydrate moiety of a glycoprotein other than a blood coagulation protein or to a ganglioside or drug delivery system by contacting the oxidized carbohydrate moiety with the water soluble polymer, wherein said water soluble polymer contains an aminooxy group and an oxime linkage is formed between the oxidized carbohydrate moiety and the aminooxy group on the water soluble polymer or wherein said water soluble polymer contains a hydrazide group and a hydrazone linkage is formed between the oxidized carbohydrate moiety and the hydrazide group on the water soluble polymer. Conjugates of aminooxy- or hydrazide-water soluble polymer, such as PSA and mPSA, are thus obtained in which the PSA or mPSA is attached via a carbohydrate moiety.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: September 15, 2020
    Assignees: Lipoxen Technologies Limited, Baxalta Incorporated, Baxalta GmbH
    Inventors: Sanjay Jain, Gregory Gregoriadis, Archana Dwivedi, Srijit Nath, Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • NUCLEOPHILIC CATALYSTS FOR OXIME LINKAGE
    Publication number: 20200230252
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Application
    Filed: January 21, 2020
    Publication date: July 23, 2020
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • Nucleophilic catalysts for oxime linkage
    Patent number: 10576160
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Grant
    Filed: July 7, 2017
    Date of Patent: March 3, 2020
    Assignees: Baxalta Incorporated, Baxalta GmbH
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • NUCLEOPHILIC CATALYSTS FOR OXIME LINKAGE
    Publication number: 20200017543
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Application
    Filed: August 5, 2019
    Publication date: January 16, 2020
    Inventors: Stefan Haider, Andreas Ivens, Hanspeter Rottensteiner, Jürgen Siekmann, Peter Turecek, Oliver Zoechling
  • BLOOD COAGULATION PROTEIN CONJUGATES
    Publication number: 20190314516
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a blood coagulation protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer. In one embodiment of the invention the conjugation is carried out in the presence of the nucleophilic catalyst aniline. In addition the generated oxime linkage can be stabilized by reduction with NaCNBH3 to form an alkoxyamine linkage.
    Type: Application
    Filed: June 20, 2019
    Publication date: October 17, 2019
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • Nucleophilic catalysts for oxime linkage
    Patent number: 10414793
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: September 17, 2019
    Assignees: Baxalta Incorporated, Baxalta GmbH
    Inventors: Stefan Haider, Andreas Ivens, Hanspeter Rottensteiner, Juergen Siekmann, Peter Turecek, Oliver Zoechling
  • FACTOR VIII WITH EXTENDED HALF-LIFE AND REDUCED LIGAND-BINDING PROPERTIES
    Publication number: 20190240295
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to a modified, recombinant Factor VIII (FVIII) with extended half-life and reduced ligand-binding properties.
    Type: Application
    Filed: February 22, 2019
    Publication date: August 8, 2019
    Inventors: Peter Turecek, Gerald Schrenk, Juergen Siekmann
  • Blood coagulation protein conjugates
    Patent number: 10350301
    Abstract: The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a blood coagulation protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer. In one embodiment of the invention the conjugation is carried out in the presence of the nucleophilic catalyst aniline. In addition the generated oxime linkage can be stabilized by reduction with NaCNBH3 to form an alkoxyamine linkage.
    Type: Grant
    Filed: August 29, 2016
    Date of Patent: July 16, 2019
    Assignees: Baxalta Incorporated, Baxalta GmbH
    Inventors: Juergen Siekmann, Stefan Haider, Hanspeter Rottensteiner, Peter Turecek
  • FACTOR VIII POLYMER CONJUGATES
    Publication number: 20190183981
    Abstract: The invention is a proteinaceous construct comprising a Factor VIII molecule which is conjugated to a water-soluble polymer via carbohydrate moieties of Factor VIII, and methods of preparing same.
    Type: Application
    Filed: February 26, 2019
    Publication date: June 20, 2019
    Inventors: Peter Turecek, Juergen Siekmann
  • MODIFIED BLOOD FACTORS COMPRISING A LOW DEGREE OF WATER SOLUBLE POLYMER
    Publication number: 20190160178
    Abstract: The present invention relates, in general, to materials and methods for the preparation of modified blood factors which have low levels of water soluble polymer molecules conjugated to the blood factor but exhibit biological activity similar to or better than molecules having a higher number of water soluble polymer moieties.
    Type: Application
    Filed: October 30, 2018
    Publication date: May 30, 2019
    Inventors: Peter Turecek, Juergen Siekmann, Hanspeter Rottensteiner
  • THERAPEUTIC PROTEINS WITH INCREASED HALF-LIFE AND METHODS OF PREPARING SAME
    Publication number: 20190142958
    Abstract: The present disclosure relates to materials and methods of conjugating a water soluble polymer to a therapeutic protein.
    Type: Application
    Filed: July 11, 2018
    Publication date: May 16, 2019
    Inventors: Juergen Siekmann, Alfred Weber, Hanspeter Rottensteiner, Peter Turecek
  • Factor VIIa-polysialic acid conjugates having prolonged in vivo half-life
    Patent number: 10251941
    Abstract: The present invention relates to a proteinaceous construct comprising plasmatic or recombinant factor VIIa (FVIIa) or biologically active derivatives thereof, which are bound to a carbohydrate moiety comprising 1-4 sialic acid units, wherein the in vivo half-life of the proteinaceous construct is substantially prolonged in the blood of a mammal, as compared to the in vivo half-life of a FVIIa molecule not bound to a carbohydrate moiety. The invention also provides a method for controlling bleeding in a mammal having a bleeding disorder due to functional defects or deficiencies of FVIIa, FVIII, or FIX. The invention also provides a method for controlling bleeding in a mammal during surgery or trauma.
    Type: Grant
    Filed: November 19, 2015
    Date of Patent: April 9, 2019
    Assignees: Baxalta Incorporated, Baxalta GmbH
    Inventors: Peter Turecek, Juergen Siekmann, Friedrich Scheiflinger, Michel Canavaggio
  • THERAPEUTIC PROTEINS WITH INCREASED HALF-LIFE AND METHODS OF PREPARING SAME
    Publication number: 20180318432
    Abstract: The present disclosure relates to materials and methods of conjugating a water soluble polymer to a therapeutic protein.
    Type: Application
    Filed: July 11, 2018
    Publication date: November 8, 2018
    Inventors: Juergen Siekmann, Alfred Weber, Hanspeter Rottensteiner, Peter Turecek