Abstract: The present disclosure provides a method for determining the undifferentiated state of pluripotent stem cells, comprising: irradiating a test culture medium in which pluripotent stem cells are cultured with wavelength light having a wavelength in the range of 190 nm to 2,500 nm or a partial range thereof; detecting the reflected light, transmitted light or transmitted reflected light thereof to obtain absorbance spectrum data; and analyzing the absorbance at all or part of the measurement wavelengths in the absorbance spectrum data to determine the undifferentiated state of the pluripotent stem cells.

Abstract: An object of the present invention is to provide an image processing device, an image processing method, and a computer program that can reduce the toggle rate without degrading image data. The present invention provides an image processing device that processes image data comprising: a first processing unit, wherein the first processing unit is configured to receive input pixel data and output output pixel data, the input pixel data includes sequential pixel data which is pixel data having N sequential pixel data (N?3), and the output pixel data includes data in which the pixel data included in the sequential pixel data is sorted based on a similarity of the pixel data included in the sequential pixel data.

Abstract: The present invention relates to a method for evaluating the state of cell differentiation during the process of inducing differentiation of human induced pluripotent stem cells (iPS cells) into retinal pigment epithelial cells. The present invention further relates to a method for evaluating the state of cell differentiation during the process of inducing differentiation of human embryonic stem cells (ES cells) into retinal pigment epithelial cells.

Abstract: An object of the present invention is to provide an image processing device, an image processing method, and a computer program that can reduce the toggle rate without degrading image data. The present invention provides an image processing device that processes image data comprising: a first processing unit, wherein the first processing unit is configured to receive input pixel data and output output pixel data, the input pixel data includes sequential pixel data which is pixel data having N sequential pixel data (N?3), and the output pixel data includes data in which the pixel data included in the sequential pixel data is sorted based on a similarity of the pixel data included in the sequential pixel data.

Abstract: The purpose of the present invention is to provide a culture container capable of preventing a concave meniscus without using a jig. In order to achieve this purpose, the present invention provides a culture container (1A) provided with: a base (3) having a concave portion (4); and a water-repellent layer (5) formed on an outer edge region (411) of the bottom (41) of the concave portion (4) and the inner circumferential surface (42) of the concave portion (4). One of the surfaces of the water-repellent layer (5) is exposed to the space in the concave portion (4).

Abstract: There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

Abstract: The present disclosure provides a method for determining the undifferentiated state of pluripotent stem cells, comprising: irradiating a test culture medium in which pluripotent stem cells are cultured with wavelength light having a wavelength in the range of 190 nm to 2,500 nm or a partial range thereof; detecting the reflected light, transmitted light or transmitted reflected light thereof to obtain absorbance spectrum data; and analyzing the absorbance at all or part of the measurement wavelengths in the absorbance spectrum data to determine the undifferentiated state of the pluripotent stem cells.

Abstract: A cell-holding container comprises: an elastic body for holding cells including at least any one of adherent cells at least one selected from the group consisting of stem cells, progenitor cells, somatic cells and germ cells, and suspended cells at least one selected from the group consisting of blood cells, T cells and B cells; and the elastic body is formed of a rubber material containing a rubber component including an additional crosslinking silicone rubber and is able to hold the cells.

Abstract: A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

Abstract: The present invention is a method in which stem cells for which a differentiation state is unknown or cells that are differentiation-induced from the stem cells are use as test cells, and a differentiation state of the test cells is evaluated based on an abundance of a predetermined indicator substance in a culture supernatant of the test cells. The indicator substance is at least one compound selected from a group that consists of ornithine, 2-aminoadipic acid, deoxycytidine, glutamic acid, tryptophan, asparagine, alanine, cystine, hypoxanthine, uridine, aspartic acid, arginine, 2-hydroxybutyric acid, 2-hydroxyisovaleric acid, 3-hydroxyisovaleric acid, urea, 4-hydroxybenzoic acid, 4-aminobenzoic acid, ribonic acid, kynurenine, crystathionine, threonic acid, pyruvic acid, putrescine, ascorbic acid, riboflavin, serine, cysteine, orotic acid, and citric acid.

