Abstract: A lateral flow assay and compositions for performing such an assay are described herein that detect whole virus (e.g., HIV, HCV, HSV-2) or viral proteins that use the broad-spectrum anti-viral lectin, griffisthin (GRFT), conjugated to polymeric or gold nanoparticles (NPs) and an appropriate monoclonal antibody (mAb) to confer virus selectivity.

Abstract: Described herein are antimicrobial (e.g., antibacterial, antiviral) compositions and methods of using such compositions.

Abstract: A composition is provided comprising an electrospun fiber having a surface with a biological adhesive moiety conjugated to the surface of the electrospun fiber. The biological adhesive moiety included in the composition can be a lectin such as griffithsin. The composition can further include an effective amount of an antiviral agent encapsulated by an electrospun fiber. Nanoparticles including a microbicide conjugated to the surface of the nanoparticle can also be included in the composition. Methods of treating a viral infection are also provided and include administering to a subject an effective amount of a composition comprising an electrospun fiber having a surface and a biological adhesive moiety conjugated to the surface of the electrospun fiber.

Abstract: A composition is provided comprising an electrospun fiber having a surface with a biological adhesive moiety conjugated to the surface of the electrospun fiber. The biological adhesive moiety included in the composition can be a lectin such as griffithsin. The composition can further include an effective amount of an antiviral agent encapsulated by an electrospun fiber. Nanoparticles including a microbicide conjugated to the surface of the nanoparticle can also be included in the composition. Methods of treating a viral infection are also provided and include administering to a subject an effective amount of a composition comprising an electrospun fiber having a surface and a biological adhesive moiety conjugated to the surface of the electrospun fiber.

Abstract: A card game includes a deck of playing cards, an octahedral die, and spinner with a category card and timer. Each face of the octahedral die has indicia designating the dealer for the game. The spinner is divided into sections. A rotatable pointer is attached to the spinner. A category card is associated with the spinner and has verbiage describing the variants of the card game selected by the pointer. A timer controls the time of a speed hand and is started at the beginning of play of the card game. Prior to beginning of play of the card game, the octahedral die is thrown and when it had landed, the side facing up decides the dealer. Another player then spins the rotatable pointer to decide the category variant of the game to be played. Play then proceeds as with the rules of the card game.

Abstract: A composition is provided comprising an electrospun fiber having a surface with a biological adhesive moiety conjugated to the surface of the electrospun fiber. The biological adhesive moiety included in the composition can be a lectin such as griffithsin. The composition can further include an effective amount of an antiviral agent encapsulated by an electrospun fiber. Nanoparticles including a microbicide conjugated to the surface of the nanoparticle can also be included in the composition. Methods of treating a viral infection are also provided and include administering to a subject an effective amount of a composition comprising an electrospun fiber having a surface and a biological adhesive moiety conjugated to the surface of the electrospun fiber.

Abstract: A golf training aid comprises a body including a top surface and a bottom surface. The top surface comprises a club shaft groove. The bottom surface comprises an index finger groove, a middle finger groove and a separation bulge between the two index and middle finger grooves. The body comprises an elastomeric material having sufficient flexibility so the club shaft groove generally conforms to a golf club shaft and the index and middle finger grooves generally conform to the index and middle fingers of a user when the body is positioned between the user's hand and the golf club shaft with a user's hand gripping the golf club shaft.

Abstract: Compositions for treating papillomavirus (PV) infection in a subject include a PV L2 polypeptide produced from a eukaryotic expression system.

Abstract: A deflector (1) for attachment to a spiral separator (2), for capturing and redirecting a portion of a flowing stream of material (5). The deflector (1) includes an attachment means (3), to attach the deflector (1) to the spiral separator (2), a capturing portion (4) to capture a portion of the flowing stream (5), and, a redirecting portion (6) to emit a portion (7) of the stream. A method of separating particles of different densities using the deflector device (1) in conjunction with a spiral separator (2) is also disclosed.

Abstract: Compositions for treating papillomavirus (PV) infection in a subject include a PV L2 polypeptide produced from a eukaryotic expression system.

Abstract: A packing insert is shown, which includes a first face, a second face, opposite the first face, a first side support and a second side support. The first side support is flexibly connected to the first face and the second face, preferably to one edge of each face. When not fully extended, the side supports bend inwards between the first face and the second face, and they are in positions with respect to the first face and the second face and each other such that when they are bent inwards between the first face and the second face, the first support member does not interfere with the second support member. In different arrangement, each side support includes a forward support member flexibly connected at one end to one edge of the first face and a rear support member flexibly connected at one end to one edge of the second face. The forward support member and the rear support member are rotatably connected to each other at their ends which are opposite the first face and the second face.

Abstract: The present invention relates to plant produced native aprotinin and aprotinin variants having enzyme-inhibitory, immunological and pharmacokinetic properties and their preparation. In a preferred method a recombinant RNA plant virus is used to express native aprotinin+variants thereof in Nicotiana plants.

Abstract: Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

Abstract: The invention provides a fusion protein comprising a plant virus coat protein and a GDF8 peptide domain, or antigenic fragment of the GDF8 peptide domain. Plant virus vectors expressing the fusion protein and methods of using these vectors are also provided.

Abstract: A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins of interest. A multi-channel apparatus processes the various green juices, one green juice per channel. The apparatus is computer controlled such that the various valves in each channel and pump are controlled in an automated manner to extract each protein of interest and deliver each protein of interest into its own storage vessel.

Abstract: Juvenile-onset recurrent respiratory papillomatosis is treated using active vaccination or passive immune therapy of neutralizing antibodies against HPV L2 neutralizing epitopes.

Abstract: A rolling circle DNA replicon which replicates in a host eukaryotic cell is disclosed which has a truncated replication cycle. The rolling circle DNA replicon comprises the following elements present on the same DNA molecule. It contains a Rep gene open reading frame from a virus belonging to the viral taxonomic families Geminiviridae, Circoviridae or genus Nanovirus. The Rep gene open reading frame is placed under transcriptional control of a promoter, which is placed 5? of the gene. Any sequences that are required to be present in cis on the rolling circle DNA replicon in order that the Rep protein might promote replication of the rolling circle DNA replicon are included. An expression cassette for expression of an ancillary protein that is capable of creating a cellular environment permissive for replication of the rolling circle DNA replicon in the host cell of interest is also included.

Abstract: Herein described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into coat proteins or encapsidation intermediate populations. Each population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different coat proteins or encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different coat proteins such that the reassembled VLP displays more than one peptide or other molecule. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.