Abstract: Disclosed herein are methods of high-throughput mapping of viral neutralizing antibody epitopes. Also disclosed are in vitro immunoprecipitation-based adenoassociated virus Barcode-Seq-based methods of mapping viral neutralizing antibody epitopes. In some embodiments, a method of high-throughput mapping of viral NtAb conformational epitopes can be utilized, which may comprise HP scanning of mutant viral libraries, immunoprecipitation (IP), and/or next-generation sequencing (NGS) technology. In some embodiments, a method of identifying one or more dominant epitopes in a viral vector may comprise contacting a mutant capsid of a virus with serum from a subject previously exposed to the virus and immunopredpitating serum immunoglobulins from the serum. In various embodiments, the viral vector may be an AAV vector.

Abstract: An energy storage device includes: an electrode assembly including stacked plates, and a positive electrode current collector joined to the stacked plates, wherein the electrode assembly and the positive electrode current collector include a joined portion where the electrode assembly and the positive electrode current collector are joined to each other with a concavo-convex structure where either one of the electrode assembly and the positive electrode current collector projects toward the other of the electrode assembly and the positive electrode current collector, and an outer periphery of the joined portion has a non-circular shape as viewed in a stacking direction of the plates in the joined portion.

Abstract: A light emitting device includes: a base member; a laser element disposed on or above a mounting surface of the base member; a fluorescent member including a first main surface and a second main surface respectively positioned on opposite sides of the fluorescent member, the second main surface being fixed to the mounting surface of the base member; a first optical member configured to change a traveling direction of laser light emitted by the laser element to be directed toward the first main surface of the fluorescent member; and a lid connected to the base member and enclosing the laser element, the fluorescent member, and the first optical member in a space beneath the lid, the lid being configured to transmit light from the fluorescent member.

Abstract: Disclosed are adeno-associated viral vectors and plasmids encoding the same. Also disclosed are methods of using adeno-associated viral vectors to deliver a protein of interest to the subject. The disclosed vectors have phenotypes including but not limited to increased retention in the blood of a subject, avoidance of the liver, and transduction of the brain and other tissues.

Abstract: An energy storage device includes: an electrode assembly including stacked plates, and a positive electrode current collector joined to the stacked plates, wherein the electrode assembly and the positive electrode current collector include a joined portion where the electrode assembly and the positive electrode current collector are joined to each other with a concavo-convex structure where either one of the electrode assembly and the positive electrode current collector projects toward the other of the electrode assembly and the positive electrode current collector, and an outer periphery of the joined portion has a non-circular shape as viewed in a stacking direction of the plates in the joined portion.

Abstract: A light emitting device includes: a base member; a laser element disposed on or above a mounting surface of the base member; a fluorescent member including a first main surface and a second main surface respectively positioned on opposite sides of the fluorescent member, the second main surface being fixed to the mounting surface of the base member; a first optical member configured to change a traveling direction of laser light emitted by the laser element to be directed toward the first main surface of the fluorescent member; and a lid connected to the base member and enclosing the laser element, the fluorescent member, and the first optical member in a space beneath the lid, the lid being configured to transmit light from the fluorescent member.

Abstract: Disclosed herein are adeno associated viral plasmids and viral vectors. Also disclosed are methods of using adeno associated viral vectors.

Abstract: Provided is a light source device where the number of optical components is reduced without lowering an efficiency for light utilization. The light source device includes an excitation light source which generates blue laser light as excitation light, a phosphor wheel including a phosphor which is excited by the excitation light from the excitation light source to generate yellow fluorescent light, and a mirror which guides the excitation light from the excitation light source to the phosphor wheel and transmits the fluorescent light from the phosphor wheel, wherein the mirror includes a first region which reflects the excitation light and transmits the fluorescent light and a second region which transmits the fluorescent light and diffused excitation light which is diffused and reflected in the phosphor. The yellow fluorescent light and the diffused excitation light passing through the mirror are mixed to generate white light.

Abstract: A vehicle lamp includes a phosphor which receives laser light (excitation light) emitted from a semiconductor light-emitting element to emit visible light, a reflector which reflects the visible light (illumination light) emitted from the phosphor forward, and an absorbing member which absorbs excitation light regularly reflected by a surface of the phosphor. A metal plate on which the phosphor is disposed to be inclined to cause the excitation light regularly reflected by the surface of the phosphor to be incident on the absorbing member. An upper end portion of the reflector is disposed at a position lower than a vertical position of the phosphor to prevent the regularly reflected excitation light from being incident on the upper end portion.

Abstract: Disclosed herein are methods of high-throughput mapping of viral neutralizing antibody epitopes. Also disclosed are in vitro immunoprecipitation-based adenoassociated virus Barcode-Seq-based methods of mapping viral neutralizing antibody epitopes. In some embodiments, a method of high-throughput mapping of viral NtAb conformational epitopes can be utilized, which may comprise HP scanning of mutant viral libraries, immunoprecipitation (IP), and/or next-generation sequencing (NGS) technology. In some embodiments, a method of identifying one or more dominant epitopes in a viral vector may comprise contacting a mutant capsid of a virus with serum from a subject previously exposed to the virus and immunopredpitating serum immunoglobulins from the serum. In various embodiments, the viral vector may be an AAV vector.