Abstract: A cell-holding container comprises: an elastic body for holding cells including at least any one of adherent cells at least one selected from the group consisting of stem cells, progenitor cells, somatic cells and germ cells, and suspended cells at least one selected from the group consisting of blood cells, T cells and B cells; and the elastic body is formed of a rubber material containing a rubber component including an additional crosslinking silicone rubber and is able to hold the cells.

Abstract: The purpose of the present invention is to provide a culture container capable of preventing a concave meniscus without using a jig. In order to achieve this purpose, the present invention provides a culture container (1A) provided with: a base (3) having a concave portion (4); and a water-repellent layer (5) formed on an outer edge region (411) of the bottom (41) of the concave portion (4) and the inner circumferential surface (42) of the concave portion (4). One of the surfaces of the water-repellent layer (5) is exposed to the space in the concave portion (4).

Abstract: A buffer tank for storing a culture medium for cell culture, includes: a tank main body including a cylindrical inner surface extending in a vertical direction, an inverted conical storage bottom surface formed below the cylindrical inner surface, and a storage space defined by the cylindrical inner surface and the inverted conical storage bottom surface and configured to store the culture medium an injection portion configured to inject the culture medium from the inverted conical storage bottom surface of the tank main body into the storage space; and a discharge portion configured to discharge the culture medium stored in the storage space, wherein the injection portion is configured to inject the culture medium toward a central axis line of the cylindrical inner surface of the tank main body and obliquely upward with respect to the central axis line.

Abstract: There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

Abstract: A cell separation device imparts vibration to a cell culture film having a front surface to which cells adhere, from a rear surface side of the film, and consequently separates the cells from the film. The device includes an ultrasonic probe having an ultrasonically-vibrating vibrator, a frame disposed to surround the vibrator and a first detection part configured to detect a force applied to the frame in a specified direction. The vibrator selectively imparts ultrasonic vibration to specified cells adhering to the front surface of the film. Based on a detection result obtained by the first detection part, the frame is pushed until the force applied from the rear surface of the film to the frame reaches a specified magnitude. Thereafter, the vibrator ultrasonically vibrates in a state in which the frame makes contact with the rear surface of the film.

Abstract: A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

Abstract: Provided is a method for producing a filtration filter capable of purifying with high accuracy and largely improving filtration efficiency. A flow path forming film is formed on a substrate. A plurality of grooves is formed on the flow path forming film along a surface of the substrate by etching. The grooves are filled with a sacrificial film. The flow path forming film and the sacrificial film are planarized by polishing the sacrificial film. A flow path sealing film is formed on the planarized flow path forming film and sacrificial film. An inlet hole and an outlet hole are formed through the substrate and the flow path sealing film, respectively, so that parts of the sacrificial film are exposed through the inlet hole and the outlet hole. The sacrificial film is removed using the inlet hole and the outlet hole and the filtration flow paths are formed by the grooves.

Abstract: In a manufacturing method for an interposer, a seed layer is formed at an opening portion in a through hole on back surface side of a substrate, an electrode layer for electroplated coating is formed based on the seed layer, and an electroplated coating layer is formed to fill the through hole from the electrode layer for electroplated coating layer to a front surface side. As a result, a manufacturing method for an interposer is provided in which the manufacturing process is simple and the void is not generated inside of the through hole.

Abstract: The present invention provides an electromagnetically actuating optical deflecting elements which can be manufactured out of reduced number of components and are capable of being actuated at lower frequencies and at wider deflecting angles without causing mechanical influences of the metal wiring on beams of the optical deflecting element. For that purpose, the electromagnetically actuating optical deflecting element comprises: a movable part having a light reflecting plane and a coil; a base component having a magnetic field generating means; and a pair of beams which axially support said movable part on to the base component, The movable part is actuated by an electromagnetic force generated by an electric current flowing through the coil and the magnetic field generating means.

Abstract: A through substrate which comprises a silicon substrate (10) having a through hole (19) penetrating a front surface (11) and a back surface (12), a oxidized silicon film (13) being provided along the inner wall surface of the through hole (19), layers (14, 15) comprising Zn and Cu, respectively, being formed on the inner wall surface of the oxidized silicon film (13), and a Cu plating layer (18) which has been grown from a Cu seed layer (17) along the inner wall surface of layers (14, 15) comprising Zn and Cu, respectively, via an insulating layer (16) between them. The above through substrate can provide a through electrode capable of avoiding the noise due to the cross talk.