Abstract: As a technology for enriching atrial myocytes or ventricular myocytes in a cardiomyocyte-containing cell population induced from stem cells, provided is a method for enriching atrial myocytes or ventricular myocytes in a cardiomyocyte-containing cell population induced from stem cells, the method including a step of collecting atrial myocytes or ventricular myocytes from the cell population by using the expression level of CD151 as an index.

Abstract: A non-crosslinked rubber laminate which is a rubber laminate includes a butyl rubber-containing layer stacked with an ethylene propylene diene rubber and/or ethylene propylene rubber-containing layer, wherein such non-crosslinked rubber laminate is characterized in that the ethylene propylene diene rubber and/or ethylene propylene rubber-containing layer is a rubber-containing layer that contains a low-molecular-weight polyolefin, a phenolic resin, and a crosslinking agent, where: the low-molecular-weight polyolefin is contained by 40 to 70 parts by weight, the phenolic resin is contained by 1 to 4 parts by weight, and the crosslinking agent is contained by at least 1 part by weight, relative to the ethylene propylene diene rubber and/or ethylene propylene rubber constituting a total of 100 parts by weight. An air conditioning hose made of the rubber laminate, for example, can accommodate POE-based lubricating oils and retain vibration absorption characteristics.

Abstract: The present invention provides a heterocyclic compound having an activity to promote the maturation of a cardiomyocyte. A compound represented by the formula (I): wherein each symbol is as defined in the description, or a salt thereof has an activity to promote the maturation of a cardiomyocyte, and is useful as a cardiomyocyte maturation promoter.

Abstract: The present invention provides means which is excellent in efficiency with which a nucleic acid is introduced to cardiac muscle cells, especially a transfection method. A method for transfection of a nucleic acid into a cardiac muscle cell of the present invention comprises a step of contacting a composition comprising: a compound represented by the formula (I) or a salt thereof; a structured lipid; and a nucleic acid, with a cell population comprising cardiac muscle cells.

Abstract: A production method comprising the step of forming an aggregate of cells under conditions that permit transfection of the cells with a substance in a non-cell-adhesive container is provided as a technique for producing cells having the desired substance introduced therein on a large scale at a commercial level.

Abstract: The present invention provides a heterocyclic compound having an activity to promote the maturation of a cardiomyocyte. A compound represented by the formula (I): wherein each symbol is as defined in the description, or a salt thereof has an activity to promote the maturation of a cardiomyocyte, and is useful as a cardiomyocyte maturation promoter.

Abstract: The present invention provides a cardiomyocyte maturation promoter. The present invention provides a cardiomyocyte maturation promoter comprising one or more compounds selected from 2-methoxy-5-((Z)-2-(3,4,5-trimethoxyphenyl)vinyl)phenol, (1-ethyl-1H-benzotriazol-5-yl)methyl (2-(2-methoxy-4-methylphenyl)-4-methyl-1,3-thiazol-5-yl)carbamate, (2?beta)-22-oxovincaleukoblastine, 2-(2-(4-chlorophenyl)ethyl)-6-(2-furyl)-3H-imidazo[4,5-b]pyridine, 4,5-anhydro-1,2-dideoxy-4-methyl-2-((N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl)amino)-1-phenyl-L-threo-pent-3-ulose, 3-(3-methoxyphenyl)-N7,N7-dimethylisoquinoline-1,7-diamine, methyl 4-(2-benzylbenzoyl)-2,5-dimethyl-1H-pyrrole-3-carboxylate, 2?-(4-aminophenyl)-1H,1?H-2,5?-bibenzimidazol-5-amine, and salts thereof.

Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.

