Patents by Inventor Kenneth Michael Evans
Kenneth Michael Evans has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9222872Abstract: A nozzle tip formed from a cylindrical body defining a longitudinal axis and a frustoconical body adjoining the cylindrical body on the longitudinal axis. The cylindrical body may be in fluid communication with the frustoconical body. The frustoconical body may end in a flat surface with a nozzle exit orifice which is transverse to the longitudinal axis. There may be a cutout at the edge of the frustoconical body and the flat surface. The flow cytometer system may also include a source of electromagnetic radiation for producing a beam incident upon the fluid stream and the particles and a detector for detecting light emitted or reflected from the particles within the fluid stream in response to the beam.Type: GrantFiled: September 19, 2013Date of Patent: December 29, 2015Assignee: Inguran, LLCInventors: Kris Buchanan, Kenneth Michael Evans
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Patent number: 9140688Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: GrantFiled: September 28, 2012Date of Patent: September 22, 2015Assignee: Inguran, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20150219546Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.Type: ApplicationFiled: March 4, 2013Publication date: August 6, 2015Applicant: Inguran, LLCInventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
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Patent number: 9027850Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.Type: GrantFiled: March 14, 2013Date of Patent: May 12, 2015Assignee: Inguran, LLCInventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
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Publication number: 20150072340Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: ApplicationFiled: September 28, 2012Publication date: March 12, 2015Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Publication number: 20140234833Abstract: A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.Type: ApplicationFiled: September 28, 2012Publication date: August 21, 2014Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas B. Gilligan, Clara Gonzalez-Marin
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Patent number: 8703418Abstract: Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).Type: GrantFiled: January 28, 2013Date of Patent: April 22, 2014Assignee: XY, LLCInventors: Kenneth Michael Evans, Erik B. van Munster
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Publication number: 20140099664Abstract: This disclosure relates to cell sorting methods, and particularly cell sorting methods that improve the efficiency or productivity of sorting in a particle sorting instrument utilizing a measured parameter of sorting efficiency. In one embodiment, minimum productivity and minimum purity may be established and maintained while attempting to maximize the sorting efficiency. While in another embodiment, a minimum sorting efficiency and a minimum purity may be established and maintained while attempting to maximize the productivity of a sort.Type: ApplicationFiled: March 4, 2013Publication date: April 10, 2014Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Johnathan Charles Sharpe, Juan Moreno, Ramakrishnan Vishwanath
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Publication number: 20140099628Abstract: Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.Type: ApplicationFiled: October 3, 2013Publication date: April 10, 2014Applicant: INGURAN, LLCInventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Richard W. Lenz, Ramakrishnan Vishwanath
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Publication number: 20140099627Abstract: Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.Type: ApplicationFiled: March 4, 2013Publication date: April 10, 2014Applicant: INGURAN, LLCInventors: Thomas Boyd Gilligan, Kenneth Michael Evans, Richard Lenz, Clara Gonzalez-Marin, Ramakrishnan Vishwanath
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Publication number: 20140076986Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.Type: ApplicationFiled: March 14, 2013Publication date: March 20, 2014Applicant: INGURAN, LLCInventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
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Publication number: 20140076994Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.Type: ApplicationFiled: March 14, 2013Publication date: March 20, 2014Applicant: INGURAN, LLCInventors: Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
