Abstract: A specific amount of a processing solution is supplied on a wafer by spraying the processing solution from a first end (tip) of a nozzle. The solution surface of the processing solution remaining in the nozzle is sucked back to a second end side of the nozzle by aspirating the remaining processing solution to the second end side. The first end of the nozzle is then soaked into a fluid. The processing solution remaining in the nozzle is aspirated to the second end side to aspirate a specific amount of the fluid into the first end of the nozzle for further sucking back the solution surface of the processing solution to the second end side, thus the solution surface of the processing solution being not touching the fluid.

Abstract: A specific amount of a processing solution is supplied on a wafer by spraying the processing solution from a first end (tip) of a nozzle. The solution surface of the processing solution remaining in the nozzle is sucked back to a second end side of the nozzle by aspirating the remaining processing solution to the second end side. The first end of the nozzle is then soaked into a fluid. The processing solution remaining in the nozzle is aspirated to the second end side to aspirate a specific amount of the fluid into the first end of the nozzle for further sucking back the solution surface of the processing solution to the second end side, thus the solution surface of the processing solution being not touching the fluid.

Abstract: The instant invention relates to a method for culturing and floating animal cells in a double-bag container, where an outer bag containing culture media and a volume of air houses an inner bag of a semipermeable film containing cells suspended in a culture media. The semipermeable film has pores of sufficient size so as to prohibit cells within the inner bag from passing therethrough but allowing culture liquid and air to pass through it. To optimize the exchange of nutrients between the interiors of the two bags, a protective mesh surrounds the inner bag and the double-bag container is fastened to an agitator which provides either a rotating or shaking motion.

Abstract: The instant invention relates to an apparatus for floating animal cells in a double-bag container, where an outer bag containing culture media and a volume of air houses an inner bag of a semipermeable film containing cells suspended in a culture media. The semipermeable film has pores of sufficient size so as to prohibit cells within the inner bag from passing therethrough but allowing culture liquid and air to pass through it. To optimize the exchange of nutrients between the interiors of the two bags, a protective mesh surrounds the inner bag and the double-bag container is fastened to an agitator which provides either a rotating or shaking motion.