Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

Abstract: Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

Abstract: Disclosed herein are systems and methods for providing targeted temperature management (TTM) therapy to a patient. For example, TTM systems can include rotatable connector systems for coupling a fluid deliver line to a thermal contact pad. Embodiments of the connector system can include connector pairs that comprise one lumen or multiple lumens. The TTM system includes embodiments where a second thermal pad is coupled to a first thermal pad.

Abstract: Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.

Abstract: A flight display is described. The flight display includes a display screen, a physical network interface, and multiple processors. The flight display interfaces with the flight deck by way of the physical network interface. The display screen displays images representative of avionics data in response to signals generated by one or more of the processors. The processors also generate command signals causing an actuator of the aircraft to control a flight control surface or a throttle. In this regard, the one or more of the processors perform various automatic flight control system (AFCS) functions. A system is further described including multiple of the flight displays. By hosting the AFCS functions across processors of the multiple flight displays, a level of command integrity may be reached. Furthermore, backups flight displays are available to be reconfigured to perform the AFCS functions.

Abstract: System and method for benchmarking a container orchestration platform in a computing environment uses data contained in a configuration file, which specifies at least one benchmark operation having benchmark actions for container orchestration resource types to be performed in the container orchestration platform, to create a resource manager for each container orchestration resource type specified in the configuration file. Using the created resource managers, a routine for each benchmark action is spawned and executed on container orchestration objects in the container orchestration platform. As the routines are executed, performance metrics are collected and at least some of the collected performance metrics are displayed.

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

Abstract: A sealing member for providing a seal between fluid connectors includes a tubular member defining a lumen extending between a first end and a second end. The tubular member may receive a first connector via the first end and a second connector via the second end, and includes an annular wall extending between a first annular portion adjacent the first end and a second annular portion adjacent the second end. The first annular portion may engage the first connector, and the second annular portion may engage the second connector. When a pressure within the lumen is negative, atmospheric acting inward on the annular wall compresses the sealing member to define a contact force between the second annular portion and the second connector sufficient to define a fluid seal between the sealing member and the second connector.

Abstract: A heterogeneous memory system is implemented using a low-latency near memory (NM) and a high-latency far memory (FM). Pages in the memory system include NM blocks stored in the NM and FM blocks stored in the FM. A page is assigned to a region in the memory system based on the proportion of NM blocks in the page. When accessing a block, the block address is used to determine a region of the memory system, and a block offset is used to determine whether the block is stored in NM or FM. The memory system may observe memory accesses to determine the access statistics of the page and the block. Based on a page's hotness and access density, the page may be migrated to a different region. Based on a block's hotness, the block may be migrated between NM and FM allocated to the page.

Abstract: Provided are methods for detecting specific nucleotide sequences in samples. Methods include generating, from the specific nucleotide sequences, nucleic acid constructs containing probe-identification sequences and sample identification sequences, pooling the nucleic acid constructs from the samples into a single combined sample, and determining the abundance of the specific nucleotide sequences in the samples by quantifying the probe-identification sequences and sample-identification sequences of the nucleic acid constructs.

Abstract: Disclosed herein are systems, pads, and methods thereof for targeted temperature management. In an example, a pad for targeted temperature management can include a pad body, a conduit system, one or more inlets, and one or more outlets. The pad body can be of a conformable material configured to conform to a body of a patient on the pad. The conduit system can be disposed in the pad body or an overlayer configured for placement over the pad body. The conduit system can include one or more conduits configured to convey a fluid through the pad body or the overlayer. The one-or-more inlets can be configured for charging the conduit system with the fluid, while the one-or-more outlets can be configured for discharging the fluid from the conduit system. Methods of the systems and pads can include methods of use. In another example, a deformable pad can be configured for receiving a neonatal patient.

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

Abstract: A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.

Abstract: System and method for benchmarking a container orchestration platform in a computing environment uses data contained in a configuration file, which specifies at least one benchmark operation having benchmark actions for container orchestration resource types to be performed in the container orchestration platform, to create a resource manager for each container orchestration resource type specified in the configuration file. Using the created resource managers, a routine for each benchmark action is spawned and executed on container orchestration objects in the container orchestration platform. As the routines are executed, performance metrics are collected and at least some of the collected performance metrics are displayed.

Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.