Abstract: The living cell counting method includes: a step of measuring an amount of suspensoids contained in a cell suspension; a step of measuring capacitance of the cell suspension; and a step of calculating one or more of the number of living cells, the number of dead cells, and a survival rate of cells contained in the cell suspension, based on capacitance per unit of living cells, capacitance per unit of dead cells, the amount of the suspensoids, and the capacitance of the cell suspension. A living cell counting device includes: a turbidity sensor; a measuring instrument measuring capacitance of the cell suspension; a storage unit storing data of the capacitance per unit of living cells and the capacitance per unit of dead cells; and a calculation unit calculating one or more of the number of living cells, the number of dead cells, and a survival rate of cells.

Abstract: The aseptic sampling apparatus according to the present invention includes: a sampling channel for pulling out a culture solution in a culture tank to supply the same to an analytical unit; and a pressure-applying unit for applying a pressure to the inside of the sampling channel. The pressure-applying unit controls the flow pressure or fluid flow rate of a fluid in the sampling channel such that a pressure gradient toward the outlet-side open end nozzle of the sampling channel is not less than 1 kPa/m.

Abstract: A method is provided which noninvasively monitors cells and which can accurately determine the progression of differentiation of the cells. This method, for evaluating the differentiation state of cells during culturing for inducing undifferentiated pluripotent stem cells to differentiate into desired cells, determines the progression of induced differentiation using any of the metabolites of glycolysis or any of the metabolites of the tricarboxylic acid cycle (TCA cycle), the metabolites being selected from among two or more types of amino acids contained in the culture solution or components in the culture solution derived from metabolism of the cells.

Abstract: A method and apparatus for screening of cell lines and culture conditions includes a target value setting step that sets a target value relating to a metabolic reaction of a culture cell; a first culturing step; an analysis step; an analysis value calculation step that performs a metabolism analysis based on an analysis result from the analysis step and calculates an analysis value; a culture condition computation step that computes, using a correlation model, a culture condition giving an analysis value most close to the target value, and modifies the culture condition set in the first culturing step to the computed culture condition; a second culturing step that cultures a cell under the modified culture condition; and a screening step.

Abstract: An object of the present invention is to provide an adsorbent material having high dispersibility and reversibility. The adsorbent material has a polymer material having a plurality of functional groups ionizable in water and exhibiting no lower limit critical solution temperature, an adsorption site capable of interacting with a target substance, and a carrier.

Abstract: The present invention pertains to a means and method for, from a mixture (cell culture) of cells, a medium component, and a useful substance, recovering the first two using a single process. Specifically, the present invention pertains to a polypeptide modified with a stimulus-responsive polymer, characterized in that the stimulus-responsive polymer responds to a stimulus to induce a change in the physical properties of the modified polypeptide overall, and the change is reversible or pseudo-reversible.

Abstract: A cell culture apparatus (1) of the present invention comprises: a culture vessel (2) in which a cell culture medium comprising at least one of culture components composed of a conjugate with a stimuli-responsive polymer is stored and cells are cultured in the medium; a stimulus-applying mechanism (4) that applies a predetermined stimulus to the conjugate so as to induce a predetermined change of the stimuli-responsive polymer in response to the stimulus; and, a separation mechanism (5) that separates at least a part of the medium components except for the conjugate from the cell culture medium, while leaving the conjugate in the cell culture medium, on the basis of a property change of the stimuli-responsive polymer.

Abstract: An object of the present invention is to provide an adsorbent material having high dispersibility and reversibility. The adsorbent material has a polymer material having a plurality of functional groups ionizable in water and exhibiting no lower limit critical solution temperature, an adsorption site capable of interacting with a target substance, and a carrier.

Abstract: In order to improve variety of medicines producible, a central control device that controls a plant control device for controlling a cell culture environment of a cell strain for producing a medicine is installed in a pharmaceutical manufacturing plant for manufacturing a medicine. The central control device controls the plant control device on the basis of analysis data relevant to an inspection result of a patient. The central control device transmits traffic information to a navigation device, and the navigation device searches for a delivery route of a delivery vehicle for delivering a medicine on the basis of the transmitted traffic information.

Abstract: The present invention has a subject of desorbing a target substance under mild elusion conditions in affinity purification using a low molecular weight ligand. In the present invention, a polar auxiliary group is introduced to a site adjacent to the low molecular weight ligand to perturb a pKa value of a functional group in the low-molecular-weight ligand, thereby ionizing the low molecular weight ligand under mild pH conditions.

