Patents by Inventor Koji Ushizawa

Koji Ushizawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method and reagent for quantitative analysis of 3-oxo-5.beta.-steroid
    Patent number: 5085989
    Abstract: A quantitative analysis of 3-oxo-5.beta.-steroid is carried out by acting 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical density of the chromophoric substance thereby produced. A typical reducing chromophoric agent is a tetrazolium compound. The invention also provides a reagent for the quantitative analysis of 3-oxo-5.beta.-steroid comprising 3-oxo-5.beta.-steroid.DELTA..sup.4 -dehydrogenase and a tetrazolium compound. The analysis and reagent provide a simple and reliable method for a liver function test.
    Type: Grant
    Filed: August 28, 1990
    Date of Patent: February 4, 1992
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Koji Ushizawa, Takae Shigihara, Maki Akemichi, Toshiyuki Akimoto, Senkichi Nagasaki, Miyoshi Hirata
  • 3.alpha.-hydroxysteroid oxidase and quantitative analysis of 3.alpha.-hydroxysteroid making use of same
    Patent number: 4889801
    Abstract: Disclosed herein is a 3.alpha.-hydroxysteroid oxidase which oxidizes a 3.alpha.-hydroxysteroid into a 3-oxosteroid and hydrogen peroxide. The oxidase may preferably be obtained from a culture broth of Pseudomonas testosteroni (ATCC 11996). The oxidase is useful for the quantitative analysis of a 3.alpha.-hydroxysteroid. Method and reagent useful for the quantitative analysis of a 3.alpha.-hydroxysteroid are also disclosed.
    Type: Grant
    Filed: June 19, 1987
    Date of Patent: December 26, 1989
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventor: Koji Ushizawa
  • Quantitative analysis of 3.alpha.-hydroxysteroid and reagent useful therefor
    Patent number: 4816394
    Abstract: A 3.alpha.-hydroxysteroid contained in a sample can be quantitatively analyzed by causing a 3.alpha.-hydroxysteroid dehydrogenase to act on the sample in the presence of nicotinamide adenine dinucleotide, causing tetrazolium or a salt thereof, an electron carrier substance, and 3-oxosteroid-.DELTA..sup.1 -dihydrogenase and/or 3-oxosteroid-.DELTA..sup.4 -dehydrogenase to act on the thus-obtained reaction product, and then quantitatively analyzing the resultant formazan.
    Type: Grant
    Filed: May 12, 1986
    Date of Patent: March 28, 1989
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventors: Koji Ushizawa, Akemichi Maki, Toshiyuki Akimoto, Senkichi Nagasaki, Miyoshi Hirata