Abstract: A communication system and a communication method arranged to obtain a diversity effect in mobile communication, to reduce the influence of multipath interference, fading or the like, to perform comparatively simple information communication such as message communication with improved reliability, and to achieve effective utilization of frequencies. A plurality of base stations having receivers are provided around an urban area within base station zones. The base stations repeatedly transmit the same message data. Receiving repeaters are provided in the urban area and small or medium suburban zones. Each receiving repeater relays a signal from a small mobile terminal via an Internet network if the output level of the mobile terminal is so low that direct transmission to one of the base stations is impossible. A 25 kHz frequency band is divided into four narrower bands of 6.25 kHz in one of the embodiments.

Abstract: A method of checking and acknowledging reception of a message in a two-way radio communication system without causing a reduction in the efficiency of processing the message, a storage medium on which a reception checking and acknowledging program is stored for use in the two-way radio communication system, and a two-way radio communication system and a base station for use in the two-way radio communication system is provided. If message data transmitted by a terminal device is received by a base station via a relay station and an Internet network, the base station generates reception acknowledgment data and transmits it in such a manner that when downstream message data is transmitted toward a plurality of terminal devices, the above-described reception acknowledgment data is added to the downstream message data and the resultant data is transmitted to the plurality of terminal devices.

Abstract: Methods and compositions are provided for regulated expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide (or an insertion site therefor) linked for transcriptional expression in reading phase with one or more functional fragments derived from an outer membrane protein gene of a Gram-negative bacterium. The plasmids also include an inducible promoter sequence positioned in the proper orientation for transcriptional expression of the desired polypeptide, as well as a separate DNA sequence coding for a repressor molecule which can interact with the inducible promoter to prevent transcription therefrom. Expression of the desired polypeptide is under the control of both the constitutive promoter and the inducible promoter, although transcription from either promoter is normally blocked by the repressor molecule.

Abstract: Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gram-negative bacterium, and also linked with an additional promoter sequence in certain cases. The methods utilize such plasmids to introduce genetic capability into microorganisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.

Abstract: Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gramnegative bacterium. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.