Abstract: The present invention relates to a CRISPR-Cas13 system and use thereof, and also relates to a Cas13 protein, a fusion protein, and a guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared to SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. The guide polynucleotide comprises a same-direction repetition sequence and a guide sequence that has been engineered to hybridize with the target RNA. The same-direction repetition sequence has at least 70% sequence identity to any of SEQ ID NOs: 3 and 80-87. The CRISPR-Cas13 system comprises the Cas13 protein that has at least 90% sequence identity to SEQ ID NO: 1, or a coding nucleic acid therefor, and the guide polynucleotide or a coding nucleic acid therefor.

Abstract: A data transmission method and apparatus, a terminal, a storage medium, and a system. The data transmission method includes: obtaining audio data and transmission status information, determining a compression factor and a redundancy factor based on the transmission status information, performing time domain data compression processing on the audio data according to the compression factor to obtain compressed data, performing channel coding on the compressed data according to the redundancy factor to obtain a data transmission packet, and transmitting the data transmission packet.

Abstract: An electronic device performs sub-band decomposition on a to-be-coded audio to obtain a to-be-coded low frequency signal corresponding to a low frequency band and a to-be-coded high frequency signal corresponding to a high frequency band. The device performs compression coding on the to-be-coded low frequency signal to obtain low frequency coded data of the to-be-coded low frequency signal. The device determines high frequency prediction information according to the to-be-coded low frequency signal. The device performs feature extraction on the to-be-coded high frequency signal to obtain high frequency feature information. The device determines high frequency compensation information of the to-be-coded high frequency signal according to a difference between the high frequency feature information and the high frequency prediction information.

Abstract: Provided are a novel Cas effector protein, a gene editing system, and uses thereof. The Cas effector protein is CasRfg.3 and similar proteins. The CasRfg.3 (also referred to as Ca2) protein of the present disclosure has a relatively shorter amino acid sequence compared to the commonly used SpCas9 protein, allowing it to be easily packaged into small-capacity gene therapy vectors. The CasRfg.3 corresponds to a unique PAM sequence. Additionally, the CasRfg.3 exhibits excellent specificity in targeting and editing nucleic acid sequences, shows good adaptation to temperature variations, and has a wide temperature tolerance range, functioning effectively under high-temperature conditions, such as being capable of cleaving or modifying target nucleic acids at temperatures ranging from 25° C. to 55° C. The novel Cas effector protein of the present disclosure has significant application value in fields like gene therapy.

Abstract: Embodiments of this application provide an audio data processing method performed by a computer device. The method includes the following steps: acquiring recorded audio; determining prototype audio matching a background reference audio component of the recorded audio from an audio database; extracting candidate speech audio from the recorded audio according to the prototype audio; determining a difference between the recorded audio and the candidate speech audio as the background reference audio component comprised in the recorded audio; performing environmental noise reduction on the candidate speech audio to obtain noise-reduced speech audio corresponding to the candidate speech audio; and combining the noise-reduced speech audio with the background reference audio component to obtain noise-reduced recorded audio.

Abstract: This disclosure provides a network call method and apparatus, a computer device, and a storage medium, and belongs to the field of audio data processing. The method includes: performing time-frequency transformation on an acquired audio signal, to obtain a plurality of pieces of frequency domain information of the audio signal; determining a target bit rate corresponding to the audio signal according to the plurality of pieces of frequency domain information; and encoding the audio signal based on the target bit rate, and performing a network call based on the encoded audio signal.

Abstract: A system, a method, and a composition for targeted gene manipulation and use thereof are provided. The system specifically targets an RNA molecule encoding an ApoE4 protein, which may be used to treat diseases such as Alzheimer's disease.

Abstract: The present invention relates to a CRISPR-Cas13 system and use thereof, and also relates to a Cas13 protein, a fusion protein, and a guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared to SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. The guide polynucleotide comprises a same-direction repetition sequence and a guide sequence that has been engineered to hybridize with the target RNA. The same-direction repetition sequence has at least 70% sequence identity to any of SEQ ID NOs: 3 and 80-87. The CRISPR-Cas13 system comprises the Cas13 protein that has at least 90% sequence identity to SEQ ID NO: 1, or a coding nucleic acid therefor, and the guide polynucleotide or a coding nucleic acid therefor.

Abstract: Provided are a nucleotide and a use thereof. Specifically provided is a nucleic acid molecule that specifically binds to exon 13 of USH2A pre-mRNA. Also provided is a preparation method for and use of the nucleic acid molecule. The nucleic acid molecule has the ability to specifically bind to sequence 3? of exon 13 of USH2A pre-mRNA or a fragment thereof, can perform targeted interference on Pre-mRNA splicing, and can increase the proportion of single skipping of exon 13 of USH2A pre-mRNA, so that safety can be ensured while efficiency is significantly improved.

Abstract: The present invention relates to a CRISPR-Cas13 system and use thereof, and also relates to a Cas13 protein, a fusion protein, and a guide polynucleotide. The Cas13 protein has at least 90% sequence identity compared to SEQ ID NO: 1. The fusion protein comprises the Cas13 protein fused to a protein domain and/or a polypeptide tag. The guide polynucleotide comprises a same-direction repetition sequence and a guide sequence that has been engineered to hybridize with the target RNA. The same-direction repetition sequence has at least 70% sequence identity to any of SEQ ID NOs: 3 and 80-87. The CRISPR-Cas13 system comprises the Cas13 protein that has at least 90% sequence identity to SEQ ID NO: 1, or a coding nucleic acid therefor, and the guide polynucleotide or a coding nucleic acid therefor.

