Abstract: Applying an ultrasound to a sample solution by an ultrasound application unit and pressing a fluorescent labeling substance in the sample solution against a metal layer. Under this state, applying an excitation light to the interface between a dielectric plate and the metal layer at a specific angle greater than or equal to a total reflection angle by a light application unit and forming a surface plasmon enhanced optical field area on the metal layer, thereby exciting the fluorescent labeling substance. Detecting fluorescence generated from the fluorescent labeling substance through the excitation by a fluorescence detection unit.

Abstract: Expedient and sensitive detection of a detection target substance is enabled in target substance detection that detects the detection target substance at a sensor portion provided within a flow channel. A detection target substance detecting method for a detecting detection target substance which may be contained in a liquid sample emits ultrasonic waves that propagate in a direction transverse to a flow channel when the liquid sample flows within a ultrasonic wave emission region at which ultrasonic waves can be emitted, to concentrate the detection target substance at a wall surface of the flow channel toward the side of the sensor portion and to detect the detection target substance.

Abstract: A sensor chip includes a dielectric plate and a sensor portion having a metal layer deposited on a predetermined area on the dielectric plate. A photo-reactable labeling-substance of an amount corresponding to the amount of a substance to be detected in a sample binds to the sensor portion by contacting the sample with the sensor portion. The amount of the substance to be detected is obtained by irradiating the predetermined area with excitation light and by detecting light output from the photo-reactable labeling-substance in an enhanced electric field that has been generated on the metal layer by irradiation with the excitation light. The photo-reactable labeling-substance includes a photo-reactable substance enclosed by a light transmissive material that transmits light output from the photo-reactable substance to prevent metal quenching that occurs when the photo-reactable substance is located close to the metal layer.

Abstract: An analyzer includes a holder for a chip having a spreading layer for a sample including a biological material and a reaction layer with a reagent that reacts with the biological material and generates a chromogenic material, a first light source that applies first light that is absorbed by the chromogenic material, a first detector that detects first output light from the chip irradiated with the first light, a second light source that applies second light that is absorbed by the biological material, a second detector that detects second output light from the chip irradiated with the second light, and a calculation unit that calculates a first concentration of the biological material from the first output light, calculates a spreading state of the sample from the second output light, and calculates a second concentration of the biological material by correcting the first concentration using the calculated spreading state.

Abstract: In a detection method for detecting the quantity of a target material, a labeled binding material in the amount corresponding to the quantity of the target material contained in a liquid specimen is bonded to the top of a sensor portion; and a signal based on light emitted from a label in an evanescent field or an enhanced optical field produced on a surface of the sensor portion when the sensor portion is irradiated with excitation light is detected. After the labeled binding material is bonded to the immobilization layer, the signal is detected while the fluid over the sensor portion is controlled to flow at a constant flow rate at which bonds between the labeled binding material and the immobilization layer are not broken and the above signal can be detected with a greater magnitude than when the liquid specimen exists over the sensor portion at rest.

Abstract: A target substance detecting method employs a detecting chip equipped with a fine flow channel. Examinations, in which clogging of the flow channel and inhibition of immune reactions can be easily and expediently prevented without preliminary processes, are enabled to be performed without fluctuations in hemolysis rates. The detecting chip having a flow channel base having the flow channel and a detecting portion formed in the flow channel is employed. A liquid sample that may contain a target substance and contains cellular non target substances is caused to flow into the flow channel. An ultrasonic wave emitting section provided upstream of the detecting portion emits ultrasonic waves into the liquid sample from a direction perpendicular to the longitudinal direction of the flow channel such that a standing wave is generated within the flow channel.

Abstract: A biological substance detecting apparatus that enables stable detection and quantitative analysis of biologically derived target substances is provided. The biological substance detecting apparatus employs a sensor chip provided with a fine flow channel through which a sample liquid is caused to flow, and a sensor surface that adsorbs a biologically derived target substance. Light is irradiated onto the sensor surface, and light emitted by the adsorbed target substance or a labeling substance bound thereto is detected. A flow speed controlling means, for controlling the flow speed of the sample liquid that flows through the fine flow channel to a value within a range such that the adsorption speed of the target substance is not controlled by dispersion.

