Patents by Inventor Kazuyoshi Yajima
Kazuyoshi Yajima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20040157286Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium.Type: ApplicationFiled: January 23, 2004Publication date: August 12, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka
-
Publication number: 20040137567Abstract: The present invention provides a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q9 or coenzyme Q10) using a microorganism.Type: ApplicationFiled: March 19, 2004Publication date: July 15, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima
-
Patent number: 6762037Abstract: The present invention has for its object to isolate a gene coding for the enzyme synthesizing the coenzyme Q10 side chain from a fungal strain of the genus Saitoella and exploit it to advantage for the efficient microbial production of coenzyme Q10. The present invention provides; a DNA having the nucleotide sequence shown under SEQ ID NO:1 a DNA having a nucleotide sequence derived from the nucleotide sequence of SEQ ID NO:1 by the deletion, addition, insertion and/or substitution of one or a plurality of nucleotides and coding for a protein having decaprenyl diphosphate synthase activity a DNA which hybridizes with the DNA having the nucleotide sequence of SEQ ID NO:1 under a stringent condition and codes for a protein having decaprenyl diphosphate synthase activity.Type: GrantFiled: July 23, 2001Date of Patent: July 13, 2004Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka, Junzo Hasegawa, Satomi Takahashi
-
Publication number: 20040067566Abstract: The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Rhodotorula.Type: ApplicationFiled: July 30, 2003Publication date: April 8, 2004Inventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka
-
Patent number: 6709378Abstract: Primaiy supernatant liquid collector 1 allows blood A to separate into a primary supernatant liquid B and red blood cells by leaving the blood A stationary for a prescribed period of time, and then it discharges the primary supernatant liquid B and physiological saline W in the same amount into a first treatment container 13 and a first balance container 14, respectively. Centrifuge 61 centrifuges the above primary supernatant liquid B using the first balance container 14 as a balance weight, and a secondary supernatant liquid in the first treatment container 13 is transferred to a second treatment container 109 by take-out device 91 and at the same time the physiological saline W in the same amount as the transferred secondary supernatant liquid is transferred from the first balance container 14 to a second balance container 110.Type: GrantFiled: April 1, 2002Date of Patent: March 23, 2004Assignees: Fujisawa Pharmaceutical Co., Ltd., Shibuya Kogyo Co., Ltd.Inventors: Shintaro Nishimura, Kazuyoshi Yajima, Satoshi Minoshima, Ichiro Matsunari, Hiroshi Myogan
-
Publication number: 20020175114Abstract: Primary supernatant liquid collecting means 1 allows blood A to separate into a primary supernatant liquid B and red blood cells by leaving the blood A stationary for a prescribed period of time, and then it discharges the primary supernatant liquid B and physiological saline W in the same amount into a first treatment container 13 and a first balance container 14, respectively.Type: ApplicationFiled: April 1, 2002Publication date: November 28, 2002Inventors: Shintaro Nishimura, Kazuyoshi Yajima, Satoshi Minoshima, Ichiro Matsunari, Hiroshi Myogan
-
Patent number: 6461842Abstract: A gene of decaprenyl diphosphate synthase, which is the key gene participating in the biosynthesis of coenzyme Q10 was isolated from a bacterium belonging to the family Rhizobiaceae. By transferring this gene into a microorganism such as Escherichia coli and expressing therein, coenzyme Q10 can be effectively produced.Type: GrantFiled: October 10, 2000Date of Patent: October 8, 2002Assignee: Kaneka CorporationInventors: Hideyuki Matsuda, Makoto Kawamukai, Kazuyoshi Yajima, Yasuhiro Ikenaka, Kenichi Nishi, Junzo Hasegawa, Satomi Takahashi
-
Patent number: 6083752Abstract: A DNA fragment coding for a decarbamylase protein improved in thermostability as the result of replacement of at least one base of a DNA fragment coding for a decarbamylase protein derived from a microorganism with another base and the resultant replacement of at least one of the corresponding amino acids, and its production process; a vector containing the DNA fragment; a transformant obtained by transformation with the vector; as well as a decarbamylase improved in thermostability and its production process. Also disclosed is a process for producing a D-.alpha.-amino acid, which comprises converting an N-carbamoyl-D-.alpha.-amino acid into the corresponding D-.alpha.-amino acid in an aqueous medium by the action of a decarbamylase having a thermostable temperature of 65.degree. C. or higher; and collecting the D-.alpha.-amino acid produced.Type: GrantFiled: June 16, 1997Date of Patent: July 4, 2000Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Yasuhiro Ikenaka, Hirokazu Nanba, Masayuki Takano, Kazuyoshi Yajima, Yukio Yamada, Satomi Takahashi
-
