Patents by Inventor Kevin T. Chapman
Kevin T. Chapman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10751715Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.Type: GrantFiled: April 22, 2016Date of Patent: August 25, 2020Assignee: Berkeley Lights, Inc.Inventors: Xiao Guan, Mark P. White, Jason M. McEwen, Gang F. Wang, Kevin T. Chapman, Xiaohua Wang, Christine E. Sun
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Patent number: 10723988Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.Type: GrantFiled: April 22, 2016Date of Patent: July 28, 2020Assignee: Berkeley Lights, Inc.Inventors: Randall D. Lowe, Jr., Kristin Beaumont, Aathavan Karunakaran, Natalie Marks, Jason M. McEwen, Mark P. White, J. Tanner Nevill, Gang F. Wang, Andrew W. McFarland, Daniele Malleo, Keith J. Breinlinger, Xiao Guan, Kevin T. Chapman
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Patent number: 10712344Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: GrantFiled: January 13, 2017Date of Patent: July 14, 2020Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, George L. Fox, Peggy A. Radel, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, Jr., Xiao Guan Radstrom, Jason M. McEwen, Gang F. Wang
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Patent number: 10690628Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: GrantFiled: December 14, 2017Date of Patent: June 23, 2020Assignee: Berkeley Lights, IncInventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
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Publication number: 20200139362Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.Type: ApplicationFiled: October 17, 2019Publication date: May 7, 2020Applicant: Berkeley Lights, Inc.Inventors: Peter J. BEEMILLER, Alexander J. MASTROIANNI, Shao Ning PEI, Randall D. LOWE, Jr., Annamaria MOCCIARO, Kevin D. LOUTHERBACK, Yelena BRONEVETSKY, Guido K. STADLER, Andrew W. MCFARLAND, Kevin T. CHAPMAN, Duane SMITH, Natalie C. MARKS, Amanda L. GOODSELL
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Publication number: 20200115680Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.Type: ApplicationFiled: July 19, 2019Publication date: April 16, 2020Applicant: Berkeley Lights, Inc.Inventors: Yelena Bronevetsky, Xiaohua Wang, Peter J. Beemiller, Kristin G. Beaumont, Randall D. Lowe, JR., Alexander J. Mastroianni, Kevin T. Chapman, Natalie C. Marks
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Publication number: 20200078788Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: ApplicationFiled: July 11, 2019Publication date: March 12, 2020Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Publication number: 20200064337Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.Type: ApplicationFiled: April 22, 2019Publication date: February 27, 2020Inventors: Minha Park, Jason C. Briggs, Jason M. McEwen, Ravi K. Ramenani, Hariharasudhan Chirra Dinakar, Kai W. Szeto, Adrienne T. Higa, Mark P. White, Randall D. Lowe, JR., Xiaohua Wang, Kevin T. Chapman
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Publication number: 20190283026Abstract: Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (Dc) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.Type: ApplicationFiled: January 22, 2019Publication date: September 19, 2019Inventors: Kevin D. Loutherback, Yelena Bronevetsky, Peter J. Beemiller, Xiaohua Wang, Kevin T. Chapman
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Patent number: 10376886Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: GrantFiled: December 15, 2017Date of Patent: August 13, 2019Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Publication number: 20180272350Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: ApplicationFiled: December 15, 2017Publication date: September 27, 2018Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M. Jimena Loureiro
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Publication number: 20180259482Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: ApplicationFiled: December 14, 2017Publication date: September 13, 2018Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
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Publication number: 20180135011Abstract: Methods of expanding T lymphocytes in a microfluidic device are provided. The methods can include introducing one or more T lymphocytes into a microfluidic device; contacting the one or more T lymphocytes with an activating agent; and perfusing culture medium through the microfluidic device for a period of time sufficient to allow the one or more T lymphocytes to undergo at least one round of mitotic cell division. The expansion can be non-specific or antigen-specific. T lymphocytes produced according to the disclosed methods are also provided, along with methods of treating cancer in a subject. The methods of treating cancer can include isolating T lymphocytes from a tissue sample obtained from the subject; expanding the isolated T lymphocytes in a microfluidic device; exporting the expanded T lymphocytes from the microfluidic device; and reintroducing the expanded T lymphocytes into the subject.Type: ApplicationFiled: November 2, 2017Publication date: May 17, 2018Inventors: Yelena Bronevetsky, Xiaohua Wang, Peter J. Beemiller, Kristin G. Beaumont, Randall D. Lowe, JR., Alexander J. Mastroianni, Kevin T. Chapman
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Patent number: 9889445Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.Type: GrantFiled: October 22, 2014Date of Patent: February 13, 2018Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Daniele Malleo, J. Tanner Nevill, Steven W. Short, Mark P. White, M Jimena Loureiro
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Patent number: 9857333Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.Type: GrantFiled: October 22, 2013Date of Patent: January 2, 2018Assignee: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
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Publication number: 20170276679Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.Type: ApplicationFiled: January 13, 2017Publication date: September 28, 2017Inventors: Kevin T. Chapman, Mark P. White, Xiaohua Wang, Minha Park, Guido K. Stadler, Randall D. Lowe, Jr., Xiao Guan, Jason M. McEwen, Gang Wang, George L. Fox, Peggy A. Radel
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Publication number: 20170224734Abstract: The present disclosure provides methods of preparing tumor infiltrating cells engineered to express a pro-inflammatory polypeptide. The pro-inflammatory polypeptide is expressed from the tumor infiltrating cell to counter a generally immunosuppressive state in and around tumors resulting from an imbalance between the number and activation state of immune effector cells versus those of suppressor cells. Delivering the proinflammatory polypeptide via expression from the TICs, as distinct from systemic administration, reduces side effects from increased inflammation at sides remote from a tumor to be treated.Type: ApplicationFiled: April 14, 2017Publication date: August 10, 2017Inventors: Kevin T Chapman, Xiaohua Wang, Xiao Guan Radstrom, Yelena Bronevetsky, Guido K Stadler, Gregory G Levieu, Annamaria Mocciaro
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Patent number: 9588117Abstract: In some cases, the described systems and methods include obtaining a cell sample containing multiple antibody-producing cells. In such cases, the cells can be tagged with a cross-linking reagent having a first portion configured to bind to a marker on the antibody-producing cells and a second portion configured to bind to an antigen of interest. In some instances, the tagged antibody-producing cells are exposed to the antigen of interest such that the antigen becomes bound to the cells. In some such instances, the antibody-producing cells are also allowed to produce an antibody, such that a portion of the antibody-producing cells produce an antigen-specific antibody that binds to the antigen of interest. To identify cells that produce the antigen-specific antibody, the tagged cells can be exposed to a labeled secondary antibody that is configured to bind to the antigen-specific antibody. Other implementations are also described.Type: GrantFiled: December 26, 2014Date of Patent: March 7, 2017Assignee: Berkeley Lights, Inc.Inventor: Kevin T. Chapman
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CAPTURING SPECIFIC NUCLEIC ACID MATERIALS FROM INDIVIDUAL BIOLOGICAL CELLS IN A MICRO-FLUIDIC DEVICE
Publication number: 20170021366Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.Type: ApplicationFiled: December 18, 2014Publication date: January 26, 2017Applicant: Berkeley Lights, Inc.Inventors: Kevin T. Chapman, Eric D. Hobbs, Steven W. Short, Mark P. White, Daniele Malleo -
Publication number: 20160338347Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.Type: ApplicationFiled: April 22, 2016Publication date: November 24, 2016Inventors: Mark P. White, Kevin T. Chapman, Andrew W. McFarland, Eric D. Hobbs, Randall D. Lowe, JR.