Abstract: Detection of detection target substances at a sensor portion is expedited and the efficiency thereof is improved in biological substance analysis, to enable accelerated analysis with high sensitivity. A biological substance analyzing cell equipped with a reaction chamber having a sample supply space, an acoustic matching layer which is provided at a predetermined region of an inner wall of the reaction chamber that faces another inner wall, and a sensor portion provided within the reaction chamber is employed. Ultrasonic waves are emitted such that a standing wave are generated between the acoustic matching layer and the inner wall of the reaction chamber that faces the acoustic matching layer. The detection target substance is concentrated by the capturing forces of the standing waves, and the concentrated detection target substance is detected at the sensor portion.

Abstract: The sensing apparatus includes a measuring light emission device, a waveguide member including a sensing surface modified by a surface modification substance, a detection device and an analyzer. The measuring light of a predetermined polarized state is emitted from the emission device so that the measuring light is totally reflected on the sensing surface of the waveguide member holding target substances labeled by fine metal particles to illuminate the particles by evanescent light generated near the sensing surface. The amount of evanescent light scattered by the fine metal particles is detected by the detection device. The measuring light emission device, the waveguide member and the analyzer are included in an optical waveguide system which sets a polarized state of scattered light generated when no target substance is present on the sensing surface in a crossed nicol relation to the analyzer.

Abstract: A labeling binding substance in an amount corresponding to the amount of a detection target substance contained in a liquid sample binds to a sensor portion, and the amount of the detection target substance is detected based on the amount of signal light output by excitation of a label of the labeling binding substance in an enhanced optical field on the sensor portion. In this detection method, a labeling substance that includes a light-responsive substance enclosed by a dielectric that transmits light output from the light-responsive substance is used as the label, and the labeling binding substance binds to the sensor portion through a plurality of fragmented antibodies.

Abstract: A sensor chip including a sensor portion that has at least a metal layer deposited on a surface of a dielectric plate is used. A fluorescent-label binding-substance in an amount corresponding to the amount of a detection target substance in a liquid sample binds onto the sensor-portion. The amount of the detection target substance is detected based on the amount of light generated by excitation of a fluorescent label in the fluorescent-label binding substance. An electrified fluorescent substance containing a plurality of fluorescent dye molecules enclosed by a material that transmits fluorescence output from the plurality of fluorescent dye molecules is used as the fluorescent label. The electrified fluorescent substance is attracted to the sensor portion by applying voltage to the liquid sample in a state in which the fluorescent-label binding substance has bound to the sensor portion. In this state, the amount of the detection target substance is detected.

Abstract: A sensor chip includes a dielectric plate and a sensor portion having a metal layer deposited on a predetermined area on the dielectric plate. A photo-reactable labeling-substance of an amount corresponding to the amount of a substance to be detected in a sample binds to the sensor portion by contacting the sample with the sensor portion. The amount of the substance to be detected is obtained by irradiating the predetermined area with excitation light and by detecting light output from the photo-reactable labeling-substance in an enhanced electric field that has been generated on the metal layer by irradiation with the excitation light. The photo-reactable labeling-substance includes a photo-reactable substance enclosed by a light transmissive material that transmits light output from the photo-reactable substance to prevent metal quenching that occurs when the photo-reactable substance is located close to the metal layer.

Abstract: The sensing apparatus includes a measuring light emission device, a waveguide member including a sensing surface modified by a surface modification substance, a detection device and an analyzer. The measuring light of a predetermined polarized state is emitted from the emission device so that the measuring light is totally reflected on the sensing surface of the waveguide member holding target substances labeled by fine metal particles to illuminate the particles by evanescent light generated near the sensing surface. The amount of evanescent light scattered by the fine metal particles is detected by the detection device. The measuring light emission device, the waveguide member and the analyzer are included in an optical waveguide system which sets a polarized state of scattered light generated when no target substance is present on the sensing surface in a crossed nicol relation to the analyzer.