Abstract: A non-crosslinked rubber laminate which is a rubber laminate includes a butyl rubber-containing layer stacked with an ethylene propylene diene rubber and/or ethylene propylene rubber-containing layer, wherein such non-crosslinked rubber laminate is characterized in that the ethylene propylene diene rubber and/or ethylene propylene rubber-containing layer is a rubber-containing layer that contains a low-molecular-weight polyolefin, a phenolic resin, and a crosslinking agent, where: the low-molecular-weight polyolefin is contained by 40 to 70 parts by weight, the phenolic resin is contained by 1 to 4 parts by weight, and the crosslinking agent is contained by at least 1 part by weight, relative to the ethylene propylene diene rubber and/or ethylene propylene rubber constituting a total of 100 parts by weight. An air conditioning hose made of the rubber laminate, for example, can accommodate POE-based lubricating oils and retain vibration absorption characteristics.

Abstract: An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.

Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.

Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.

Abstract: An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.

Abstract: The present invention provides a method for efficiently producing cardiomyocytes from pluripotent stem cells, which method comprises the steps of dissociating embryoid bodies obtained during the production process, and allowing reaggregation of the resulting cells to allow formation of embryoid bodies.

Abstract: A game apparatus includes a CPU, and the CPU executes game processing of a golf game, and displays a guide path of a circle a part of which is erased in a hitting operation. When the player performs a sliding operation in a clockwise direction so as to trace the guide path, and then inverts the sliding operation in a counterclockwise direction, the player character performs a swing motion according thereto. As to each of backswing, downswing and follow-through, precision, a speed, smoothness of the sliding operation are determined, and in accordance with the results, the movement of a ball is controlled.

Abstract: The present invention provides a method for efficiently producing cardiomyocytes from pluripotent stem cells, which method comprises the steps of dissociating embryoid bodies obtained during the production process, and allowing reaggregation of the resulting cells to allow formation of embryoid bodies.

Abstract: The invention provides a connector including a body having insulating properties, a conductive shield case surrounding the body, and a first terminal group arrayed in a line in the body. The first terminal group includes a first terminal and a second terminal. The second terminal is disposed adjacent to the first terminal and having a higher impedance than the first terminal. The shield case includes an adjacent portion that is adjacent to at least a portion of the second terminal and on an opposite side to the first terminal. At least one of the portion of the second terminal and the adjacent portion of the shield case is extended in width so as to shorten a distance between the portion of the second terminal and the adjacent portion of the shield case in accordance with an impedance difference between the first terminal and the second terminal.

Abstract: A game apparatus includes a CPU, and the CPU executes game processing of a golf game, and displays a guide path of a circle a part of which is erased in a hitting operation. When the player performs a sliding operation in a clockwise direction so as to trace the guide path, and then inverts the sliding operation in a counterclockwise direction, the player character performs a swing motion according thereto. As to each of backswing, downswing and follow-through, precision, a speed, smoothness of the sliding operation are determined, and in accordance with the results, the movement of a ball is controlled.

Abstract: The invention provides a connector including a body having insulating properties, a conductive shield case surrounding the body, and a first terminal group arrayed in a line in the body. The first terminal group includes a first terminal and a second terminal. The second terminal is disposed adjacent to the first terminal and having a higher impedance than the first terminal. The shield case includes an adjacent portion that is adjacent to at least a portion of the second terminal and on an opposite side to the first terminal. At least one of the portion of the second terminal and the adjacent portion of the shield case is extended in width so as to shorten a distance between the portion of the second terminal and the adjacent portion of the shield case in accordance with an impedance difference between the first terminal and the second terminal.

Abstract: The invention provides a connector in which, in an over-mold process, it is possible to prevent a molten resin from flowing through inevitable gaps (88, 89) of a shell (80) into the inside of the shell, thereby preventing problems such as that movable portions of contacts (20) become immovable, from arising. The connector of the invention has a plug body (10) in which contacts (20) to be connected to a cable (70) are held by an insulative body (40), the body (40) is shielded by a shell (80) formed by a metal plate, and a part of the plug body (10) is over-molded. The body (40) has sealing portions (41, 42) which are fitted into gaps (88, 89) of the shell (80) to close the gaps (88, 89).