Abstract: A light source device includes an excitation light source that generates excitation light, a phosphor wheel including a phosphor segment that generates fluorescent light by excitation of the excitation light, and a mirror that guides the excitation light from the excitation light source to the phosphor wheel and emits the fluorescent light from the phosphor wheel as illumination light. The phosphor wheel further includes an anisotropic diffusion and reflection unit that diffuses and reflects incident excitation light such that an optical path for the incident excitation light and an optical path for the diffused excitation light after incidence of the excitation light are not overlapped. The mirror includes a first region that reflects the excitation light and transmits the fluorescent light and a second region that transmits the fluorescent light and the diffused excitation light which the anisotropic diffusion and reflection unit has diffused and reflected.

Abstract: A projection image display apparatus capable of maintaining the positional accuracy of an integrator optical system even when subjected to an external force is provided.

Abstract: Provided is a vehicle lamp for emitting illumination light having reduced color nonuniformity and luminance variation with a simple structure. The vehicle lamp comprises: a light source for emitting excitation light; a fluorescent body for emitting fluorescent light excited by the excitation light emitted from the light source; a diffuser for diffusing the excitation light specularly reflected on the surface of the fluorescent body; and a reflector for reflecting the fluorescent light emitted from the fluorescent body and the excitation light diffused by the diffuser ahead of a vehicle. The fluorescent body is arranged above the light source, and the reflection surface of the reflector is arranged to face the fluorescent body from the direction in which the light is specularly reflected on the fluorescent body toward the lower side of the fluorescent body.

Abstract: Provided is a light source device where the number of optical components is reduced without lowering an efficiency for light utilization. The light source device includes an excitation light source which generates blue laser light as excitation light, a phosphor wheel including a phosphor which is excited by the excitation light from the excitation light source to generate yellow fluorescent light, and a mirror which guides the excitation light from the excitation light source to the phosphor wheel and transmits the fluorescent light from the phosphor wheel, wherein the mirror includes a first region which reflects the excitation light and transmits the fluorescent light and a second region which transmits the fluorescent light and diffused excitation light which is diffused and reflected in the phosphor. The yellow fluorescent light and the diffused excitation light passing through the mirror are mixed to generate white light.

Abstract: A vehicle lamp includes a phosphor which receives laser light (excitation light) emitted from a semiconductor light-emitting element to emit visible light, a reflector which reflects the visible light (illumination light) emitted from the phosphor forward, and an absorbing member which absorbs excitation light regularly reflected by a surface of the phosphor. A metal plate on which the phosphor is disposed to be inclined to cause the excitation light regularly reflected by the surface of the phosphor to be incident on the absorbing member. An upper end portion of the reflector is disposed at a position lower than a vertical position of the phosphor to prevent the regularly reflected excitation light from being incident on the upper end portion.

Abstract: A light source device includes: an excitation light source which generates excitation light; a phosphor wheel having a phosphor which is excited by the excitation light to generate fluorescent light; and a mirror which guides excitation light from the excitation light source to the phosphor wheel to emit the fluorescent light from the phosphor wheel as illumination light. The phosphor wheel further includes a diffusion/reflection portion which diffuses and reflects incident excitation light, and the mirror has a first region which reflects the excitation light and transmits the fluorescent light and a second region which transmits the fluorescent light and diffused excitation light. In the mirror, the fluorescent light transmitted through the first region and the fluorescent light and the diffused excitation light transmitted through the second region are used as illumination light.

Abstract: Disclosed herein are adeno associated viral plasmids and viral vectors. Also disclosed are methods of using adeno associated viral vectors.

Abstract: A projection image display apparatus capable of maintaining the positional accuracy of an integrator optical system even when subjected to an external force is provided.

Abstract: A light source device includes an excitation light source that generates excitation light, a phosphor wheel including a phosphor segment that generates fluorescent light by excitation of the excitation light, and a mirror that guides the excitation light from the excitation light source to the phosphor wheel and emits the fluorescent light from the phosphor wheel as illumination light. The phosphor wheel further includes an anisotropic diffusion and reflection unit that diffuses and reflects incident excitation light such that an optical path for the incident excitation light and an optical path for the diffused excitation light after incidence of the excitation light are not overlapped. The mirror includes a first region that reflects the excitation light and transmits the fluorescent light and a second region that transmits the fluorescent light and the diffused excitation light which the anisotropic diffusion and reflection unit has diffused and reflected.

Abstract: A display panel includes a bonded substrate which is bonded with a seal portion having an inlet portion, in which an inlet of a fluid is formed, a sealing material which seals the inlet, metal film patterns which are provided in an end portion of the bonded substrate, and external connection terminals which respectively have rectangular base portions and are provided in parallel in a transverse direction of the base portion. Each metal film pattern has a rectangular pattern base portion. The metal film patterns are provided in parallel such that the center position of the pattern base portion in the transverse direction is aligned with the center position of the base portion in the transverse direction. The width of the pattern base portion in the transverse direction is equal to or smaller than the width of the base portion in the transverse direction at the same arrangement position.