Patents by Inventor Keith S. Lowe
Keith S. Lowe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230265448Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: ApplicationFiled: October 19, 2022Publication date: August 24, 2023Applicants: E. I. DU PONT DE NEMOUS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE, KEVIN E MCBRIDE, CHRISTOPHER J SCELONGE, NING WANG
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Publication number: 20230203516Abstract: Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.Type: ApplicationFiled: August 8, 2022Publication date: June 29, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: TIM FOX, WILLIAM JAMES GORDON-KAMM, RACHEL CAROL HUEGEL, KEITH S. LOWE, JON AARON TUCKER REINDERS, HUAXUN YE
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Publication number: 20230124856Abstract: Compositions and methods for genome editing in sunflower are provided. Exemplary compositions include constructs for Cas endonuclease mediated genome editing of the FAD2-1 locus as well as sunflower plants, seeds and cells thereof which are modified using the disclosed compositions.Type: ApplicationFiled: March 23, 2021Publication date: April 20, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: GEORGE J. HOERSTER, SANDEEP KUMAR, BRIAN L LENDERTS, KEITH S LOWE
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Publication number: 20230091338Abstract: Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.Type: ApplicationFiled: February 22, 2021Publication date: March 23, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, PIERLUIGI BARONE, HYEON-JE CHO, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, BRIAN LENDERTS, KEITH S LOWE, SERGEI SVITASHEV, XINLI EMILY WU
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Patent number: 11512321Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: GrantFiled: February 22, 2021Date of Patent: November 29, 2022Inventors: William James Gordon-Kamm, Theodore M Klein, Keith S Lowe, Kevin E McBride, Christopher J Scelonge, Ning Wang
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Patent number: 11447786Abstract: Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.Type: GrantFiled: October 12, 2018Date of Patent: September 20, 2022Inventors: Tim Fox, William James Gordon-Kamm, Rachel Carol Huegel, Keith S Lowe, Jon Aaron Tucker Reinders, Huaxun Ye
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Publication number: 20220251587Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: ApplicationFiled: April 11, 2022Publication date: August 11, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
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Publication number: 20220154203Abstract: The methods disclosed herein provide for clonal plant production using a morphogenic gene to produce non-transgenic and transgenic plants wherein the morphogenic gene does not integrate into the genome of the plant.Type: ApplicationFiled: March 10, 2020Publication date: May 19, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, JON AARON TUCKER REINDERS, HUAXUN YE
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Patent number: 11332752Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: GrantFiled: March 11, 2019Date of Patent: May 17, 2022Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Stephen Lawrence Gasior, William James Gordon-Kamm, Keith S Lowe, David J Peterson, Sergei Svitashev, Joshua K Young
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Patent number: 11330776Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: GrantFiled: August 26, 2016Date of Patent: May 17, 2022Inventors: Ajith Anand, Maren L. Arling, Alexandre Da Silva Conceicao, William James Gordon-Kamm, Craig E Hastings, George J Hoerster, Todd J Jones, Theodore Mitchell Klein, Carlos M La Rota, Keith S Lowe, Shiv Bahadur Tiwari, Ning Wang, Xinli Emily Wu
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Publication number: 20220124998Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: January 7, 2022Publication date: April 28, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG HASTINGS, GEORGE J HOERSTER, TODD J JONES, THEODORE M KLEIN, CARLOS M LA ROTA, KEITH S LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
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Patent number: 11104911Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters from maize are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a maize PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.Type: GrantFiled: August 26, 2016Date of Patent: August 31, 2021Inventors: Alexandre Da Silva Conceicao, William James Gordon-Kamm, Theodore Mitchell Klein, Carlos M. La Rota, Keith S. Lowe
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Publication number: 20210180077Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: ApplicationFiled: February 22, 2021Publication date: June 17, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE, KEVIN E MCBRIDE, CHRISTOPHER J SCELONGE, NING WANG
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Patent number: 10968458Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: GrantFiled: August 10, 2017Date of Patent: April 6, 2021Inventors: William James Gordon-Kamm, Theodore Mitchell Klein, Keith S. Lowe, Kevin E. McBride, Christopher Jay Scelonge, Ning Wang
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Patent number: 10954524Abstract: Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.Type: GrantFiled: April 19, 2018Date of Patent: March 23, 2021Inventors: William J Gordon-Kamm, Timothy G Helentjaris, Keith S Lowe, Bo Shen, Mitchell C Tarczynski, Peizhong Zheng
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Patent number: 10954525Abstract: Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.Type: GrantFiled: September 27, 2018Date of Patent: March 23, 2021Inventors: William J Gordon-Kamm, Timothy G Helentjaris, Keith S Lowe, Bo Shen, Mitchell C Tarczynski, Peizhong Zheng
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Patent number: 10947550Abstract: Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.Type: GrantFiled: September 26, 2018Date of Patent: March 16, 2021Inventors: William J Gordon-Kamm, Timothy G Helentjaris, Keith S Lowe, Bo Shen, Mitchell C Tarczynski, Peizhong Zheng
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Publication number: 20210062203Abstract: The methods disclosed herein use a morphogenic gene or a repressor gene to provide a transgenic plant containing a single copy of a transgenic event wherein the morphogenic gene or the repressor gene does not integrate into the genome of the plant and the plant does not contain vector (plasmid) backbone.Type: ApplicationFiled: March 11, 2019Publication date: March 4, 2021Applicant: Pioneer Hi-Bred International, Inc.Inventors: Ajith ANAND, William James GORDON-KAMM, George J. HOERSTER, Keith S. LOWE, Kevin E. MCBRIDE
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Publication number: 20210010012Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.Type: ApplicationFiled: March 11, 2019Publication date: January 14, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
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Publication number: 20200270622Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include tissue-preferred promoters operably linked to morphogenic genes. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein operably linked to a morphogenic gene. DNA constructs comprising a promoter operably linked to a morphogenic gene and further comprising a heterologous nucleotide sequence of interest are also provided.Type: ApplicationFiled: September 19, 2018Publication date: August 27, 2020Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ALEXANDRE DA SILVA CONCEICAO, ALLISON M FINCH, WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE