Abstract: Provided are a vector for multi-gene base editing, an editing method, and a use, and belongs to the technical field of gene editing. This application provides a vector for multi-gene base editing. In the vector, a replicon element, U6, and sgRNA are linked in series to allow a plasmid carrying the replicon element to persist in a cell, such that other elements on the plasmid can be continuously expressed in the cell, which improves the efficiency of multi-gene silencing. This application also provides a tandem vector, which is obtained by removing an epi element from the vector above. The two multi-gene editing vectors constructed in the embodiments of the present disclosure both have a silencing editing effect for target loci. The vector including the epi element has a higher editing efficiency than the vector without the epi element.

Abstract: Porous catalyst support and methods for producing same are provided herein. An example porous catalyst support includes a porous material defining a plurality of pores, wherein the porous material comprises a porous carbon material, the plurality of pores comprises a plurality of micropores and a plurality of mesopores, and a porosity of the porous catalyst has a predetermined volume of micropores and of mesopores.

Abstract: A battery includes a battery body having a body top surface, a body bottom surface, and a body side wall. The body side wall includes two oppositely disposed first side walls. Distance between outer surfaces of the two first side walls is L. First and second positions not coincide with each other are taken on midline of any first side wall, the first position is located in a range of 0 to 10 mm from the body top surface, the second position is located in a range of 0 to 10 mm from the body bottom surface, and distance between the first and second positions is H. Under conditions of ambient temperature being 25° C. and 2C current rate, temperature at first position is T1, temperature at second position is T2, and the battery satisfies (T1?T2)×3L/H?20° C.

Abstract: Provided is a method for detecting molecular characteristic of a gene-edited disease-resistant pigs and use thereof. A CRISPR/Cas12a nucleic acid detection system is provided, which includes a nucleic acid detection system 1; the nucleic acid detection system 1 includes crRNA1; and a nucleotide sequence of the crRNA1 is SEQ ID NO: 11. A method for detecting an AE26-CAAS gene-edited disease-resistant pig based on CRISPR/Cas12a system is provided, which has high sensitivity, strong specificity, is simple and easy to use, is convenient for rapid on-site detection, and is economical, simple, efficient and fast, thereby laying the foundation for the commercial production of a gene-edited disease-resistant pig.

Abstract: A negative electrode sheet and a battery applying the same are provided. The negative electrode sheet includes a current collector and a negative electrode active coating disposed on two opposite surfaces of the current collector. The negative electrode active coating contains a negative electrode active material, and the negative electrode active material includes graphite. The negative electrode sheet satisfies 0.0025??*?/PD?0.0065, where ? is a porosity of the negative electrode sheet, ? is a surface density of a single surface of the negative electrode sheet with a unit of g/cm2, and PD is a compacted density of the negative electrode sheet with a unit of g/cm3. The compacted density PD, the surface density ?, and the electrode sheet porosity ? of the negative electrode sheet form specific correlation relationships among one another.

Abstract: The present disclosure may provide an atomizer and an electronic atomization device. The atomizer may include a shell, a heating assembly, a lid, a base, and a first sealing component. The shell defines a liquid cavity and a smoke outlet. The heating assembly may heat and atomize the liquid into a smoke. The lid and the base may be provided to fix the heating assembly therebetween. The lid defines a liquid inlet communicating with the liquid cavity, and the liquid may flow through to reach the heating assembly. The atomizer may define an air entering hole, such that external air may enter the device through the hole to drive smoke to flow. The first sealing component may be engaged between the lid and the heating assembly, contacting both at the same time and defining air guiding channels to guide the air to flow into the liquid cavity.

Abstract: The present disclosure belongs to the field of medicines. A thyroid hormone ? receptor agonist, a crystalline form, a preparation method and the use thereof are provided. The compound is a compound represented by formula (I) or a pharmaceutically acceptable salt thereof. The X-ray powder diffraction pattern of a crystal form A of the compound of formula (I) comprises characteristic peaks at 6.10±0.20°, 12.08±0.20° and 16.49±0.20° 20, as determined by means of using Cu-K? radiation. The present invention develops a new 2-pyridone derivative. The compound has high agonistic activity and selectivity for the thyroid hormone ? receptor, and can be used for treating metabolic diseases, particularly for treating diseases related to thyroid hormone receptor. The present invention also studies a series of crystal forms of the compound. The crystal form A compound has the advantages of good stability, low hygroscopicity, and small heat influence.

