Abstract: The present invention relates to purified and concentrated human milk oligosaccharide compositions and methods of making and using the same.

Abstract: An IC device may include a CMOS layer and memory layers at the frontside and backside of the CMOS layer. The CMOS layer may include one or more logic circuits with MOSFET transistors. The CMOS layer may also include memory cells, e.g., SRAM cells. A memory layer may include one or more memory arrays. A memory array may include memory cells (e.g., DRAM cells), bit lines, and word lines. A logic circuit in the CMOS layer may control access to the memory cells. A memory layer may be bonded with the CMOS layer through a bonding layer that includes conductive structures coupled to a logic circuit in the CMOS layer or to bit lines or word lines in the memory layer. An additional conductive structure may be at the backside of a MOSFET transistor in the CMOS layer and coupled to a conductive structure in the bonding layer.

Abstract: Disclosed is a computer-implemented method of training a likelihood-based computational model for determining the position of an image representation of an annotated anatomical structure in a two-dimensional x-ray image, wherein the method encompasses inputting medical DRRs together with annotation to a machine learning algorithm to train the algorithm, i.e. to generate adapted learnable parameters of the machine learning model. The annotations may be derived from metadata associated with the DRRs or may be included in atlas data which is matched with the DRRs to establish a relation between the annotations included in the atlas data and the DRRs. The thus generated machine learning algorithm may then be used to analyse clinical or synthesized DRRs so as to appropriately add annotations to those DRRs and/or identify the position of an anatomical structure in those DRRs.

Abstract: Disclosed is a computer-implemented method of training a likelihood-based computational model for determining the position of an image representation of an annotated anatomical structure in a two-dimensional x-ray image, wherein the method encompasses inputting medical DRRs together with annotation to a machine learning algorithm to train the algorithm, i.e. to generate adapted leamable parameters of the machine learning model. The annotations may be derived from metadata associated with the DRRs or may be included in atlas data which is matched with the DRRs to establish a relation between the annotations included in the atlas data and the DRRs. The thus generated machine learning algorithm may then be used to analyse clinical or synthesized DRRs so as to appropriately add annotations to those DRRs and/or identify the position of an anatomical structure in those DRRs.

Abstract: The present invention relates to methods for reducing low molecular weight species of recombinantly-produced proteins. In particular, methods of reducing the formation of low molecular weight species produced by a host cell during the cell culture process through pH control of the production cell culture are disclosed. Also disclosed are methods for reducing or eliminating the generation of alternative splice variants by a host cell during production of a recombinant protein.

Abstract: A method of implanting a prosthetic heart valve includes advancing a distal end portion of a catheter shaft through a patient's vasculature. The distal end portion of the catheter shaft includes an expansion device. A prosthetic heart valve is mounted on the expansion device with the expansion device and the prosthetic heart valve in a compressed configuration. The method further includes positioning the distal end portion of the catheter shaft and the prosthetic heart valve to an implantation location and expanding the prosthetic heart valve and the expansion device to an expanded configuration. The expansion device includes a main body and a plurality of projections extending radially from the main body. The projections are spaced apart relative to each such that there are grooves extending between adjacent projections providing perfusion passageways between the expansion device and the prosthetic heart valve when the expansion device is in the expanded configuration.

Abstract: A method of implanting a prosthetic heart valve includes advancing a distal end portion of a catheter shaft through a patient's vasculature. The distal end portion of the catheter shaft includes an expansion device. A prosthetic heart valve is mounted on the expansion device with the expansion device and the prosthetic heart valve in a compressed configuration. The method further includes positioning the distal end portion of the catheter shaft and the prosthetic heart valve to an implantation location, and expanding the prosthetic heart valve and the expansion device from the compressed configuration to an expanded configuration. The expansion device includes an inner expandable member and a plurality of outer expandable members. The outer expandable members are distributed such that there are gaps providing perfusion passageways between the expansion device and the prosthetic heart valve when the expansion device is in the expanded configuration.

Abstract: A circuit arrangement for switching an electrical load comprising an electrical switching element with a control input and an output; a control unit connected to the control input to drive the electrical switching element, in a first switching state, to generate a first output signal forming a switch-on signal for the load and, in a second switching state, to generate a second output signal, smaller than the first output signal, forming a switch-off signal for the electrical load; a safety output connected electrically to the electrical switching element output and to which the electrical load is connected; a decoupling apparatus arranged between the electrical switching element output and the safety output; a first output signal detecting apparatus connected to the electrical switching element output; and a second output signal detecting apparatus arranged in a circuit path between the decoupling apparatus and the safety output.

Abstract: The present invention relates to device for automated synthesis of oligo- and polysaccharides on a solid support.

Abstract: The present invention generally relates to automated synthesis technology, and more particularly, to a device and method for automated synthesis of oligo- and polysaccharides on a solid support. In particular the present invention relates to a device for automated synthesis of oligo- and polysaccharides on a solid support comprising a reaction vessel, a reagent storing component, a reagent delivery system, a cooling device for cooling reaction vessel, and a pre-cooling device for pre-cooling the reagents to be supplied.

Abstract: The present invention relates to a method for synthesizing oligo- and polysaccharides using microwave radiation, in particular to a method and a device for automated synthesis of oligo- and polysaccharides.

Abstract: The invention relates to a compound of formula Ia and/or formula Ib: or a pharmaceutically acceptable salt, solvate or prodrug thereof, where the groups are defined herein. The invention also relates to a pharmaceutical formulation comprising the compound for treating or detecting a microbial infection in a subject, a method of determining antimicrobial resistance of a microbial infection using the compound, and a method of determining an effective dose of one or more antimicrobial agents to kill a microorganism using the compound.

Abstract: A recombinant vector according to an embodiment of the present invention may be used for producing a reconstituted cytochrome P450 oxygenase-reductase fusion protein. Oxygenase and reductase may be independently expressed in a host cell into which the recombinant vector is introduced, and then may be fused with a split intein so that it is possible to increase the heme content contained in the active site of the oxygenase and improve enzyme activity and stability.

Abstract: Disclosed is a computer-implemented method of training a likelihood-based computational model for determining the position of an image representation of an annotated anatomical structure in a two-dimensional x-ray image, wherein the method encompasses inputting medical DRRs together with annotation to a machine learning algorithm to train the algorithm, i.e. to generate adapted leamable parameters of the machine learning model. The annotations may be derived from metadata associated with the DRRs or may be included in atlas data which is matched with the DRRs to establish a relation between the annotations included in the atlas data and the DRRs. The thus generated machine learning algorithm may then be used to analyse clinical or synthesized DRRs so as to appropriately add annotations to those DRRs and/or identify the position of an anatomical structure in those DRRs.

Abstract: A recombinant vector according to an embodiment of the present invention may be used for producing a reconstituted cytochrome P450 oxygenase-reductase fusion protein. Oxygenase and reductase may be independently expressed in a host cell into which the recombinant vector is introduced, and then may be fused with a split intein so that it is possible to increase the heme content contained in the active site of the oxygenase and improve enzyme activity and stability.

Abstract: A circuit arrangement for switching an electrical load comprising an electrical switching element with a control input and an output; a control unit connected to the control input to drive the electrical switching element, in a first switching state, to generate a first output signal forming a switch-on signal for the load and, in a second switching state, to generate a second output signal, smaller than the first output signal, forming a switch-off signal for the electrical load; a safety output connected electrically to the electrical switching element output and to which the electrical load is connected; a decoupling apparatus arranged between the electrical switching element output and the safety output; a first output signal detecting apparatus connected to the electrical switching element output; and a second output signal detecting apparatus arranged in a circuit path between the decoupling apparatus and the safety output.

Abstract: A method of implanting a prosthetic heart valve includes advancing a distal end portion of a catheter shaft through a patient's vasculature. The distal end portion of the catheter shaft includes an expansion device. A prosthetic heart valve is mounted on the expansion device with the expansion device and the prosthetic heart valve in a compressed configuration. The method further includes positioning the distal end portion of the catheter shaft and the prosthetic heart valve to an implantation location, and expanding the prosthetic heart valve and the expansion device from the compressed configuration to an expanded configuration. The expansion device includes an inner expandable member and a plurality of outer expandable members. The outer expandable members are distributed such that there are gaps providing perfusion passageways between the expansion device and the prosthetic heart valve when the expansion device is in the expanded configuration.

Abstract: A delivery system for stabilizing a catheter shaft across an aortic arch can include one or more stabilizing members configured to fix or stabilize the position of the catheter relative to the aortic arch of a patient.

Abstract: A safety switching device for an automated installation includes a power supply unit for providing at least one operating voltage for the device. The power supply unit receives an input voltage via a feed line and provides a defined output voltage on an output side. Electrical supply to the power supply unit is interrupted if an instantaneous output voltage is higher than the defined output voltage. The interruption is achieved by first and second switching elements arranged in series and connecting the input of the power supply unit to ground, and by an interrupter element in the feed line. The interrupter element triggers when both the first and the second switching elements are in a closed, low-impedance state.

Abstract: A safety switching apparatus for switching on or off a technical installation has a failsafe control/evaluation unit with an input for receiving an input signal. The failsafe control/evaluation unit is designed to process the input signal in order to produce an output signal for switching on or off the technical installation at a defined output signal time. The failsafe control/evaluation unit has a first output for transmitting the output signal to an electromechanical switch. The electromechanical switch has an operating contact for switching a load circuit of the technical installation and has a positively guided auxiliary contact in an auxiliary contact current path. The auxiliary contact can be used to carry a current for checking the switching position of the operating contact. A switching element is arranged in the auxiliary contact current path.