Abstract: Provided is snRNA which targets USH2A pre-mRNA. The recognition domain of the snRNA is the reverse complement of a USH2A pre-mRNA sequence. The snRNA binds to the USH2A pre-mRNA to splice and jump exon 13. The snRNA promotes a higher exon 13 skipping efficiency than AON.

Abstract: The present disclosure relates to an isolated Cas13 protein and use thereof. The amino acid sequence of the isolated Cas13 protein comprises a sequence having ?50% sequence identity with the sequence as shown in any one of SEQ ID NO: 1-SEQ ID NO: 7, and SEQ ID NO: 60. The Cas13 protein is a Cas13 enzyme with an endonuclease activity, which can be used in a CRISPR/Cas system to achieve targeting and modification of a target nucleic acid, enriching the enzymes and systems available in a CRISPR-C as editing system.

Abstract: This application relates to a speech encoding method performed by a computer device the method, including: performing subband decomposition on a target speech signal to obtain a plurality of subband excitation signals; obtaining an auditory perception representational value that corresponds to each subband excitation signal; determining at least one first subband excitation signal and at least one second subband excitation signal from the at least two subband excitation signals; obtaining a gain of each of the at least one first subband excitation signal relative to a preset reference excitation signal as an encoding parameter that corresponds to the first subband excitation signal; obtaining a corresponding encoding parameter that is obtained by quantizing each of the at least one second subband excitation signal; and performing encoding on each subband excitation signal based on the encoding parameter corresponding to the subband excitation signal.

Abstract: This application provides a packet loss retransmission method, a computer-readable storage medium, and an electronic device. The packet loss retransmission method includes: obtaining a loudness corresponding to a target audio data packet; and in response to receiving a packet loss state indicating that the target audio data packet is lost, in accordance with a determination that the loudness corresponding to the target audio data packet meets a first threshold: retransmitting the target audio data packet. The technical solutions of this application may alleviate the problem of long data retransmission time, and improve data transmission efficiency.

Abstract: A call audio mixing processing method is provided. In the method, call audio streams from terminals of call members participating in a call are obtained. Voice analysis is performed on the call audio streams to determine voice activity corresponding to each of the terminals. The voice activity of the terminals indicate activity levels of the call members participating in the call. According to the voice activity of the terminals, respective voice adjustment parameters corresponding to the terminals are determined. According to the respective voice adjustment parameters corresponding to the terminals, the call audio streams of the terminals are adjusted. Further, mixing processing is performed on the adjusted call audio streams to obtain a mixed audio stream.

Abstract: A voice processing method is provided for a terminal. The method includes: performing voice speed detection on a voice obtained from a voice source, to obtain a voice speed value of the voice; obtaining a forward error correction (FEC) redundancy; adjusting the FEC redundancy according to the voice speed value to obtain a target redundancy; performing voice encoding on the voice to obtain a voice encoded packet; performing FEC encoding on the voice encoded packet according to the target redundancy to obtain a redundancy packet; and transmitting the redundancy packet and the voice encoded packet to a receiving end.

Abstract: Provided are an sgRNA targeting Aqp1 RNA, and a vector and use thereof. The sgRNA comprises a targeting structure and a backbone sequence, the targeting structure being complementary to a target nucleic acid Aqp1 RNA sequence, the backbone sequence being capable of binding to or interacting with a Cas protein. After the vector is used to deliver the sgRNA to cells, Aqp1 RNA is targeted and the Aqp1 RNA level is reduced, so as to treat diseases in which downregulation of Aqp1 expression is beneficial, including but not limited to diseases related to Aqp1 overexpression, including cancer and glaucoma.

Abstract: The present disclosure discloses a data transmission method performed by a computer device and a non-transitory computer-readable storage medium. According to the present disclosure, voice criticality analysis is performed on a to-be-transmitted audio to obtain a criticality level of each to-be-transmitted audio frame in the to-be-transmitted audio, and a corrected redundancy multiple of each to-be-transmitted audio frame is obtained according to a current redundancy multiple and a redundant transmission factor corresponding to the criticality level of each to-be-transmitted audio frame. Therefore, each to-be-transmitted audio frame is duplicated according to a corrected redundancy multiple of each to-be-transmitted audio frame, to obtain at least one redundancy data packet, and the at least one redundancy data packet is transmitted to a target terminal, which can improve the network anti-packet loss effect without causing network congestion.

Abstract: Provided is a new method for activating transcription of a gamma-globin gene. The method uses a single-stranded oligonucleotide (ssODN) containing GATA or an antisense complementary sequence TATC thereof as guidance information, and performs gene editing in a gamma-globin gene promoter region to form a GATA-containing enhancer element, which can promote the expression of the gamma-globin gene in mature red blood cells. Hematopoietic stem cells genetically edited by the method have normal functions, can significantly improve the expression of fetal hemoglobin after being differentiated into red blood cells, and therefore can be used in clinical treatment of beta-thalassemia and sickle cell anemia.

Abstract: Embodiments of this application provide an audio data processing method performed by a computer device. The method includes the following steps: acquiring recorded audio; determining prototype audio matching a background reference audio component of the recorded audio from an audio database; extracting candidate speech audio from the recorded audio according to the prototype audio; determining a difference between the recorded audio and the candidate speech audio as the background reference audio component comprised in the recorded audio; performing environmental noise reduction on the candidate speech audio to obtain noise-reduced speech audio corresponding to the candidate speech audio; and combining the noise-reduced speech audio with the background reference audio component to obtain noise-reduced recorded audio.