Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Abstract: A method of measuring a lipid in a specific lipoprotein characterized by using a polycyclic polyoxyalkylene derivative at least in the step of determining the specificity of the measurement of the target lipid.

Abstract: A bacterium belonging to the genus Escherichia and having an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing an expression amount of an L-amino acid excretion protein, and a method for producing the L-amino acid using the bacterium.

Abstract: A bacterium belonging to the genus Escherichia which has an ability to produce an L-amino acid, wherein the ability to produce the L-amino acid is increased by increasing expression of an L-amino acid excretion protein is described. A method for producing the L-amino acid using the bacterium is also described.

Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Abstract: In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and accumulate the target substance in the medium and collecting the target substance, there is used, as the microorganism, a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics:

Abstract: Threonine or isoleucine is be produced by culturing a bacterium belonging to the genus Escherichia, which has an ability to produce L-threonine or L-isoleucine, and in which intracellular phosphoenolpyruvate carboxylase activity and transhydrogenase activity are enhanced, in a medium to produce and accumulate threonine or isoleucine in the medium, and collecting the threonine or isoleucine from the medium.

Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density—and very low density—cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Abstract: A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Abstract: A DNA encoding aspartokinase III derived from bacteria of the genus Escherichia and having mutation by which feedback inhibition with L-lysine is released is introduced into cells to form transformant bacteria of the genus Escherichia. These bacteria are incubated in an appropriate culture medium. An L-amino acid is produced and accumulated in the culture, and collected from this culture.

Abstract: Disclosed is a concrete-filled steel bearing earthquake resistant wall which shows combined resistance to shearing forces exerted in the surface plane of an earthquake resistant wall. The wall comprises connecting members 1, a pair of surface steel plates 2 and 2' which are arranged in a parallel relationship to each other and secured by the connecting members 1 so that each space between these surface steel plates 2 is filled with the wall unit concrete 7, and wall unit periphery binding steel reinforcements 8 constructed from inverted U-shaped steel bars or similar material and arranged along the peripheral part of the wall unit concrete 7 at predetermined intervals so as to be fixed to said concrete 7. In addition, the slippage preventing members 6 are arranged in a staggered arrangement on the aforementioned surface steel plates 2 and 2'.

Abstract: A concrete-filled steel bearing earthquake resistant wall which has combined resistance to shearing forces exerted in the surface plane thereof has connecting members (1), a pair of surface steel plates (2 and 2') arranged in parallel spaced relationship to each other and secured by the connecting members (1) so that the space between these surface steel plates is filled with the wall unit concrete (7), and wall unit periphery binding steel reinforcements (8) constructed from inverted U-shaped steel bars or similar material and arranged along the peripheral part of the wall unit at predetermined intervals so as to be embedded in the concrete (7). Slippage preventing members (6) are arranged in a staggered arrangement on the aforementioned surface steel plates (2 and 2').

Abstract: The present invention relates to a DNA fragment having a gene derived from fish which codes for a polypeptide possessing transglutaminase activity, a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase, a transformant into which a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase is introduced, and a method for the production of a transglutaminase, comprising culturing a transformant containing a fish-derived DNA fragment which codes for a transglutaminase.

Abstract: The present invention relates to a DNA fragment having a gene derived from fish which codes for a polypeptide possessing transglutaminase activity, a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase, a transformant into which a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase is introduced, and a method for the production of a transglutaminase, comprising culturing a transformant containing a fish-derived DNA fragment which codes for a transglutaminase.

Abstract: A package transfer system having a package elevator located between an overhead conveyor and stock conveyors. Withdrawable delivery members are installed on the runway of the package elevator. When in an up position, the delivery members function to deliver packages from the descending elevator to the stock conveyor. When in a down position, the delivery members allow the downward passage of the packages on the elevator.

Abstract: A package storing apparatus wherein a belt conveyor is provided adjacent to a package storehouse provided with a number of placing shelves on which a package erected on a tray is placed. A package transfer device for transferring a package between the package storehouse and the belt conveyor comprises a fluid-pressure actuator arranged in a vertical direction, a rack connected with the fluid-pressure actuator and reciprocated thereby, a transfer sack connected with the sack by a driving force transmitting device, and a hook for holding the tray, which is provided on a support rod secured to the transfer sack.

Abstract: A package stock device in which a plurality of package mounting shelves are arranged within a case through endless chain in such a way as they may be circulated and moved in it, and the case is formed with a supplying port and a discharging port so as to supply the packages onto the mounting shelves and discharge the packages from the shelves. The package stock device may be provided with a steam supplying means.

Abstract: A bobbin transporting system includes a spinning bobbin transporting path and an empty bobbin transporting path both provided between a spinning frame and a winder, and a spinning bobbin reservoir path is formed intermittently of the spinning bobbin transporting path between the spinning frame and winder for temporarily reserving thereon all of spinning bobbins on a transport band which have been doffed by the spinning frame.