Abstract: Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, flits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals.

Abstract: Antibodies and meditopes that bind to the antibodies are provided, as well as complexes, compositions and combinations containing the meditopes and antibodies, and methods of producing, using, testing, and screening the same, including therapeutic and diagnostic methods and uses.

Abstract: A method and device for recording data of a terminal are disclosed. The method includes: setting a relation condition list for triggering recording among function modules, and setting a corresponding random probability generator for each function module; determining whether to start recording data, and whether to end the data recording operation in accordance with the relation condition list or the random probability generator, during the operation process of each function module; and sending the respective data packets obtained by recording to a data management module for storage after the data record operation of each function module is ended. By adopting the method and device, fuzzy recording can be performed on data of the terminal, and data of different function modules can be stored centrally, and a query operation is simplified.

Abstract: The invention discloses a method for obtaining calibration parameters of a touch screen comprising displaying at least two preset calibration reference points on a LCD; obtaining physical coordinates of sampling points obtained by clicking on positions corresponding to the calibration reference points on the touch screen and displaying the sampling points according to the physical coordinates and the calibration parameters; and if it is determined that the displayed sampling points are not coincident with their corresponding calibration reference points, adjusting distances between the sampling points and their corresponding calibration reference points, determining a minimal deviation between the adjusted sampling points and their corresponding calibration reference points and applying the minimal deviation to update the calibration parameters. The invention also provides an apparatus for obtaining calibration parameters of a touch screen.

Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.

Abstract: A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided.

Abstract: The invention in some aspects relates to recombinant adeno-associated viruses having distinct tissue targeting capabilities. In some aspects, the invention relates to gene transfer methods using the recombinant adeno-associate viruses. In some aspects, the invention relates to isolated AAV capsid proteins and isolated nucleic acids encoding the same.

Abstract: Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated.

Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.

Abstract: A microfluidic device for batch processing magnetic bead assays and having one or more microfluidic sample channels, comprising, one or more micromagnets seated in a fixture; and an actuator; wherein a portion of each micromagnet is in releasable operative association with one or more of the microfluidic sample channels, and another portion of each micromagnet is in releasable operative association with the actuator; and methods for using the same.

Abstract: An enclosure for generating a secondary environment within a processing chamber for coating a substrate. An enclosure wall forms a secondary environment encompassing the coating source, plasma, and the substrate, and separating them from interior of the processing chamber. The enclosure wall includes a plurality of pumping channels for diverting gaseous flow away from the substrate. The channels have an intake of larger diameter from the exhaust opening and are oriented at an angle with the intake opening pointing away from the deposition source. A movable seal enables transport of the substrate in open position and processing the substrate in closed position. The seal may be formed as a labyrinth seal to avoid particle generation from a standard contact seal.

Abstract: A fluid processing device is provided that includes a substrate, a plurality of fluid retainment regions formed in or on the substrate, and a barrier at least partially separating two or more of the fluid retainment regions. The barrier includes a mixture of a sequestering material and a reaction component. The reaction component can be at least one of a reactant, a reagent, a catalyst, an initiator, a promoter, a cofactor, an enzyme, a salt, or a combination thereof. The sequestering material can be a porous material, a dissolvable material, or both.

Abstract: Disclosed are violet, blue, and green phosphors having excellent durability and high luminance. Specifically disclosed is a phosphor which contains a metal element M (M is at least one element selected from among Mn, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Tm and Yb) for constituting a metal ion, which is solid-solubilized in an AlON crystal, an AlON solid solution crystal or an inorganic crystal having the same crystal structure as AlON. The phosphor is capable of emitting fluorescence having a peak in the wavelength range from 300 nm to 700 nm. Also disclosed is a method for producing such a phosphor. Further disclosed are an illuminating device and an image display each containing such a phosphor.

Abstract: The present invention provides an antenna for transmitting/receiving a first frequency signal and a second frequency signal. The antenna includes a first frequency antenna branch for transmitting/receiving the first frequency signal, a second frequency antenna branch separated from the first frequency antenna branch for transmitting/receiving the second frequency signal and an inductance for connecting the first and second frequency antenna branches in series with one another. The inductance is capable of preventing the first frequency signal passing through the second frequency antenna branch.

Abstract: A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.

Abstract: Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, flits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals.

Abstract: A microfluidic system with on-chip pumping which can be used for liquid chromatography and also electrospray ionization mass spectrometry and which provides improved efficiency, better integration with sensors, improved portability, reduced power consumption, and reduced cost.

Abstract: A mounting apparatus includes a drive bracket assembly, a back panel, and a securing member. The back panel is mounted to the drive bracket assembly along a first direction. The securing member is slidably mounted on the drive bracket assembly. The securing member secures the back panel to the drive bracket assembly to prevent the back panel moving along the first direction. The securing member is capable of sliding along a second direction that is generally perpendicular to the first direction to a position, so the back panel is capable of moving along the first direction.

Abstract: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.

Abstract: A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.