Abstract: An object of the present invention is to separate a material to be separated at a low cost and constant accuracy when the material to be separated is separated from a mobile phase containing the material to be separated through the passing of the mobile phase through a stationary phase, even if the mobile phase has a large volume. A separation device characterized in that a separation column provided with a stationary phase having a volume capable of processing the entire volume of a mobile phase containing a material to be separated is provided, the separation column is replaceable, and the usage count of the stationary phase reaches a lifetime count through the processing of one batch.

Abstract: This invention provides a culture control method and a cell culture apparatus that enable stable cell culture by regulating shear stress to be applied to cells within an adequate range. With the application of such culture control method and cell culture apparatus, cell culture is performed under agitation culture conditions in which the shear stress distribution is 0.5 Pa to 20 Pa in 80% or more of the culture vessel by volume.

Abstract: Provided is a purification device for purifying a useful substance (i.e., target substance) without using an acidic eluent. The purification device purifying the target substance comprises a column and a filler packed in the column. The filler contains a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance.

Abstract: In order to improve variety of medicines producible, a central control device that controls a plant control device for controlling a cell culture environment of a cell strain for producing a medicine is installed in a pharmaceutical manufacturing plant for manufacturing a medicine. The central control device controls the plant control device on the basis of analysis data relevant to an inspection result of a patient. The central control device transmits traffic information to a navigation device, and the navigation device searches for a delivery route of a delivery vehicle for delivering a medicine on the basis of the transmitted traffic information.

Abstract: A transmission includes an input shaft to receive a rotation of an engine, drive gears respectively attached to the input shaft to freely rotate individually, an output shaft disposed parallel to the input shaft in order to output rotation following a gear shift, driven gears respectively attached to the output shaft to freely rotate individually and to mesh with the drive gears, an engagement mechanism to fix a gear to be used in a gear shift individually, from among the drive gears, to the input shaft to be incapable of relative rotation, and a selector mechanism to fix, during the gear shift, the driven gear of a corresponding gear position individually, which meshes with the drive gear fixed to the input shaft by the engagement mechanism, to the output shaft to be incapable of relative rotation, and a hub that is fixed to the input shaft.

Abstract: Provided are a cell separation and collection membrane capable of suppressing non-specific adsorption of cells while specifically adsorbing target cells; a culturing sheet and a culturing apparatus which use the membrane; and a cell separation and collection method. The cell separation and collection membrane included in the culturing sheet comprises: a support; a polymer site fixed to the support and having a changeable structure in accordance with a temperature; a cell adsorbing site bound to the polymer site, exposed relative to an outer surface of the support, and specifically adsorbing a target cell when the cell adsorbing site is brought into contact with a treatment solution containing the target cells; and a hydrophilic site exposed relative to the outer surface and to be brought into contact with the treatment solution.

Abstract: Provided is a purification device for purifying a useful substance (i.e., target substance) without using an acidic eluent. The purification device purifying the target substance comprises a column and a filler packed in the column. The filler contains a carrier, a temperature responsive polyamino acid bonded to the carrier, and a binding substance bonded to the temperature responsive polyamino acid and specifically bonding to the target substance.

Abstract: An object of the present invention is to separate a material to be separated at a low cost and constant accuracy when the material to be separated is separated from a mobile phase containing the material to be separated through the passing of the mobile phase through a stationary phase, even if the mobile phase has a large volume. A separation device characterized in that a separation column provided with a stationary phase having a volume capable of processing the entire volume of a mobile phase containing a material to be separated is provided, the separation column is replaceable, and the usage count of the stationary phase reaches a lifetime count through the processing of one batch.

Abstract: An adsorbent of the present invention includes a support; and a protein having an adsorption site for adsorbing a target substance, in which the protein includes a reversible binding site and a covalent binding site, the support includes an orientation-controlling site forming a reversible bond with the reversible binding site, and an immobilization site forming a covalent bond with the covalent binding site, the covalent binding site includes a nucleophilic functional group, and the immobilization site includes a functional group capable of reacting with the nucleophilic functional group by a nucleophilic substitution or a nucleophilic addition. Thus, it is possible to provide an adsorbent in which the utilization efficiency of the adsorption site of the protein is improved. As a result, the protein amount can be reduced, and the step for storing a culture solution can be simplified due to the faster purification step.

Abstract: A stimulation-responsive material comprises regions of pH-responsive polymer and regions of temperature-responsive polymer, the regions existing at different locations.