Patents by Inventor Kazuyoshi Yajima
Kazuyoshi Yajima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180271930Abstract: A method for raising poultry includes feeding poultry with a feed comprising oxidized glutathione during a period between hatching and 24 to 168 hours after the hatching.Type: ApplicationFiled: June 8, 2018Publication date: September 27, 2018Applicant: Kaneka CorporationInventors: Kazuyoshi Yajima, Kan Sato
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Patent number: 9926580Abstract: A process for producing on an industrial scale the oxidized coenzyme Q10, includes culturing a reduced coenzyme Q10-producing microorganism selected from the group consisting of the genus Rhodobacter, the genus Saitoella, the genus Schizosaccharomyces and the genus Trichosporon, to obtain microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10; and one of: (a) oxidizing thus-obtained reduced coenzyme Q10 to oxidized coenzyme Q10 and then extracting the oxidized coenzyme Q10 by an organic solvent; or (b) extracting reduced coenzyme Q10 by an organic solvent and oxidizing the extracted reduced coenzyme Q10 to oxidized coenzyme Q10.Type: GrantFiled: March 21, 2016Date of Patent: March 27, 2018Assignee: KANEKA CORPORATIONInventors: Kazuyoshi Yajima, Takahisa Kato, Akihisa Kanda, Shiro Kitamura, Yasuyoshi Ueda
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Publication number: 20160304915Abstract: A process for producing on an industrial scale the oxidized coenzyme Q10, includes culturing a reduced coenzyme Q10-producing microorganism selected from the group consisting of the genus Rhodobacter, the genus Saitoella, the genus Schizosaccharomyces and the genus Trichosporon, to obtain microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10; and one of: (a) oxidizing thus-obtained reduced coenzyme Q10 to oxidized coenzyme Q10 and then extracting the oxidized coenzyme Q10 by an organic solvent; or (b) extracting reduced coenzyme Q10 by an organic solvent and oxidizing the extracted reduced coenzyme Q10 to oxidized coenzyme Q10.Type: ApplicationFiled: March 21, 2016Publication date: October 20, 2016Inventors: Kazuyoshi Yajima, Takahisa Kato, Akihisa Kanda, Shiro Kitamura, Yasuyoshi Ueda
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Patent number: 9315839Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.Type: GrantFiled: February 3, 2011Date of Patent: April 19, 2016Assignee: KANEKA CORPORATIONInventors: Kazuyoshi Yajima, Takahisa Kato, Akihisa Kanda, Shiro Kitamura, Yasuyoshi Ueda
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Patent number: 8889389Abstract: The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.Type: GrantFiled: October 29, 2013Date of Patent: November 18, 2014Assignee: Kaneka CorporationInventors: Akihiko Kosugi, Kazuyoshi Yajima
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Publication number: 20140080179Abstract: The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.Type: ApplicationFiled: October 29, 2013Publication date: March 20, 2014Applicant: Kaneka CorporationInventors: Akihiko KOSUGI, Kazuyoshi YAJIMA
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Patent number: 8597908Abstract: The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.Type: GrantFiled: July 1, 2005Date of Patent: December 3, 2013Assignee: Kaneka CorporationInventors: Akihiko Kosugi, Kazuyoshi Yajima
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Patent number: 8163525Abstract: The present disclosure describes a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present disclosure also describes a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a cell of a host organism.Type: GrantFiled: June 13, 2008Date of Patent: April 24, 2012Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
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Publication number: 20110136191Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.Type: ApplicationFiled: February 3, 2011Publication date: June 9, 2011Applicant: KANEKA CORPORATIONInventors: Kazuyoshi YAJIMA, Takahisa KATO, Akihisa KANDA, Shiro KITAMURA, Yasuyoshi UEDA
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Patent number: 7910340Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.Type: GrantFiled: October 31, 2007Date of Patent: March 22, 2011Assignee: Kaneka CorporationInventors: Kazuyoshi Yajima, Takahisa Kato, Akihisa Kanda, Shiro Kitamura, Yasuyoshi Ueda
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Publication number: 20090130727Abstract: The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present invention also relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.Type: ApplicationFiled: June 13, 2008Publication date: May 21, 2009Inventors: Hideyuki MATSUDA, Makoto KAWAMUKAI, Kazuyoshi YAJIMA
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Patent number: 7402413Abstract: The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism. The present invention relates to a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a host microorganism.Type: GrantFiled: May 10, 2002Date of Patent: July 22, 2008Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
