Abstract: Biomedical devices and methods are disclosed for the development of 3D polymeric scaffolds for cell culture, high throughput screens for biomolecule purification, and generation of bone mimetic materials. The devices may feature multiple geometries, and scaffold generation capabilities include multiple gel types utilizing organic and aqueous phase pregel. Additionally, macroporous and non-macroporous morphologies are possible.

Abstract: A method to form a lipid-containing aminoglycoside-based polymer, where the method includes reacting an aminoglycoside with a diepoxide to form an aminoglycoside-based polymeric material, and then reacting the aminoglycoside-based polymeric material with an acyl chloride to form the lipid-containing aminoglycoside-based polymer.

Abstract: A tissue closure device can include a structural material and a stimulus responsive material on or in the structural material. The structural material can be biodegradable and/or bioabsorbable (e.g., biocompatible natural and/or semi-natural and/or synthetic polymer). The stimulus responsive material can be a particle, such as a nanoparticle. The structural material is shaped as a suture, staple, screw, patch, adhesive, sealant, or the like. A biologically active agent can be included. A method of promoting wound healing can include: approximating tissue portions; and stimulating the stimulus responsive material with a stimulus to cause the tissue portions of the wound to adhere to each other. The stimulus is selected from optical, electrical, thermal, chemical, mechanical, magnetic, acoustic, pressure, shear, biological, or enzymatic sources.

Abstract: An apparatus includes a solution including a metallic compound, a surfactant, and an acid. The solution is substantially colorless. A container holds the solution. A radiated solution is formed when the solution receives a low dose of ionizing radiation.

Abstract: Methods to form a novel aminoglycoside based hydrogel for high-throughput generation of 3D dormant, relapsed and micrometastatic tumor microenvironments are disclosed. In addition, methods of screening agents against tumor cells grown in the 3D environments disclosed herein that include, for example, screening of lead drugs and therapies for an effect on dormant, relapsed and/or micrometastatic tumor cells.

Abstract: A method to form a lipid-containing aminoglycoside-based polymer, where the method includes reacting an aminoglycoside with a diepoxide to form an aminoglycoside-based polymeric material, and then reacting the aminoglycoside-based polymeric material with an acyl chloride to form the lipid-containing aminoglycoside-based polymer.

Abstract: Biomedical devices and methods are disclosed for the development of 3D polymeric scaffolds for cell culture, high throughput screens for biomolecule purification, and generation of bone mimetic materials. The devices may feature multiple geometries, and scaffold generation capabilities include multiple gel types utilizing organic and aqueous phase pregel. Additionally, macroporous and non-macroporous morphologies are possible.

Abstract: Disclosed herein are methods of connecting disrupted tissue, tissue repair, treating colorectal disorder and tissue welding. The methods comprise using a bioadhesive composition comprising ELP and light absorbing chromophores and irradiating the bioadhesive tissue.

Abstract: Disclosed herein are polymers that can be made cationic and used to deliver a substance into a cell. Also disclosed are pharmaceutical compositions comprising the polymers and methods of using the polymers.

Abstract: Aminoglycoside-polyamines are disclosed along with methods of use thereof in displacement chromatography and as DNA-binding ligands. The aminoglycoside-polyamines are derivatives of carbohydrates, such as sugars, amino sugars, deoxysugars, glycosides, nucleosides and their substituted counterparts. The subject polyamines possess a group in place of at least one hydrogen atom of at least one hydroxyl group of the carbohydrate compound. In these compounds R1 is an alkyl group or an azaalkyl group, and R2 is a primary or secondary amino group.

Abstract: Aminoglycoside-polyamines are disclosed along with methods of use thereof in displacement chromatography and as DNA-binding ligands. The aminoglycoside-polyamines are derivatives of carbohydrates, such as sugars, amino sugars, deoxysugars, glycosides, nucleosides and their substituted counterparts. The subject polyamines possess a group in place of at least one hydrogen atom of at least one hydroxyl group of the carbohydrate compound. In these compounds R1 is an alkyl group or an azaalkyl group, and R2 is a primary or secondary amino group.

Abstract: A bioproduct may be selectively separated from one or more impurities by means of a displacement chromatography system that includes a solvent, a chromatographic resin and a chemically selective displacer. The method includes: dissolving the bioproduct and the one or more impurities in a solvent; loading the bioproduct and the one or more impurities, in the solvent, on a chromatographic resin; displacing the bioproduct from the chromatographic resin with chemically selective displacer; and retaining the one or more impurities on the chromatographic resin. For this method, the bioproduct and the impurities have similar binding affinity for the chromatographic resin in the absence of the displacer.

Abstract: An effective technique for the high throughput screening of displacers is described. In this technique, potential displacers are employed to displace a biomolecule (e.g., protein) adsorbed on a chromatographic resin in small-scale batch displacement experiments. The amount of protein displaced from a specific resin by a defined concentration of displacer is determined by monitoring the supermatant for the protein. By evaluating the displaced protein rather than the displacer itself, this technique enables a single detection technique (e.g., absorbance, fluorescence, etc.) to be employed for all batch displacement experiments. By monitoring the amount of protein displaced, the effacy of a large number of potential displacers can be rapidly evaluated. The entire experimental procedure can be carried out rapidly and is thus amenable to high throughput parallel screening of molecules possessing a large range of affinities and physico-chemical properties.

Abstract: An effective technique for the high throughput screening of displacers is described. In this technique, potential displacers are employed to displace a biomolecule (e.g., protein) adsorbed on a chromatographic resin in small-scale batch displacement experiments. The amount of protein displaced from a specific resin by a defined concentration of displacer is determined by monitoring the supermatant for the protein. By evaluating the displaced protein rather than the displacer itself, this technique enables a single detection technique (e.g., absorbance, fluorescence, etc.) to be employed for all batch displacement experiments. By monitoring the amount of protein displaced, the effacy of a large number of potential displacers can be rapidly evaluated. The entire experimental procedure can be carried out rapidly and is thus amenable to high throughput parallel screening of molecules possessing a large range of affinities and physico-chemical properties.