Abstract: The object of the present invention is to provide a method for screening particles such as cells efficiently. The object can be solved by a method for detecting a particle in droplet by illuminating light with one direction onto the particle which is contained in water in oil while it is flowing together with the oil in a flow path and detecting changes in the intensity of the light signal from the particles over time, characterized in that the particle in the droplet is detected by measuring the scattered light of the droplet and the scattered light of the particle.

Abstract: The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-likesubstance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.

Abstract: The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration, (4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur.

Abstract: The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-like substance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.

Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.

Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.

Abstract: An apparatus for analyzing particles in a solution includes a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.

Abstract: There is provided a multistage centrifugal fluid machine capable of improving efficiency of an operation for assembling an inner casing to a high pressure side head flange. The multistage centrifugal fluid machine 100 includes a rotor 3 provided with a plurality of impellers (41 to 81) in an axial direction, a cylindrical outer casing 2, and an inner bundle 1 that is fitted with the outer casing 2 to form a flow passage for the working fluid. The inner bundle 1 includes a high pressure side head flange 11, a low pressure side head flange 12, and an inner casing 5 disposed between the high pressure side head flange 11 and the low pressure side head flange 12. The high pressure side head flange 11 and the inner casing 5 are fastened with a first bolt 142 via an elastic body 144.

Abstract: The object of the present invention is to provide an evaluation method capable of accurately determining a metastasis of cancer, the stage of cancer progression, or the malignancy of cancer.

Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

Abstract: The present invention provides an apparatus for analyzing particles in a solution including a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.

Abstract: The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration, (4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur.

Abstract: There is provided a multistage centrifugal fluid machine capable of improving efficiency of an operation for assembling an inner casing to a high pressure side head flange. The multistage centrifugal fluid machine 100 includes a rotor 3 provided with a plurality of impellers (41 to 81) in an axial direction, a cylindrical outer casing 2, and an inner bundle 1 that is fitted with the outer casing 2 to form a flow passage for the working fluid. The inner bundle 1 includes a high pressure side head flange 11, a low pressure side head flange 12, and an inner casing 5 disposed between the high pressure side head flange 11 and the low pressure side head flange 12. The high pressure side head flange 11 and the inner casing 5 are fastened with a first bolt 142 via an elastic body 144.

Abstract: A disposable flow cell formed on a substrate for separating particles from a sample solution includes a sample flow path and a sample reservoir formed on the substrate, wherein the sample flow path is connected to the sample reservoir for introducing the sample solution from the sample reservoir, the sample flow path comprises an illuminating region configured for illumination by an external illumination unit, wherein the sample reservoir is adapted to be airtightly connected to an external constant air pump such that a constant air pressure can be applied to the sample solution by the external constant air pump; and a first branched flow path and a second branched flow path perpendicularly connected, respectively, to both sides of the sample flow path at a location downstream of the illumination region, wherein the first branched flow path comprises a connection port configured for air-tight connection to an external pulse air pump.

Abstract: The object of the present invention is to provide (1) a cell sorter, (2) a flow cytometer capable of detecting sideward scattered light, (3) a method for accurately measuring cell concentration, (4) a method for multicolor staining analysis without a fluorescence correction, and the like, which satisfy requirements that carry-over and cross contamination of samples do not occur.

Abstract: A method for sorting a particle of interest in a solution includes storing a sample solution in a sample solution reservoir and applying a constant pressure to the sample solution; illuminating the sample solution flowing in an illumination region of the sample solution flow path upstream of a branched flow path region, and detecting the particle of interest contained in the sample solution; and upon detecting the particle of interest, opening an electromagnetic valve or electromagnetic valves that are disposed between an air pressure pump and the branched flow path region of the sample solution flow path at a timing that the detected particle of interest reaches the branched flow path region such that a pulse pressure is applied to the branched flow path region to separate the detected particle of interest from the sample solution flow path.

Abstract: The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-like substance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.

Abstract: An apparatus for analyzing particles in a solution includes a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.