Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.

Abstract: Provided herein is a bioprocessing device, bioprocessing card, and fluidics cartridge for performing automated bioprocessing of a sample. The bioprocessing card may include a plurality of pipette tips; and at least one pump in fluid communication with the plurality of pipette tips. In some embodiments, the pumps and the pipette tips are in fluid communication through a processing channel which may be a microscale channel. Also provided herein is an automated bioprocessing device comprising: at least one bioprocessing card; at least one fluidic cartridge; and an automated control system configured to control automated bioprocessing of a sample. Further provided herein are methods of use of the device, card, and cartridge.

Abstract: The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) having a magnetising portion (3) for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion (4) for retaining at least one sample vessel (5), wherein, the magnetising portion (3) is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (5); the sample vessel retaining portion (4) is configured to retain at least one sample vessel (5) such that at least a portion of the contents of the sample vessel (5) is visible to a user; and the sample vessel retaining portion (4) is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (3).

Abstract: The disclosure relates to a magnetic separation rack for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion (1) and a foot portion (8). The body portion comprises an array of sample vessel retaining portions (2) and plurality of magnetising portions (3). Each sample vessel retaining portion comprises at least one visible portion such that when a sample vessel is mounted in a sample vessel retaining portion at least one portion of the sample vessel is visible to a user. The magnetising portions are arranged within the body portion (1) such that at least two magnetising portions (3) are circumferentially spaced about each sample vessel retaining portion (2). The foot portion is pivotally coupled to the body portion such that the body portion is operatively tiltable with respect to the foot portion such that each sample vessel retaining portion may retain a sample vessel mounted therein in a tilted position with respect to the vertical.

Abstract: The present disclosure relates to a magnetising portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetising portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetising portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.

Abstract: One embodiment of the present invention provides a technique for detecting anomalies during operation of a test computer system. Initially, a golden system and the test system are equipped with the same hardware configuration, wherein the golden system has gone through extensive qualification testing and is presumed to be operating correctly. Next, a deterministic load is executed on the golden system, and values for performance parameters from the golden system are monitored while the deterministic load is executing. Similarly, the deterministic load is also executed on the test system, and values for performance parameters from the test system are monitored while the deterministic load is executing. Next, pairwise differences are computed between values for performance parameters received from the test system and values for performance parameters received from the golden system.