Abstract: The present invention relates to a mixing system comprising a vessel (1) for housing a disposable fluid mixing bag (2), which vessel (1) comprises a base (3) supporting the fluid mixing bag (2) and a sidewall (4) extending vertically upwards from the base (3). The sidewall (4) of the vessel comprises a door opening (5) which is closable by a sliding door (6). The system with the sliding door provides an easy access to the inside of the vessel (1) in a space saving manner.

Abstract: The invention discloses a system for perfusion culture of cells which comprises: a bioreactor; a filter unit with a retentate inlet end, a retentate outlet end and a permeate outlet port; a reciprocating pump fluidically connected to the retentate inlet end, with the retentate inlet end fluidically connected to the bioreactor via an inlet check valve arranged to allow flow in the direction from the bioreactor to the retentate inlet end and to block flow in the reverse direction, and where the retentate outlet end is fluidically connected to the bioreactor via an outlet check valve arranged to allow flow in the direction from the retentate outlet end to the bioreactor and to block flow in the reverse direction; and where the inlet and outlet check valves are each fluidically connected to a tubing branch point, which is further connected to the bioreactor via a length of tubing.

Abstract: Methods, systems, and apparatus to monitor component status in a bioprocessing environment are disclosed and described. Certain examples provide a sensor device for a disposable bioprocessing component. The example sensor device includes a first portion affixed to the component, the first portion configured to provide an identifier associated with the component. The example sensor device also includes a second portion configured to provide a status indication based on a state of the component. The example sensor device is configured to transmit the identifier and status indication to a control computer associated with a bioprocessing platform including the component.

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

Abstract: The invention relates to a system for packing chromatography columns with a chromatography medium and packing method for use in such columns. In particular, the invention relates to a method and system for packing chromatography columns where the column and chromatography media are pre-sterilized.

Abstract: A method for providing an aseptic chromatography column includes the steps of pre-sterilizing an empty chromatography column, pre-sterilizing a chromatography medium, introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thus providing an aseptic chromatography column comprising chromatography medium.

Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

Abstract: Methods, systems, and apparatus to monitor component status in a bioprocessing environment are disclosed and described. Certain examples provide a sensor device for a disposable bioprocessing component. The example sensor device includes a first portion affixed to the component, the first portion configured to provide an identifier associated with the component. The example sensor device also includes a second portion configured to provide a status indication based on a state of the component. The example sensor device is configured to transmit the identifier and status indication to a control computer associated with a bioproces sing platform including the component.

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

Abstract: The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use.

Abstract: The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use.

Abstract: The invention discloses a connector (1) for substantially aseptic connection of tubing, comprising: a) a central tubular stem member (2) with a line connection end (3) and a coupling end (5) having an annular gasket (6) arranged to engage a similar annular gasket (26) on a similar second connector (21) in sealing abutment and; b) a tubular socket member (7), concentrically arranged outside the stem member, having a flange (8) concentrically arranged outside the coupling end and comprising a cover film (9) releasably bonded to the flange and covering the coupling end of the stem member, the annular gasket and at least a portion of the flange; wherein the socket member is rotatable around the stem member and wherein the connector further comprises an annular seal member (10) in sealing abutment between an inside (11) of the socket member and an outside (12) of the stem member.

Abstract: The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.

Abstract: A chromatography column distribution system includes a set of first bed support ribs extending radially from an inner, first radial position near the centre of the plate to an outer radial position nearer to the periphery of the plate and at least one set of intermediate bed support ribs starting at an intermediate radial position and extending to an outer radial position nearer to the periphery of the plate. Channels are formed between adjacent bed support ribs. The desired local effective channel height varies in accordance with a predetermined formula from the first radial position to the outer radial position. The transverse cross-sectional areas of the ribs or the channels are adapted such that the actual local effective channel height is within 15% of the desired local effective channel height over portions of the distribution system situated between the first radial position and the outer radial position.

Abstract: A disposable container comprising a side wall, top and bottom, wherein the side wall comprises a first flexible material and the bottom comprises a rigid or semi-rigid material. The side wall top and bottom are joined together to define the container with an interior compartment for keeping a fluid inside the container. The bottom comprises at least one opening that is fluid-tightly covered with an assembly comprising a second flexible material. The assembly comprises at least one port which provides access to the interior compartment of the container. The invention also relates to a mixing system comprising the disposable container.

Abstract: An apparatus for substantially sterile connection of flexible tubing, including at least one tubing holder, which is adapted to receive one or more lengths of flexible tubing and which is also equipped with one or more sterilant applicators. The sterilant applicator is adapted to apply a sterilant to the length of tubing when the tubing length is received in the tubing holder. At least one cutter, which is movable in relation to the tubing holder and adapted to cut the length of tubing. A connector holder, which includes a compartment housing and a substantially sterile tubing connector. The connector holder is movable in relation to the tubing holder to a position where the length of tubing and the sterile tubing connector are aligned and can be urged together.

Abstract: A fluid distributor for uniformly distributing flow of a feed onto a chromatography column in a cost-effective way defines a distribution channel of generally conical shape between a solid back plate and a packed bed in the column, wherein the fluid distributor comprises at least two circular and/or annular fluid-permeable porous disks essentially filling the volume of the distribution channel.

Abstract: A chromatography column distribution system includes a set of first bed support ribs extending radially from an inner, first radial position near the centre of the plate to an outer radial position nearer to the periphery of the plate and at least one set of intermediate bed support ribs starting at an intermediate radial position and extending to an outer radial position nearer to the periphery of the plate. Channels are formed between adjacent bed support ribs. The desired local effective channel height varies in accordance with a predetermined formula from the first radial position to the outer radial position. The transverse cross-sectional areas of the ribs or the channels are adapted such that the actual local effective channel height is within 15% of the desired local effective channel height over portions of the distribution system situated between the first radial position and the outer radial position.

Abstract: A magnetic agitator mixing system comprising: a first magnetic coupling part, a first driving means provided with a second magnetic coupling part, and a container which is substantially enclosing the first magnetic coupling part. The first magnetic coupling part is rotatably suspended in a stand, which is arranged to rest on an inner surface of the container and a first impeller is arranged to be driven by the first magnetic coupling part. The invention also relates to an agitator mixing stand.