Abstract: This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types.

Abstract: A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

Abstract: Bifunctional molecules having first and second alpha-synuclein binding agents coupled by a linker are disclosed. The bifunctional molecules have potential utility in the diagnosis, treatment and/or prophylaxis of disorders in which alpha-synuclein is implicated, including Parkinson's disease. Methods of making and using the bifunctional molecules are disclosed.

Abstract: Disclosed are devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. The devices include a non-woven fabric external to a cell-excluding membrane, and the non-woven fabric and/or cell-excluding membrane can be perforated. Treatment of the host with immunosuppressive reagents, required to inhibit allograft rejection due to perforations in the cell delivery device, does not compromise maturation or function of transplanted pancreatic endoderm cells.

Abstract: Disclosed herein are cell cultures comprising dorsal and/or ventral PDX1-positive foregut endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified dorsal and/or ventral PDX1-positive foregut endoderm cells as well as methods for enriching, isolating and purifying dorsal and/or ventral PDX1-positive foregut endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of dorsal and/or ventral PDX1-positive foregut endoderm cells, are also disclosed.

Abstract: A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

Abstract: Disclosed herein are cell cultures comprising dorsal and/or ventral PDX1-positive foregut endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified dorsal and/or ventral PDX1-positive foregut endoderm cells as well as methods for enriching, isolating and purifying dorsal and/or ventral PDX1-positive foregut endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of dorsal and/or ventral PDX1-positive foregut endoderm cells, are also disclosed.

Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise reverse transcribing mRNA to produce DNA:mRNA hybrids, treating the DNA:mRNA hybrids with RNAseH to produce mRNA fragments, and reverse transcribing the mRNA fragments.

Abstract: In one embodiment, a system and method of predicting sale transaction conversion rate of an item operates through a search of information in response to a query over a network. The item can be included in a category of items. Information for other relevant items of the category is available through network query and historical data, among others. Respective information for the other items of the category is available to the method.

Abstract: Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.

Abstract: The present invention is an apparatus for tire inflation designed to allow users to properly inflate, maintain, and monitor the condition of vehicle tires. The tire inflation device of present invention is comprising of: a carrying case having a housing, a lid attached to the housing using a hinge, a plurality of locking latches to lock the lid over the housing and a handle configured over the lid to allow easy transport of the tire inflating device. The tire inflation device further comprises, a control head assembly to control the working of the device, an air compressor coupled with the control head assembly to provide compressed air for inflation of tires, a hose assembly to connect the device with the vehicle tires and a plurality of power adapters to connect the device with the power source.

Abstract: This disclosure provides, among other things, a 5? adapter of the formula 3?*—X—(5?5?)—Y—3?, where: 3?* is a blocked 3? end, X is a synthetic sequence, (5?5?) is an internal 5?-5? linkage, Y is an adapter sequence, and 3? is a hydroxylated 3? end. In use, sequence X hybridizes to sequence X? in a population of RNA molecules of formula R—X?, which increases the efficiency of ligation of the 5? adapter to the nucleic acid molecules.

Abstract: One example aspect of the present disclosure relates to a method. The method can include receiving, by one or more computing devices, an input related to power consumption. The method can include filtering, by the one or more computing devices, the received input. The method can include classifying, by the one or more computing devices, the filtered input into one zone of a plurality of zones. The method can include determining, by the one or more computing devices, a setting associated with the classified zone. The setting can determine power production during an idle setting. The method can include causing, by the one or more computing devices, an adjustment to the determined setting at a rate determined by a rate limit.

Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise adding a polyA tail to the longer RNA fragments but not the shorter RNA fragments in a sample by incubating the population of RNA fragments with a polyA polymerase, wherein the reaction conditions used preferentially tail only the longer fragments but not the shorter fragments.

Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise reverse transcribing mRNA to produce DNA:mRNA hybrids, treating the DNA:mRNA hybrids with RNAseH to produce mRNA fragments, and reverse transcribing the mRNA fragments.

Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise adding a polyA tail to the longer RNA fragments but not the shorter RNA fragments in a sample by incubating the population of RNA fragments with a polyA polymerase, wherein the reaction conditions used preferentially tail only the longer fragments but not the shorter fragments.

Abstract: Examples herein involve a document object model (DOM) transaction crawler. Examples herein involve identifying an event in a first state of a document object model. The event is executed to cause a state change from the first state of the document object model to a second state of the document object model. Furthermore, the state change is stored in a transaction log to store the difference between the first state of the document object model and the second state of the document object model.

Abstract: This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types.

Abstract: An Ethernet SAN system includes an initiator device and a target device coupled together by an Ethernet fabric that includes a plurality of Ethernet switch devices. The Ethernet switch devices exchange L2 FIP domain exchange multicast frames that include domain information associated with each of the Ethernet switch devices. That domain information is then used to exchange L2 FIP path exchange multicast frames that include path information associated with each of the Ethernet switch devices. That path information is then used to calculate path costs associated with at least some of the Ethernet switch devices. Those path costs are then used to exchange L2 FIP device exchange unicast frames that include device information associated with the initiator device and the target device. The Ethernet switch devices then transmit data between the initiator device and the target device based on the device information and the path costs.

Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise reverse transcribing mRNA to produce DNA:mRNA hybrids, treating the DNA:mRNA hybrids with RNAseH to produce mRNA fragments, and reverse transcribing the mRNA fragments.