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Publication number: 20140078502Abstract: A nozzle tip formed from a cylindrical body defining a longitudinal axis and a frustoconical body adjoining the cylindrical body on the longitudinal axis. The cylindrical body may be in fluid communication with the frustoconical body. The frustoconical body may end in a flat surface with a nozzle exit orifice which is transverse to the longitudinal axis. There may be a cutout at the edge of the frustoconical body and the flat surface. The flow cytometer system may also include a source of electromagnetic radiation for producing a beam incident upon the fluid stream and the particles and a detector for detecting light emitted or reflected from the particles within the fluid stream in response to the beam.Type: ApplicationFiled: September 19, 2013Publication date: March 20, 2014Applicant: Inguran, LLCInventors: Kris Buchanan, Kenneth Michael Evans
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Patent number: 8603396Abstract: Particular embodiments of the inventive technology relate to ‘off-axis detector’ technology that employs a third detector 23 exhibiting a flow orthogonal axis 43 as defined by its EMR collection angle, where such axis is from 30 degrees to 60 degrees from an intended, flow orthogonal, cell cross section long axis alignment line 13, in addition to employing a fourth detector 24 exhibiting a flow orthogonal axis 44 as defined by its collection angle, where such axis is orthogonal to the flow orthogonal, third detector axis 43. Particular embodiments of the inventive technology relate to ‘axially spaced illumination’ technology featuring more than one cell illumination site, where, other than the most upflow illumination site (e.g., 121), all illumination site(s) are downflow of at least one other illumination site. Particular embodiments of the inventive technology may feature aspects of both technologies.Type: GrantFiled: August 21, 2008Date of Patent: December 10, 2013Assignee: XY, LLCInventors: Johnathan Sharpe, Kenneth Michael Evans
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Publication number: 20130011825Abstract: A method and system for processing reproductive cell samples and for sorting sperm with reduced levels and occurrences of DNA fragmentation compared to conventional sorting and processing methods, and for using reproductive cell and sperm cell samples with low levels of DNA fragmentation to improve viability of insemination samples fertility and success rates of assisted reproductive procedures, including artificial insemination, in vitro fertilization, intracytoplasmic injection, and other related techniques.Type: ApplicationFiled: December 30, 2010Publication date: January 10, 2013Applicant: INGURAN, LLCInventors: Juan F. Moreno, Kenneth Michael Evans, Michael Kjelland, Jamie Gosalvez, Clara Gonzalez-Martin, Carmen Lopez-Fernandez
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Publication number: 20120301869Abstract: A device, system and method for the separation and collection of sperm cells into multiple subpopulations based upon sperm characteristics and for facilitating the collection of multiple subpopulations in limited space. The device can be a redirection device including one or more spaced apart tubes, where each tube has a tube inlet for collecting particles in a flow path, a tube outlet for dispensing the collected particles, and a tube body connecting the tube inlet to the tube outlet for redirecting the particles in the flow path. The device can further include a support for holding each tube in a spaced apart relationship and a securing element for securing the one or more spaced apart tubes to the support.Type: ApplicationFiled: May 25, 2012Publication date: November 29, 2012Applicant: INGURAN, LLCInventor: Kenneth Michael Evans
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Publication number: 20110143389Abstract: Particular embodiments of the inventive technology relate to ‘off-axis detector’ technology that employs a third detector 23 exhibiting a flow orthogonal axis 43 as defined by its EMR collection angle, where such axis is from 30 degrees to 60 degrees from an intended, flow orthogonal, cell cross section long axis alignment line 13, in addition to employing a fourth detector 24 exhibiting a flow orthogonal axis 44 as defined by its collection angle, where such axis is orthogonal to the flow orthogonal, third detector axis 43. Particular embodiments of the inventive technology relate to ‘axially spaced illumination’ technology featuring more than one cell illumination site, where, other than the most upflow illumination site (e.g., 121), all illumination site(s) are downflow of at least one other illumination site. Particular embodiments of the inventive technology may feature aspects of both technologies.Type: ApplicationFiled: August 21, 2008Publication date: June 16, 2011Applicant: XY, LLCInventors: Johnathan Sharpe, Kenneth Michael Evans
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Publication number: 20110076712Abstract: A flow cytometer having a nozzle assembly which provides a lubricious flow path through which a plurality of particles pass to be entrained in a flow of liquid to provide a faster method for sorting with increased cell quality.Type: ApplicationFiled: June 12, 2009Publication date: March 31, 2011Applicant: XY, LLC.Inventors: Thomas Boyd Gilligan, Kenneth Michael Evans