Patents by Inventor Kirk M. Ririe

Kirk M. Ririe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8343754
    Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: January 1, 2013
    Assignees: University of Utah Research Foundation, BioFire Diagnostics, Inc.
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Publication number: 20120258524
    Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
    Type: Application
    Filed: May 7, 2012
    Publication date: October 11, 2012
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Patent number: 7745205
    Abstract: A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in containers each comprising a reservoir and a reaction portion, wherein the reaction portion has a small volume. The small volume reaction portion permits the rapid and accurate temperature modulation. With an optically transmissible reaction portion, DNA amplification may be monitored by fluorescence during PCR.
    Type: Grant
    Filed: August 9, 2004
    Date of Patent: June 29, 2010
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Kirk M. Ririe
  • Publication number: 20100105029
    Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.
    Type: Application
    Filed: May 8, 2006
    Publication date: April 29, 2010
    Inventors: Kirk M. Ririe, Michael R. Newswander, Randy P. Rasmussen, Mark Aaron Poritz, Stewart Benjamin Smith, David E. Jones, Gary Clark Kessler
  • Publication number: 20100056383
    Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.
    Type: Application
    Filed: November 14, 2007
    Publication date: March 4, 2010
    Inventors: Kirk M. Ririe, Gary Clark Kessler, David E. Jones, Mark Aaron` Poritz
  • Patent number: 7670832
    Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
    Type: Grant
    Filed: August 15, 2005
    Date of Patent: March 2, 2010
    Assignees: University of Utah Research Foundation, Idaho Technology, Inc.
    Inventors: Carl T. Wittwer, Kirk M. Ririe
  • Publication number: 20090311673
    Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
    Type: Application
    Filed: October 31, 2007
    Publication date: December 17, 2009
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Publication number: 20090258414
    Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
    Type: Application
    Filed: October 29, 2007
    Publication date: October 15, 2009
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Patent number: 7273749
    Abstract: A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in containers each comprising a reservoir and a reaction portion, wherein the reaction portion has a small volume. The small volume reaction portion permits the rapid and accurate temperature modulation. With an optically transmissible reaction portion, DNA amplification may be monitored by fluorescence during PCR.
    Type: Grant
    Filed: August 3, 2000
    Date of Patent: September 25, 2007
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Kirk M. Ririe
  • Patent number: 7081226
    Abstract: A thermal cycling method and device is disclosed. The device comprises a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such as the DNA polymerase chain reaction. Biological samples are placed in glass micro capillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Monitoring of the DNA amplification is monitored by fluorescence once per cycle or many times per cycle. The present invention provides that fluorescence monitoring of PCR is a powerful tool for DNA quantification.
    Type: Grant
    Filed: June 4, 1997
    Date of Patent: July 25, 2006
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen, David R. Hillyard
  • Patent number: 6569627
    Abstract: The present invention is directed to a method and kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to a method wherein the nucleic acid is monitored during polymerase chain reaction using a double-stranded nucleic acid binding dye capable of producing a fluorescent signal related to the amount of the nucleic acid present in a sample, wherien the dye is selected from the group consisting of SYBR™ Green I and pico green.
    Type: Grant
    Filed: March 5, 2001
    Date of Patent: May 27, 2003
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Publication number: 20020058258
    Abstract: The present invention is directed to a method and kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to a method wherein the nucleic acid is monitored during polymerase chain reaction using a heat-labile double-stranded nucleic acid binding dye capable of producing a fluorescent signal related to the amount of the nucleic acid present in a sample.
    Type: Application
    Filed: March 5, 2001
    Publication date: May 16, 2002
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Patent number: 6232079
    Abstract: The present invention is directed to a method of determining the concentration of a nucleic acid product that had been amplified through polymerase chain reaction (PCR). More particularly, the present invention relates to a method wherein a rate constant is determined for a known concentration of amplified product by monitoring the rate of hybridization of the known concentration, and then the concentration of an unknown concentration of a nucleic acid product can be determined by determining the rate of annealing for the unknown concentration, and calculating the concentration from the rate of annealing and the rate constant.
    Type: Grant
    Filed: August 9, 2000
    Date of Patent: May 15, 2001
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Patent number: 6174670
    Abstract: Methods of monitoring hybridization during polymerase chain reaction are disclosed. These methods are achieved with rapid thermal cycling and use of double stranded DNA dyes or specific hybridization probes. A fluorescence resonance energy transfer pair comprises fluorescein and Cy5 or Cy5.5. Methods for quantitating amplified DNA and determining its purity are carried out by analysis of melting and reannealing curves.
    Type: Grant
    Filed: June 4, 1997
    Date of Patent: January 16, 2001
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, Kirk M. Ririe, Randy P. Rasmussen
  • Patent number: 5455175
    Abstract: A thermal cycling device having a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass microcapillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achievable by the present invention.
    Type: Grant
    Filed: January 10, 1994
    Date of Patent: October 3, 1995
    Assignee: University of Utah Research Foundation
    Inventors: Carl T. Wittwer, David R. Hillyard, Kirk M. Ririe