Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise adding a polyA tail to the longer RNA fragments but not the shorter RNA fragments in a sample by incubating the population of RNA fragments with a polyA polymerase, wherein the reaction conditions used preferentially tail only the longer fragments but not the shorter fragments.

Abstract: Systems and techniques for determining and/or generating a navigation path through a three-dimensional (3D) model are presented. At least one waypoint location within a captured 3D model of an architectural environment is determined. A path within the captured 3D model, to navigate between a first location associated with the captured 3D model and a second location associated with the captured 3D model, is determined based on the at least one waypoint location. Visual data indicative of 2D data or 3D data of the captured 3D model along the path is transmitted to a remote client device to simulate navigation of the path within the captured 3D model between the first location and the second location.

Abstract: In one example in accordance with the present disclosure, a method may include receiving an initial document object model (DOM) corresponding to an HTML page structure including a plurality of executable JavaScript code and simulating user interaction with an executable JavaScript code belonging to the plurality in the HTML page structure. The method may include adjusting the initial DOM based on the simulated user interaction, generating byte code corresponding to the adjust DOM and analyzing the byte code using a set of static rules.

Abstract: This disclosure provides, among other things, a method for making a cDNA library. In some embodiments the method may comprise adding a polyA tail to the longer RNA fragments but not the shorter RNA fragments in a sample by incubating the population of RNA fragments with a polyA polymerase, wherein the reaction conditions used preferentially tail only the longer fragments but not the shorter fragments.

Abstract: This disclosure provides, among other things, a 5? adapter of the formula 3?*-X-(5?5?)-Y-3?, where: 3?* is a blocked 3? end, X is a synthetic sequence, (5?5?) is an internal 5?-5? linkage, Y is an adapter sequence, and 3? is a hydroxylated 3? end. In use, sequence X hybridizes to sequence X? in a population of RNA molecules of formula R-X?, which increases the efficiency of ligation of the 5? adapter to the nucleic acid molecules.

Abstract: Bifunctional molecules having first and second alpha-synuclein binding agents coupled by a linker are disclosed. The bifunctional molecules have potential utility in the diagnosis, treatment and/or prophylaxis of disorders in which alpha-synuclein is implicated, including Parkinson's disease. Methods of making and using the bifunctional molecules are disclosed.

Abstract: This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types.

Abstract: Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.

Abstract: Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.

Abstract: Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.

Abstract: Disclosed are devices and methods for transplanting cells, such as pancreatic endoderm cells, into a host. The devices include a non-woven fabric external to a cell-excluding membrane, and the non-woven fabric and/or cell-excluding membrane can be perforated. Treatment of the host with immunosuppressive reagents, required to inhibit allograft rejection due to perforations in the cell delivery device, does not compromise maturation or function of transplanted pancreatic endoderm cells.

Abstract: The present invention is an apparatus for tire inflation designed to allow users to properly inflate, maintain, and monitor the condition of vehicle tires. The tire inflation device of present invention is comprising of: a carrying case having a housing, a lid attached to the housing using a hinge, a plurality of locking latches to lock the lid over the housing and a handle configured over the lid to allow easy transport of the tire inflating device. The tire inflation device further comprises, a control head assembly to control the working of the device, an air compressor coupled with the control head assembly to provide compressed air for inflation of tires, a hose assembly to connect the device with the vehicle tires and a plurality of power adapters to connect the device with the power source.

Abstract: A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

Abstract: A method for software development, installation, and management in a computer system may include: allowing first users to create components of applications; allowing second users, at a display of a web-browser-capable communications device, to build, edit, save, and compile new applications, using the components of the applications as end-user development tools, and to add the new applications to the library of applications; and allowing the second users, at the display of the web-browser-capable communications device, to download one or more of the new applications from the library of applications to mobile communications devices. When executed by the processor, the stored instructions control the computer system to perform functions that may include: connecting the mobile communications devices in a network using the downloaded one or more of the new applications; and causing information transfer to occur within the network that is independent of the processor of the computer system.

Abstract: Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types.

Abstract: In one example in accordance with the present disclosure, a method may include receiving code corresponding to an HTML page structure including a plurality of executable JavaScript code and enumerating a branching path in the JavaScript code. The method may include determining a branch of the branching path that would not execute during normal operation of a browser executing the JavaScript code. The method may also include generating an artificial condition for the branch and executing the branch using the artificial condition.

Abstract: Disclosed herein are universal donor stem cells and cells derived therefrom and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming allogeneic immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor cells disclosed herein are pancreatic endoderm cells that do not express one or more MHC-Class I cell-surface proteins and whose expression of at least one NK activating ligand is disrupted or inhibited.

Abstract: Disclosed herein are universal donor stem cells and cells derived therefrom and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming allogeneic immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor cells disclosed herein are pancreatic endoderm cells that do not express one or more MHC-Class I cell-surface proteins and whose expression of at least one NK activating ligand is disrupted or inhibited.

Abstract: A human immature endocrine cell population and methods for making an immature endocrine cell population are provided. Specifically, immature beta cells and methods for production of immature beta cells are described. Immature beta cells co-express INS and NKX6.1 and are uni-potent and thereby develop into mature beta cells when implanted in vivo. The mature beta cells in vivo are capable of producing insulin in response to glucose stimulation.

Abstract: Embodiments are directed towards push signaling to run jobs on available servers. Users may provide jobs or job instructions to a job control node. Job control node may determine one or more available job target nodes from among a plurality of managed nodes associated with the job control node. The job target nodes may be determined based on information included in the provided job instructions. If there are available job target nodes, the job control node may provide the job instructions to each available job target node. Further, the job control node may receive communications from one or more available job target nodes that indicates that the job target node is prepared and ready to execute the received job instructions. Next, the job control node may issue a job start command that triggers the prepared job target nodes to begin executing the provided job instructions.