Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analog showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analog suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.

Abstract: Disclosed is a connection structure that connects a housing to a magnetic annealing furnace that is provided with a fixedly arranged magnet and includes a first drum flange having a cylindrical flange portion. The connection structure includes: a second drum flange that is attached and fixed to the housing and includes a flange portion configured to externally or internally fit the flange portion of the first drum flange thereto; a first sealant that is installed between facing surfaces of the housing and the second drum flange; and a second sealant that is installed between facing surfaces of the first and second drum flanges.

Abstract: Secreted luciferases which are different from those known heretofore have been desired. The present invention provides a luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from amino acids at the positions of 1 to 4 is deleted in the amino acid sequence of SEQ ID NO: 2.

Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.

Abstract: A crystal oscillator has characteristics in that reduction in oscillation margin makes the crystal oscillator less likely to oscillate, with the result that an amplitude of a voltage waveform at an input terminal of a CMOS inverter is reduced. A crystal oscillator of the invention has a detection circuit that outputs an alarm signal when the amplitude of the voltage waveform at the input terminal of the CMOS inverter becomes equal to or less than a predetermined specified value. As a result, it is possible to detect a change in the oscillation margin of the crystal oscillator without switching between circuits even after the crystal oscillator is incorporated in a device.

Abstract: A door locking device includes: a pivot end wall portion extending from a pivot end side of a door 131, and having a first through-hole formed therein; an opposed wall portion 20 being opposed to the pivot end wall portion when the door 131 is closed, and having a second through-hole 23A, 23B formed therein; a key cylinder including a movable element 33 configured to be displaced in accordance with unlocking/locking operation; and a pivot hook 40A, 40B configured to interlock with the movable element 33. When the door is unlocked, the pivot hook 40A, 40B retreats to an inner side of the door 131 with respect to the first through-hole, whereas when the door is locked, the pivot hook enters the second through-hole 23A, 23B via the first through-hole to keep the door 131 and a door 132 closed.

Abstract: A wristwatch including a case including a band attachment section having an attachment hole, a band including a coupling hole in one end portion located coaxially with the attachment hole of the band attachment section, a coupling member inserted into the coupling hole of the band and the attachment hole of the band attachment section and coupling the band with the band attachment section, and a fixing member fixing the coupling member to the attachment hole of the band attachment section or the coupling hole of the band.

Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.

Abstract: A dash silencer has a lamination structure of a first layer, an intermediate layer, and a second layer. The intermediate layer is formed as a non-air permeable thin-membrane layer. The second layer is formed as a perforated layer having a plurality of opening portions.

Abstract: A thermoelectric conversion module has a substrate, a plurality of first electrodes, a plurality of thermoelectric conversion elements 2, a plurality of second electrodes 4, and connectors 42. The plurality of thermoelectric conversion elements 2 are n-type elements, connected in series. The connector 42 is formed as a single unit with the second electrodes 4 and separate from the first electrodes 3. A receptor 33 that accepts the tip of the connector 42 is provided to each of the first electrodes 3. Six element rows 6 of five thermoelectric conversion elements 2 aligned along the X axis are arrayed along the Y axis. The receptors 33 are configured to accept connectors 42 of the same shape whether electrically connecting the thermoelectric conversion elements 2 within the element rows 6 or electrically connecting between adjacent element rows 6. The first electrodes 3 and second electrodes 4 are all the same shape.

Abstract: An excellent protein stabilizer is provided, which has the following effects: (1) low probability with contamination of pathogens, (2) the effect of stabilization on photoproteins, and (3) minimization of loss of activity under lyophilizing conditions. A peptide from fish is used as the active ingredient for the protein stabilizer.

Abstract: A structure includes a wristwatch case; an inner frame arranged inside the wristwatch case and having an upper surface provided with a positioning fitting recess; a timepiece module arranged as being positioned inside the inner frame; and a parting plate which is provided with a positioning pin that is provided on a lower surface of the parting plate facing the upper surface of the inner frame, and that is fitted into the positioning fitting recess of the inner frame, thereby positioning the parting plate with respect to respect to the inner frame. As a result, in a state where the timepiece module is arranged and positioned inside the inner frame arranged in the wristwatch case, the positioning pin of the parting plate can be fitted into the positioning fitting recess of the inner frame. Accordingly, the parting plate can be accurately positioned with respect to the inner frame.

Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.

Abstract: A direct-current interrupter in an embodiment includes a current-carrying path and a current-interrupting path connected in parallel to the current-carrying path. The current-carrying path includes a first switch and a second switch connected in series and a resistor connected in parallel to the second switch. The first switch has a predetermined first withstand voltage property and switches between conduction and non-conduction of current by using no semiconductor switch. The second switch has a second withstand voltage property lower in withstand voltage property than the first withstand voltage property and switches between conduction and non-conduction of current by using no semiconductor switch. The resistor protects the second switch from voltage to be applied to the second switch. The current-interrupting path includes a semiconductor switch and a current source connected in series and a non-linear resistor connected in parallel to a series connection of the semiconductor switch and the current source.

Abstract: An excellent protein stabilizer is provided, which has the following effects: (1) low probability with contamination of pathogens, (2) the effect of stabilization on photoproteins, and (3) minimization of loss of activity under lyophilizing conditions. A peptide from fish is used as the active ingredient for the protein stabilizer.

Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which tyrosine at position of 138 is substituted with another amino acid and at least 3 amino acids selected from the group consisting of isoleucine at position of 90, proline at position of 115, glutamine at position of 124 and asparagine at position of 166 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.

Abstract: A watch case having a through-hole, an operating member including an operating shaft to be inserted to the through-hole of the watch case, an exterior member mounted on the operating member and configured to be displaced in a shaft direction of the operating shaft and be rotated together with the operating member, and a buffer member located between the operating member and the exterior member are provided. Therefore, when the exterior member receives impact from outside, the impact can be buffered by the buffer member. As a result, the operating member can be prevented from being damaged because the impact from the outside is not directly transmitted to the operating member, thereby improving impact resistance.

Abstract: To improve the mass productivity of thermoelectric conversion modules. A thermoelectric conversion module 1 is equipped with a pair of substrates 11 and 12, a plurality of thermoelectric conversion elements 2, each having one end portion electrically connected to a first electrode 3 which is arranged on the substrate 11 and the other end portion electrically connected to a second electrode 4 which is arranged on the substrate 12, and a connection section 5 which electrically connects the first electrode 3 electrically connected to the thermoelectric conversion element 2 to the second electrode 4 electrically connected to an adjacent one of the thermoelectric conversion elements 2. The connection section 5 is separate from at least one of the first electrode 3 and the second electrode 4.

Abstract: Secreted luciferases which are different from those known heretofore have been desired. The present invention provides a luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from amino acids at the positions of 1 to 4 is deleted in the amino acid sequence of SEQ ID NO: 2.

Abstract: The radio wave receiver includes the metallic case body, the metallic rear case, and the antenna inside the case body. The screw portion is formed in the metal wall of the case body. The coupling resin member having an electrical insulating property is coupled to the rear case through a great number of fine irregularities formed in the metal wall thereof. The screw portion which engages with the screw portion is formed on the coupling resin member. The case body and the rear case are coupled to each other with the screw portions. The case body and the rear case are electrically insulated from each other by the electrical insulating function of the coupling resin member so as to improve receiving sensitivity of the antenna.