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Publication number: 20080171373Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.Type: ApplicationFiled: October 31, 2007Publication date: July 17, 2008Inventors: Kazuyoshi Yajima, Takahisa Kato, Akihisa Kanda, Shiro Kitamura, Yasuyoshi Ueda
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Publication number: 20080064074Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Rhodotorula. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1, 3 or 5 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.Type: ApplicationFiled: August 10, 2007Publication date: March 13, 2008Applicant: KANEKA CORPORATIONInventors: Hideyuki MATSUDA, Makoto KAWAMUKAI, Kazuyoshi YAJIMA, Yasuhiro IKENAKA
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Patent number: 7320883Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity.Type: GrantFiled: December 27, 2001Date of Patent: January 22, 2008Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka
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Publication number: 20070243582Abstract: The present invention relates to an efficient and economical process for producing a protein A-like protein. Hosts such as Escherichia coli and Bacillus subtilis have been used in the production of a protein A-like protein using a genetic recombination technique and however, their low productivity has been a big cause of high cost. Thus, it has been desired strongly to immediately establish a technique enabling the inexpensive, large-scale production of a protein A-like protein using recombinant DNA techniques other than Escherichia coli and Bacillus subtilis. The present invention provides a process for producing a protein A-like protein in large amounts, for example, a process comprising allowing a recombinant Brevibacillus genus bacterium to express and secrete the protein in large amounts into a culture solution and separating and collecting the accumulated protein A-like protein from the culture solution.Type: ApplicationFiled: July 1, 2005Publication date: October 18, 2007Applicant: KANEKA CORPORATIONInventors: Akihiko Kosugi, Kazuyoshi Yajima
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Patent number: 7195892Abstract: The subject of the present invention is to provide a ?-lactam acylase protein having high activity, a gene coding for said ?-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a ?-lactam antibiotic such as amoxycillin using said ?-lactam acylase. A ?-lactam acylase gene of Stenotrophomonas maltophilia was cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and a Stenotrophomonas ?-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having ?-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.Type: GrantFiled: May 30, 2003Date of Patent: March 27, 2007Assignee: Kaneka CorporationInventors: Akiko Nishi, Takumi Mano, Shinichi Yokota, Masayuki Takano, Kazuyoshi Yajima
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Publication number: 20060035363Abstract: The subject of the present invention is to provide a ?-lactam acylase protein having high activity, a gene coding for said ?-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a ?-lactam antibiotic such as amoxycillin using said ?-lactam acylase. A ?-lactam acylase gene of Stenotrophomonas maltophilia was cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and a Stenotrophomonas ?-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having ?-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.Type: ApplicationFiled: May 30, 2003Publication date: February 16, 2006Inventors: Akiko Nishi, Takumi Mano, Shinichi Yokota, Masayuki Takano, Kazuyoshi Yajima
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Publication number: 20050069996Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.Type: ApplicationFiled: December 27, 2002Publication date: March 31, 2005Inventors: Kazuyoshi Yajima, Takahisa Kato, Akihisa Kanda, Shiro Kitamura, Yasuyoshi Ueda
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Publication number: 20040234975Abstract: The present invention provides a process for producing coenzymes Q10 efficiently in microorganisms by utilizing a coenzymes Q10 side chain synthesis gene from a fungal species belonging to the genus Bulleromyces. The present invention relates to a DNA comprising a base sequence shown under SEQ ID NO:1, a DNA having a base sequence derived from the base sequence shown under SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity, or a DNA capable of hybridizing with a DNA comprising the base sequence shown under SEQ ID NO:1 under a stringent condition and encoding a protein having decaprenyl diphosphate synthase activity.Type: ApplicationFiled: April 6, 2004Publication date: November 25, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima