Patents by Inventor Kiyotoshi Sekiguchi
Kiyotoshi Sekiguchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11959103Abstract: Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.Type: GrantFiled: November 9, 2017Date of Patent: April 16, 2024Assignees: OSAKA UNIVERSITY, KYOTO UNIVERSITYInventors: Kiyotoshi Sekiguchi, Fumi Ebisu, Hidetoshi Sakurai, Mingming Zhao, Megumu Saito
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Publication number: 20230203131Abstract: The present invention provides a gel formed of fibrin and a molecule generated by thrombin treatment of a chimeric protein that comprises a fibrinogen fragment capable of binding to fibrinogen upon thrombin treatment and a laminin fragment having integrin-binding activity, and optionally further comprises a protein having growth factor-binding activity. The gel of the present invention is suitable as a gel substrate that has properties of the basement membrane and can be used in medical applications.Type: ApplicationFiled: May 7, 2021Publication date: June 29, 2023Applicants: OSAKA UNIVERSITY, MATRIXOME, INC.Inventors: Kiyotoshi SEKIGUCHI, Mamoru TAKIZAWA, Yukimasa TANIGUCHI
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Patent number: 11649433Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.Type: GrantFiled: January 31, 2018Date of Patent: May 16, 2023Assignee: Osaka UniversityInventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
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Publication number: 20230114089Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF? inhibitor, SHH signal-stimulating agent, FGF8, and GSK3? inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.Type: ApplicationFiled: May 25, 2022Publication date: April 13, 2023Applicants: KYOTO UNIVERSITY, OSAKA UNIVERSITY, EISAI R&D MANAGEMENT CO., LTD.Inventors: Jun Takahashi, Daisuke Doi, Bumpei Samata, Kiyotoshi Sekiguchi, Yuichi Ono
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Patent number: 11492593Abstract: The present invention provides a method of purifying highly pure retinal pigment epithelial cells from a cell population obtained by induction of differentiation of pluripotent stem cells into retinal pigment epithelial cells, by a simple and easy operation in a short period. The purification method of the present invention includes a step of introducing a cell population containing retinal pigment epithelial cells obtained by differentiation induction of pluripotent stem cells on laminin or a fragment thereof on a filter, and obtaining a cell population that passed the filter.Type: GrantFiled: October 9, 2014Date of Patent: November 8, 2022Assignees: HEALIOS K.K., OSAKA UNIVERSITYInventors: Masanori Sawada, Kiyotoshi Sekiguchi
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Publication number: 20220340868Abstract: The present invention relates to improvement of the yield of skin-derived pluripotent precursor cells in induction of differentiation of stem cells to skin-derived pluripotent precursor cells. The present invention provides a method for preparing skin-derived pluripotent precursor cell comprising culturing a neural crest stem cells in the presence of at least one selected from the group consisting of laminin and a fragment thereof to differentiate the cells to skin-derived pluripotent precursor cells, wherein the laminin is at least one selected from the group consisting of laminin 111, laminin 121, laminin 332, laminin 421, laminin 511, laminin 521, and a variant thereof.Type: ApplicationFiled: August 4, 2020Publication date: October 27, 2022Applicants: KAO CORPORATION, OSAKA UNIVERSITYInventors: Yoriko NAKAGIRI, Kiyotoshi SEKIGUCHI
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Patent number: 11473058Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF? inhibitor, SHH signal-stimulating agent, FGF8, and GSK3? inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.Type: GrantFiled: September 4, 2014Date of Patent: October 18, 2022Assignees: Kyoto University, Osaka University, Eisai R&D Management Co., Ltd.Inventors: Jun Takahashi, Daisuke Doi, Bumpei Samata, Kiyotoshi Sekiguchi, Yuichi Ono
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Publication number: 20220298474Abstract: Provided is a method for efficiently culturing dermal papilla cells while maintaining an ability to induce hair follicles. A method for culturing dermal papilla cells in the presence of at least one selected from the group consisting of EMILIN and a fragment thereof.Type: ApplicationFiled: August 6, 2020Publication date: September 22, 2022Applicants: KAO CORPORATION, OSAKA UNIVERSITYInventors: Shiho YAMASHITA, Yoriko NAKAGIRI, Kiyotoshi SEKIGUCHI, Chisei SHIMONO
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Patent number: 11427806Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF? inhibitor, SHH signal-stimulating agent, FGF8, and GSK3? inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.Type: GrantFiled: September 4, 2014Date of Patent: August 30, 2022Assignees: Kyoto University, Osaka University, Eisai R&D Management Co., Ltd.Inventors: Jun Takahashi, Daisuke Doi, Bumpei Samata, Kiyotoshi Sekiguchi, Yuichi Ono
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Patent number: 11085916Abstract: A method for observing the dynamics of sweat glands and a method for evaluating a substance of interest, which are useful for development of a preparation for external application, such as a cosmetic. In each of the methods, an observation sample is used, which is prepared by staining all sweat glands, which are isolated alive, with a staining reagent, and then holding the all sweat glands on a support using at least one material selected from the group consisting of collagen, agarose, basement membrane matrix, poly-D-lysine and a membrane.Type: GrantFiled: July 18, 2017Date of Patent: August 10, 2021Assignees: MANDOM CORPORATION, OSAKA UNIVERSITYInventors: Kie Nakashima, Ryuichiro Kurata, Fumitaka Fujita, Kiyotoshi Sekiguchi, Atsushi Tanemura, Hiroyuki Murota, Ichiro Katayama
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Patent number: 11060061Abstract: An immortalized sweat gland myoepithelial cell which expresses ?-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.Type: GrantFiled: August 7, 2018Date of Patent: July 13, 2021Assignees: MANDOM CORPORATION, OSAKA UNIVERSITYInventors: Tomohisa Hayakawa, Ryuichiro Kurata, Fumitaka Fujita, Fumihiro Okada, Kiyotoshi Sekiguchi
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Publication number: 20200385680Abstract: An immortalized sweat gland myoepithelial cell which expresses ?-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.Type: ApplicationFiled: August 7, 2018Publication date: December 10, 2020Applicants: MANDOM CORPORATION, OSAKA UNIVERSITYInventors: Tomohisa Hayakawa, Ryuichiro Kurata, Fumitaka Fujita, Fumihiro Okada, Kiyotoshi Sekiguchi
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Patent number: 10696948Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin ?2?1?1, laminin ?2?2?1, laminin ?1?1?1 and laminin ?1?2?1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.Type: GrantFiled: September 14, 2015Date of Patent: June 30, 2020Assignee: OSAKA UNIVERSITYInventors: Yukiko Ochi, Kiyotoshi Sekiguchi, Shigeru Miyagawa, Yoshiki Sawa, Antti Markus Siltanen
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Patent number: 10669529Abstract: Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.Type: GrantFiled: July 14, 2016Date of Patent: June 2, 2020Assignees: Kyoto University, Osaka UniversityInventors: Tatsutoshi Nakahata, Megumu Saito, Akira Niwa, Ryo Ota, Kiyotoshi Sekiguchi
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Publication number: 20200010800Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.Type: ApplicationFiled: January 31, 2018Publication date: January 9, 2020Inventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
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Patent number: 10428311Abstract: The present invention provides a novel technique in a cell culture method using a cell culture vessel coated with a laminin fragment, which novel technique achieves cell culture as in the case of using a recommended coating concentration even when the coating concentration is lower than the recommended coating concentration. The present invention relates to a method for enhancing an activity for mammalian cultured cells of a laminin fragment or a variant thereof each having integrin binding activity.Type: GrantFiled: July 15, 2015Date of Patent: October 1, 2019Assignee: OSAKA UNIVERSITYInventors: Kiyotoshi Sekiguchi, Ko Tsutsui
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Publication number: 20190276804Abstract: Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.Type: ApplicationFiled: November 9, 2017Publication date: September 12, 2019Applicants: OSAKA UNIVERSITY, KYOTO UNIVERSITYInventors: Kiyotoshi SEKIGUCHI, Fumi EBISU, Hidetoshi SAKURAI, Mingming ZHAO, Megumu SAITO
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Publication number: 20190211305Abstract: The present invention provides a method for culturing pluripotent stem cells, comprising the step of contacting pluripotent stem cells with laminin 421 or a fragment thereof, laminin 121 or a fragment thereof, or a combination thereof.Type: ApplicationFiled: August 25, 2017Publication date: July 11, 2019Inventors: Koji Eto, Sou Nakamura, Kiyotoshi Sekiguchi, Tomohiro Shigemori
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Publication number: 20190153391Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin ?2?1?1, laminin ?2?2?1, laminin ?1?1?1 and laminin ?1?2?1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.Type: ApplicationFiled: September 14, 2015Publication date: May 23, 2019Applicant: OSAKA UNIVERSITYInventors: Yukiko OCHI, Kiyotoshi SEKIGUCHI, Shigeru MIYAGAWA, Yoshiki SAWA, Antti Markus SILTANEN
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Patent number: 10287541Abstract: Provided is a cell culture vessel characterized in that a surface to be in contact with cells is coated with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in a dry state, the laminin fragment being derived from at least one kind selected from laminin ?5?1?1 and laminin ?5?2?1, the cell culture vessel being any of the following: (1) a cell culture vessel of which a surface to be in contact with cells is coated only with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in a dry state; (2) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in combination with a laminin fragment having no integrin ?6?1 binding activity in a dry state; and (3) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in combinatioType: GrantFiled: May 9, 2014Date of Patent: May 14, 2019Assignee: OSAKA UNIVERSITYInventors: Kiyotoshi Sekiguchi, Ko Tsutsui