Patents by Inventor Kiyotoshi Sekiguchi

Kiyotoshi Sekiguchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11492593
    Abstract: The present invention provides a method of purifying highly pure retinal pigment epithelial cells from a cell population obtained by induction of differentiation of pluripotent stem cells into retinal pigment epithelial cells, by a simple and easy operation in a short period. The purification method of the present invention includes a step of introducing a cell population containing retinal pigment epithelial cells obtained by differentiation induction of pluripotent stem cells on laminin or a fragment thereof on a filter, and obtaining a cell population that passed the filter.
    Type: Grant
    Filed: October 9, 2014
    Date of Patent: November 8, 2022
    Assignees: HEALIOS K.K., OSAKA UNIVERSITY
    Inventors: Masanori Sawada, Kiyotoshi Sekiguchi
  • Publication number: 20220340868
    Abstract: The present invention relates to improvement of the yield of skin-derived pluripotent precursor cells in induction of differentiation of stem cells to skin-derived pluripotent precursor cells. The present invention provides a method for preparing skin-derived pluripotent precursor cell comprising culturing a neural crest stem cells in the presence of at least one selected from the group consisting of laminin and a fragment thereof to differentiate the cells to skin-derived pluripotent precursor cells, wherein the laminin is at least one selected from the group consisting of laminin 111, laminin 121, laminin 332, laminin 421, laminin 511, laminin 521, and a variant thereof.
    Type: Application
    Filed: August 4, 2020
    Publication date: October 27, 2022
    Applicants: KAO CORPORATION, OSAKA UNIVERSITY
    Inventors: Yoriko NAKAGIRI, Kiyotoshi SEKIGUCHI
  • Patent number: 11473058
    Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF? inhibitor, SHH signal-stimulating agent, FGF8, and GSK3? inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
    Type: Grant
    Filed: September 4, 2014
    Date of Patent: October 18, 2022
    Assignees: Kyoto University, Osaka University, Eisai R&D Management Co., Ltd.
    Inventors: Jun Takahashi, Daisuke Doi, Bumpei Samata, Kiyotoshi Sekiguchi, Yuichi Ono
  • Publication number: 20220298474
    Abstract: Provided is a method for efficiently culturing dermal papilla cells while maintaining an ability to induce hair follicles. A method for culturing dermal papilla cells in the presence of at least one selected from the group consisting of EMILIN and a fragment thereof.
    Type: Application
    Filed: August 6, 2020
    Publication date: September 22, 2022
    Applicants: KAO CORPORATION, OSAKA UNIVERSITY
    Inventors: Shiho YAMASHITA, Yoriko NAKAGIRI, Kiyotoshi SEKIGUCHI, Chisei SHIMONO
  • Patent number: 11427806
    Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF? inhibitor, SHH signal-stimulating agent, FGF8, and GSK3? inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
    Type: Grant
    Filed: September 4, 2014
    Date of Patent: August 30, 2022
    Assignees: Kyoto University, Osaka University, Eisai R&D Management Co., Ltd.
    Inventors: Jun Takahashi, Daisuke Doi, Bumpei Samata, Kiyotoshi Sekiguchi, Yuichi Ono
  • Patent number: 11085916
    Abstract: A method for observing the dynamics of sweat glands and a method for evaluating a substance of interest, which are useful for development of a preparation for external application, such as a cosmetic. In each of the methods, an observation sample is used, which is prepared by staining all sweat glands, which are isolated alive, with a staining reagent, and then holding the all sweat glands on a support using at least one material selected from the group consisting of collagen, agarose, basement membrane matrix, poly-D-lysine and a membrane.
    Type: Grant
    Filed: July 18, 2017
    Date of Patent: August 10, 2021
    Assignees: MANDOM CORPORATION, OSAKA UNIVERSITY
    Inventors: Kie Nakashima, Ryuichiro Kurata, Fumitaka Fujita, Kiyotoshi Sekiguchi, Atsushi Tanemura, Hiroyuki Murota, Ichiro Katayama
  • Patent number: 11060061
    Abstract: An immortalized sweat gland myoepithelial cell which expresses ?-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.
    Type: Grant
    Filed: August 7, 2018
    Date of Patent: July 13, 2021
    Assignees: MANDOM CORPORATION, OSAKA UNIVERSITY
    Inventors: Tomohisa Hayakawa, Ryuichiro Kurata, Fumitaka Fujita, Fumihiro Okada, Kiyotoshi Sekiguchi
  • Publication number: 20200385680
    Abstract: An immortalized sweat gland myoepithelial cell which expresses ?-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.
    Type: Application
    Filed: August 7, 2018
    Publication date: December 10, 2020
    Applicants: MANDOM CORPORATION, OSAKA UNIVERSITY
    Inventors: Tomohisa Hayakawa, Ryuichiro Kurata, Fumitaka Fujita, Fumihiro Okada, Kiyotoshi Sekiguchi
  • Patent number: 10696948
    Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin ?2?1?1, laminin ?2?2?1, laminin ?1?1?1 and laminin ?1?2?1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: June 30, 2020
    Assignee: OSAKA UNIVERSITY
    Inventors: Yukiko Ochi, Kiyotoshi Sekiguchi, Shigeru Miyagawa, Yoshiki Sawa, Antti Markus Siltanen
  • Patent number: 10669529
    Abstract: Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: June 2, 2020
    Assignees: Kyoto University, Osaka University
    Inventors: Tatsutoshi Nakahata, Megumu Saito, Akira Niwa, Ryo Ota, Kiyotoshi Sekiguchi
  • Publication number: 20200010800
    Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
    Type: Application
    Filed: January 31, 2018
    Publication date: January 9, 2020
    Inventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
  • Patent number: 10428311
    Abstract: The present invention provides a novel technique in a cell culture method using a cell culture vessel coated with a laminin fragment, which novel technique achieves cell culture as in the case of using a recommended coating concentration even when the coating concentration is lower than the recommended coating concentration. The present invention relates to a method for enhancing an activity for mammalian cultured cells of a laminin fragment or a variant thereof each having integrin binding activity.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: October 1, 2019
    Assignee: OSAKA UNIVERSITY
    Inventors: Kiyotoshi Sekiguchi, Ko Tsutsui
  • Publication number: 20190276804
    Abstract: Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.
    Type: Application
    Filed: November 9, 2017
    Publication date: September 12, 2019
    Applicants: OSAKA UNIVERSITY, KYOTO UNIVERSITY
    Inventors: Kiyotoshi SEKIGUCHI, Fumi EBISU, Hidetoshi SAKURAI, Mingming ZHAO, Megumu SAITO
  • Publication number: 20190211305
    Abstract: The present invention provides a method for culturing pluripotent stem cells, comprising the step of contacting pluripotent stem cells with laminin 421 or a fragment thereof, laminin 121 or a fragment thereof, or a combination thereof.
    Type: Application
    Filed: August 25, 2017
    Publication date: July 11, 2019
    Inventors: Koji Eto, Sou Nakamura, Kiyotoshi Sekiguchi, Tomohiro Shigemori
  • Publication number: 20190153391
    Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin ?2?1?1, laminin ?2?2?1, laminin ?1?1?1 and laminin ?1?2?1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.
    Type: Application
    Filed: September 14, 2015
    Publication date: May 23, 2019
    Applicant: OSAKA UNIVERSITY
    Inventors: Yukiko OCHI, Kiyotoshi SEKIGUCHI, Shigeru MIYAGAWA, Yoshiki SAWA, Antti Markus SILTANEN
  • Patent number: 10287541
    Abstract: Provided is a cell culture vessel characterized in that a surface to be in contact with cells is coated with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in a dry state, the laminin fragment being derived from at least one kind selected from laminin ?5?1?1 and laminin ?5?2?1, the cell culture vessel being any of the following: (1) a cell culture vessel of which a surface to be in contact with cells is coated only with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in a dry state; (2) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in combination with a laminin fragment having no integrin ?6?1 binding activity in a dry state; and (3) a cell culture vessel of which a surface to be in contact with cells is coated with a laminin fragment having integrin ?6?1 binding activity or a modified form thereof in combinatio
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: May 14, 2019
    Assignee: OSAKA UNIVERSITY
    Inventors: Kiyotoshi Sekiguchi, Ko Tsutsui
  • Publication number: 20190049429
    Abstract: A method for observing the dynamics of sweat glands and a method for evaluating a substance of interest, which are useful for development of a preparation for external application, such as a cosmetic. In each of the methods, an observation sample is used, which is prepared by staining all sweat glands, which are isolated alive, with a staining reagent, and then holding the all sweat glands on a support using at least one material selected from the group consisting of collagen, agarose, basement membrane matrix, poly-D-lysine and a membrane.
    Type: Application
    Filed: July 18, 2017
    Publication date: February 14, 2019
    Applicants: MANDOM CORPORATION, OSAKA UNIVERSITY
    Inventors: Kie Nakashima, Ryuichiro Kurata, Fumitaka Fujita, Kiyotoshi Sekiguchi, Atsushi Tanemura, Hiroyuki Murota, Ichiro Katayama
  • Publication number: 20180208893
    Abstract: Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.
    Type: Application
    Filed: July 14, 2016
    Publication date: July 26, 2018
    Inventors: Tatsutoshi Nakahata, Megumu Saito, Akira Niwa, Ryo Ota, Kiyotoshi Sekiguchi
  • Patent number: 10000554
    Abstract: A modified laminin characterized in that a laminin or a heterotrimeric laminin fragment has a collagen binding molecule conjugated to at least one site selected from the ? chain N-terminus, the ? chain N-terminus and the ? chain N-terminus, and an extracellular-matrix material comprising the modified laminin, and collagen and/or gelatin serve as an alternative to Matrigel and are useful as an extracellular-matrix material for the formation of a safe three-dimensional tissue structure for regenerative medicine in humans.
    Type: Grant
    Filed: November 11, 2013
    Date of Patent: June 19, 2018
    Assignee: OSAKA UNIVERSITY
    Inventors: Kiyotoshi Sekiguchi, Shaoliang Li, Ryoko Sato
  • Patent number: 9758765
    Abstract: Provided are a modified laminin having a cell-growth regulatory molecule bound to at least one site selected from the ? chain N-terminus, the ? chain C-terminus, the ? chain N-terminus and the ? chain N-terminus of laminin or a heterotrimeric laminin fragment, a method for culturing cells in the presence of the modified laminin, a method for establishing iPS cells in the presence of the modified laminin, and a culture substrate coated with the modified laminin. Human stem cells cultured in a xeno-free environment with the use of the modified laminin of the present invention can be provided as highly safe human stem cells applicable to regenerative medicine.
    Type: Grant
    Filed: April 9, 2012
    Date of Patent: September 12, 2017
    Assignees: OSAKA UNIVERSITY, KYOTO UNIVERSITY
    Inventors: Kiyotoshi Sekiguchi, Yukimasa Taniguchi, Masato Nakagawa