Patent number: 5962279Abstract: A process for the efficient production of a D-amino acid from the corresponding DL-5-substituted hydantoin by one-step reaction which comprises using a composite immobilized enzyme at a pH about neutrality, said composite immobilized enzyme being obtained by immobilizing a hydantoinase having its optimal pH within an alkaline range and a D-N-carbamyl-.alpha.-amino acid amidohydrolase having its optimal pH about neutrality in a coexisting state on an immobilizing support, simultaneously, is disclosed.Type: GrantFiled: August 16, 1996Date of Patent: October 5, 1999Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Hirokazu Nanba, Yukio Yamada, Kazuyoshi Yajima, Masayuki Takano, Yasuhiro Ikenaka, Satomi Takahashi, Takehisa Ohashi
-
Patent number: 5902736Abstract: In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.Type: GrantFiled: June 6, 1994Date of Patent: May 11, 1999Assignee: Kanegafuchi Chemical Industry Co., Ltd.Inventors: Hideaki Yamada, Sakayu Shimizu, Yasuhiro Ikenaka, Kazuyoshi Yajima, Yukio Yamada, Hirokazu Nanba, Masayuki Takano, Satomi Takahashi
-
Patent number: 5863785Abstract: In a process for the production of a D-.alpha.-amino acid, in which an N-carbamyl-D-.alpha.-amino acid corresponding to the general formula: ##STR1## wherein R represents phenyl, hydroxy-substituted phenyl, substituted or unsubstituted alkyl, or thienyl, is converted by a microbial enzyme in an aqueous medium to a D-.alpha.-amino acid corresponding to the general formula: ##STR2## wherein R is the same as defined above, decarbamylase produced by a microorganism of the genus Comamonas, Blastobacter, Alcaligenes, Sporosarcina, Rhizobium, Bradyrhizobium or Arthrobacter is used as the enzyme converting the N-carbamyl-D-.alpha.-amino acid to the D-.alpha.-amino acid.The conversion of the N-carbamyl-D-.alpha.-amino acids to the D-.alpha.-amino acids is carried out in a neutral to alkaline pH range.Type: GrantFiled: June 7, 1995Date of Patent: January 26, 1999Assignee: Kanegafuchi Chemical Industry Co., Ltd.Inventors: Hideaki Yamada, Sakayu Shimizu, Yasuhiro Ikenaka, Kazuyoshi Yajima, Yukio Yamada, Hirokazu Nanba, Masayuki Takano, Satomi Takahashi
-
Patent number: 5824522Abstract: Decarbamylases are provided capable of producing D-.alpha.-amino acids by hydrolysis of N-carbamyl-D-.alpha.-amino acids. A source of the decarbamylases is recombinant microorganisms produced by gene manipulation methods. Decarbamylases having improved thermostability can be obtained in which amino acids at a thermostability-related site of a natural decarbamylase have been replaced with other amino acids by mutating a DNA fragment encoding the natural decarbamylase. Recombinant DNA is obtained from a vector DNA and a DNA fragment encoding a natural decarbamylase where the nucleic acid sequence encoding an amino acid at a thermostability-related site is replaced with a nucleic acid sequence encoding another amino acid. The recombinant DNA is used to produce transformants that produce thermostable decarbamylases.Type: GrantFiled: August 22, 1994Date of Patent: October 20, 1998Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Yasuhiro Ikenaka, Hirokazu Nanba, Masayuki Takano, Kazuyoshi Yajima, Yukio Yamada, Satomi Takahashi, Kazuma Okubo, Kazuhiko Yamada, Yoshiro Hiraishi
-
Patent number: 5695968Abstract: The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme.The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.Type: GrantFiled: June 7, 1995Date of Patent: December 9, 1997Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Hirokazu Nanba, Yukio Yamada, Masayuki Takano, Yasuhiro Ikenaka, Satomi Takahashi, Kazuyoshi Yajima
-
Patent number: 5565344Abstract: The present invention is directed to a gene which is related to a D-N-carbamoyl-.alpha.-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-.alpha.-amino acids into D-.alpha.-amino acids; a recombinant plasmid in which a DNA fragment containing the gene is incorporated into a vector; a microorganism belonging to the genus Escherichia, Pseudomonas, Flavobacterium, Bacillus, Serratia, Corynebacterium, or Brevibacterium, which is transformed by incorporating the recombinant plasmid thereinto; a process for the production of D-N-carbamoyl-.alpha.-amino acid amidohydrolases, comprising the steps of cultivating the transformed microorganism and collecting the desired product therefrom; a D-N-carbamoyl-.alpha.-amino acid amidohydrolase obtained by the method; and a process for the production of D-.alpha.-amino acids with the aid of an action of the enzyme.The D-N-carbamoyl-.alpha.-amino acid amidohydrolase can be fixed on a support for immobilization and used as an immobilized enzyme.Type: GrantFiled: August 7, 1992Date of Patent: October 15, 1996Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Hirokazu Nanba, Yukio Yamada, Masayuki Takano, Yasuhiro Ikenaka, Satomi Takahashi, Kazuyoshi Yajima
-
Patent number: 5407819Abstract: A TPA variant with replacement of amino acids is provided. In a typical embodiment, the variant has an amino acid sequence with replacement of the amino acid group consisting of asparagine at the 37th position, serine at the 38th position, glycine at the 39th position, arginine at the 40th position, alanine at the 41st position, and glutamine at the 42nd position from the N-terminus of the amino acid sequence of mature human tissue plasminogen activator.Type: GrantFiled: April 16, 1992Date of Patent: April 18, 1995Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Hitoshi Yahara, Tetsuya Nagaoka, Kazuyoshi Yajima, Yasuhiro Inenaka, Keiji Matsumoto, Tetsu Kakutani