Patents by Inventor Kevin T. Chapman
Kevin T. Chapman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11971409Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: GrantFiled: June 4, 2020Date of Patent: April 30, 2024Assignee: Bruker Cellular Analysis, Inc.Inventors: Kevin T. Chapman, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, Jr., Xiao Guan Radstrom, Jason M. McEwen, Gang F. Wang, George L. Fox, Peggy A. Radel
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Publication number: 20240027396Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: ApplicationFiled: May 25, 2023Publication date: January 25, 2024Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
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Patent number: 11666912Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.Type: GrantFiled: January 22, 2019Date of Patent: June 6, 2023Assignee: Berkeley Lights, Inc.Inventors: Kevin D. Loutherback, Yelena Bronevetsky, Peter J. Beemiller, Xiaohua Wang, Kevin T. Chapman
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Publication number: 20220388003Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: ApplicationFiled: March 24, 2022Publication date: December 8, 2022Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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CAPTURING SPECIFIC NUCLEIC ACID MATERIALS FROM INDIVIDUAL BIOLOGICAL CELLS IN A MICRO-FLUIDIC DEVICE
Publication number: 20220379320Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.Type: ApplicationFiled: March 15, 2022Publication date: December 1, 2022Inventors: Kevin T. Chapman, Eric D. Hobbs, Steven W. Short, Mark P. White, Daniele Malleo -
Publication number: 20220356429Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: ApplicationFiled: May 12, 2022Publication date: November 10, 2022Inventors: Randall D. LOWE, JR., Kristin G. BEAUMONT, Aathavan KARUNAKARAN, Natalie C. MARKS, Jason M. MCEWEN, Mark P. WHITE, J. Tanner NEVILL, Gang F. WANG, Andrew W. MCFARLAND, Daniele Malleo, Keith J. BREINLINGER, Xiao GUAN, Kevin T. CHAPMAN
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Patent number: 11365381Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: July 2, 2020Date of Patent: June 21, 2022Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Kristin G. Beaumont, Aathavan Karunakaran, Natalie C. Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
Patent number: 11318479Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.Type: GrantFiled: December 18, 2014Date of Patent: May 3, 2022Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Eric D. Hobbs, Steven W. Short, Mark P. White, Daniele Malleo -
Patent number: 11305283Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: GrantFiled: July 11, 2019Date of Patent: April 19, 2022Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Patent number: 11273177Abstract: The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.Type: GrantFiled: April 14, 2017Date of Patent: March 15, 2022Assignee: Berkeley Lights, Inc.Inventors: Kevin T Chapman, Xiaohua Wang, Xiao Guan Radstrom, Yelena Bronevetsky, Guido K Stadler, Gregory G Lavieu, Annamaria Mocciaro
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Publication number: 20210368781Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.Type: ApplicationFiled: April 12, 2021Publication date: December 2, 2021Applicant: BERKELEY LIGHTS, INC.Inventors: Mark P. White, Kevin T. Chapman, Andrew W. McFarland, Eric D. Hobbs, Randall D. Lowe, JR.
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Publication number: 20210270817Abstract: Disclosed herein is a method for assaying binding affinity between a first molecule and a second molecule in a micro-fluidic device.Type: ApplicationFiled: March 11, 2021Publication date: September 2, 2021Applicant: Berkeley Lights, Inc.Inventors: Paul M. Lebel, Troy A. Lionberger, Kevin T. Chapman
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Patent number: 10973227Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.Type: GrantFiled: April 22, 2016Date of Patent: April 13, 2021Assignee: Berkeley Lights, Inc.Inventors: Mark P. White, Kevin T. Chapman, Andrew W. McFarland, Eric D. Hobbs, Randall D. Lowe, Jr.
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Publication number: 20210087252Abstract: This disclosure relates to the production and use of an isolated, purified and/or recombinant T cell receptor (TCR) that specifically binds to a mutant IDH1 protein, or a fragment thereof, wherein the mutant IDH1 protein or fragment thereof comprises an R132H mutation.Type: ApplicationFiled: August 7, 2020Publication date: March 25, 2021Applicants: Berkeley Lights, Inc., The Regents of the University of CaliforniaInventors: Hideho Okada, Duane Smith, Payal Watchmaker, Yelena Bronevetsky, Ryosuke Naka, Guido K. Stadler, Xiaohua Wang, Kevin T. Chapman
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Publication number: 20210069698Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.Type: ApplicationFiled: July 14, 2020Publication date: March 11, 2021Inventors: Xiao Guan, Mark P. White, Jason M. McEwen, Gang F. Wang, Kevin T. Chapman, Xiaohua Wang, Christine E. Sun
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Publication number: 20210071124Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: ApplicationFiled: July 2, 2020Publication date: March 11, 2021Inventors: Randall D. LOWE, JR., Kristin G. BEAUMONT, Aathavan KARUNAKARAN, Natalie C. MARKS, Jason M. MCEWEN, Mark P. WHITE, J. Tanner NEVILL, Gang F. WANG, Andrew W. MCFARLAND, Daniele Malleo, Keith J. BREINLINGER, Xiao GUAN, Kevin T. CHAPMAN
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Publication number: 20200400669Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: ApplicationFiled: June 4, 2020Publication date: December 24, 2020Applicant: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, JR., Xiao Guan Radstrom, Jason M. McEwen, Gang F. Wang, George L. Fox, Peggy A. Radel
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Publication number: 20200392567Abstract: Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. The methods can include contacting a biological cell with a test agent for a period of time; lysing the biological cell while the biological cell is disposed within a sequestration pen located within an enclosure of a microfluidic device; and allowing RNA molecules released from the lysed biological cell to be captured by capture oligonucleotides linked to a capture object disposed within the sequestration pen of the microfluidic device. Each capture oligonucleotide can include a priming sequence that binds a primer, and a capture sequence. Each cDNA transcribed from a captured RNA can have an oligonucleotide sequence complementary to the captured RNA molecule, with the complementary oligonucleotide sequence being covalently linked to one of the capture oligonucleotides of the capture object.Type: ApplicationFiled: June 24, 2020Publication date: December 17, 2020Applicant: Berkeley Lights, Inc.Inventors: Hayley M. Bennett, Ravi K. Ramenani, Debjit Ray, Thomas M. Vetterli, Annamaria Mocciaro, Magali Soumillon, Mark P. White, Troy A. Lionberger, Kevin T. Chapman, Po-Yuan Tung
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Publication number: 20200299351Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.Type: ApplicationFiled: January 15, 2020Publication date: September 24, 2020Applicant: Berkeley Lights, Inc.Inventors: Peter J. Beemiller, Alexander J. Mastroianni, Shao Ning Pei, Randall D. Lowe, JR., Annamaria Mocciaro, Kevin D. Loutherback, Yelena Bronevetsky, Guido K. Stadler, Andrew W. McFarland, Kevin T. Chapman, Duane Smith, Natalie C. Marks, Amanda L. Goodsell
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Publication number: 20200284756Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: ApplicationFiled: May 7, 2020Publication date: September 10, 2020Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu