Abstract: Provided herein are reagents for improving PCR accuracy.

Abstract: Provided herein are reagents for improving PCR accuracy.

Abstract: Provided herein are reagents for improving PCR accuracy.

Abstract: Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where different target amplicons may have identical probe hybridization sequences by employing multiple temperature end-point signal probe detection. Also provided herein are methods, kits, and compositions for distinguishing between two or more target amplicons using multiple-temperature end-point probe detection. In certain embodiments, asymmetric PCR amplification methods are employed (e.g., LATE-PCR amplification).

Abstract: A non-symmetric polymerize chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.

Abstract: Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of various Staphylococcus species and types. In particular, provided herein are assays that allow the detection of MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, and MRSA community strain by detecting the presence of absence of one or more target sequences selected from a list of target genes and sequences that include, but are not limited to: mecA, femA-SA, femA-SE, lukF-PV, lukS-PV, VanA, SCCmec type I, type II, type III, type IV, type V, and type VII. In certain embodiments, asymmetric PCR amplification methods are employed. In other embodiments, probe-target generated signals are detected at least two temperatures to generate a temperature/temperature signal ratio.

Abstract: A lightweight tender comprising a hull constructed from substantially a single piece of composite material and a retractable pillar attached to the hull, wherein the retractable pillar defines a longitudinally extending channel. A retractable carbon fiber bimini cover is pivotingly attached to the retractable pillar, and defines a top deck when the pillar is retracted, and defines a bimini top extended. A first end of a brace is hingedly attached to the bimini cover and a second end of the brace travels within the longitudinally extending channel of the retractable pillar such that the angle between the bimini cover and the retractable pillar increases as the brace travels distally within the longitudinally extending channel.

Abstract: Provided herein are reagents for improving PCR accuracy.

Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.

Abstract: A lightweight tender comprising a hull constructed from substantially a single piece of composite material and a retractable pillar attached to the hull, wherein the retractable pillar defines a longitudinally extending channel. A retractable carbon fiber bimini cover is pivotingly attached to the retractable pillar, and defines a top deck when the pillar is retracted, and defines a bimini top extended. A first end of a brace is hingedly attached to the bimini cover and a second end of the brace travels within the longitudinally extending channel of the retractable pillar such that the angle between the bimini cover and the retractable pillar increases as the brace travels distally within the longitudinally extending channel.

Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.

Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.

Abstract: Provided herein are methods, kits, and compositions related to nucleic acid detection that allow the detection and discrimination of various Staphylococcus species and types. In particular, provided herein are assays that allow the detection of MSSA, MSSE, MRSA, MRSE, VRSA, VRSE, and MRSA community strain by detecting the presence of absence of one or more target sequences selected from a list of target genes and sequences that include, but are not limited to: mecA, femA-SA, femA-SE, lukF-PV, lukS-PV, VanA, SCCmec type I, type II, type III, type IV, type V, and type VII. In certain embodiments, asymmetric PCR amplification methods are employed. In other embodiments, probe-target generated signals are detected at least two temperatures to generate a temperature/temperature signal ratio.

Abstract: Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where different target amplicons may have identical probe hybridization sequences by employing multiple temperature end-point signal probe detection. Also provided herein are methods, kits, and compositions for distinguishing between two or more target amplicons using multiple-temperature end-point probe detection. In certain embodiments, asymmetric PCR amplification methods are employed (e.g., LATE-PCR amplification).

Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.

Abstract: Homogenous detection during or following PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled primers and probes, improves reproducibility and quantification. Low-temperature homogeneous detection during or following non-symmetric PCR amplification, preferably LATE-PCR, utilizing fluorescent DNA dye and indirectly excitable labeled mismatch-tolerant probes permits analysis of complex targets. Sequencing sample preparation methods following LATE-PCR amplifications reduce complexity and permit “single-tube” processing.

Abstract: An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.

Abstract: An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.

Abstract: A non-symmetric polymerise chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.

Abstract: Program execution can be monitored and recorded for later playback. Certain state changes that can be predicted via a virtual processor during playback need not be recorded, so a compressed recording can be stored. To facilitate random access with respect to time during playback, key frames can be stored within the compressed recording. An index mechanism can associate key frames with particular memory addresses. Additionally, a snapshot of values for memory addresses can be used to further facilitate determining the value of a memory address without having to simulate execution. Multiprocessor executions can be supported, and playback can be done on a machine type different from that on which recording took place.