Abstract: A pedal shaft (100) for a pedal-type power meter includes a hollow shaft (10) configured such that a pedal is rotatably attached to the hollow shaft (10), a strain gauge (SGX, SSG) attached to an outer peripheral surface of the hollow shaft, and a processing circuit (20) disposed at an interior space of the hollow shaft and configured to process an output from the strain gauge.

Abstract: A method for detecting bi-allelic gene edited cells based on CRISPR/Cas12a technology is provided. The method provides crRNA, a nucleotide sequence of which that binds target is SEQ ID NO: 6. The crRNA is transcribed from a transcription template in vitro; the transcription template is a product obtained by annealing a single stranded DNA molecule shown in SEQ ID NO: 10 and a single stranded DNA molecule shown in SEQ ID NO: 13. The method provides crRNA of CD71 gene and constructs a CRISPR/Cas12a system using the same for CRISPR/Cas12a detection, achieving the screening for CRISPR/Cas9 induced CD71 gene bi-allelic gene edited cells. This method is simple, easy, fast, and cost-effective for screening a large number of mutant cells.

Abstract: A medical input apparatus including an input module including a body portion, a first fixing portion, and a first electrical connection portion; and an operation panel including an accommodation portion for accommodating the body portion, a second fixing portion, and a second electrical connection portion, where the input module rotates relative to the operation panel along a first rotation path and is detachably connected to the operation panel, the first fixing portion is connected to the second fixing portion when the input module is located at a first position on the first rotation path, and the first electrical connection portion is connected to the second electrical connection portion when the input module is located at a second position on the first rotation path.

Abstract: A reagent, a kit, and a method for detecting a pig economic trait-associated mutation site are provided, belonging to the technical field of molecular markers. The reagent for detecting a pig economic trait-associated mutation site is provided, and the reagent and an endonuclease are prepared into the kit for detecting a pig economic trait-associated mutation site. The method for detecting a pig economic trait-associated mutation site is also provided. After PCR amplification, gene fragments of WIP1, TRIM55, and GAS7 of a pig show site differences between different individuals. Restriction endonucleases are used for digestion, and PCR or multiplex PCR analysis is conducted to identify single nucleotide polymorphisms (SNPs) of the pig, such that the wild type, the homozygous mutant type, and the heterozygous mutant type that are difficult to differentiate in morphology are differentiated from the molecular biology level.

Abstract: A state of charge (SOC) determination method and apparatus for a battery system are disclosed. Based on a correspondence between impedance and SOC, a correspondence between OCV and SOC, and an operating current at a previous data collection moment, a correction coefficient at a current data collection moment can be determined. After the SOC estimated at the current data collection moment is corrected based on the correction coefficient, the accumulation of errors in the SOC can be eliminated, and the accuracy of the determined SOC is thereby improved.

Abstract: The present disclosure may provide an atomizer and an electronic atomization device. The atomizer may include a shell, a heating assembly, a lid, a base, and a first sealing component. The shell defines a liquid cavity and a smoke outlet. The heating assembly may heat and atomize the liquid into a smoke. The lid and the base may be provided to fix the heating assembly therebetween. The lid defines a liquid inlet communicating with the liquid cavity, and the liquid may flow through to reach the heating assembly. The atomizer may define an air entering hole, such that external air may enter the device through the hole to drive smoke to flow. The first sealing component may be engaged between the lid and the heating assembly, contacting both at the same time and defining air guiding channels to guide the air to flow into the liquid cavity.

Abstract: A kit for breeding a pig breed with resistance to the porcine transmissible gastroenteritis virus infection and application thereof. The systems includes a genetically edited protein, pAPN-sgRNA-1, pAPN-sgRNA-2, and a donor DNA. Effectively enzymatic cleavage can be made in two target sites of a pAPN gene by the gene editing protein. By replacing the fragment to be site-directed modified located between two target sites with donor DNA, a codon encoding tryptophan at position 737 in pAPN protein can be mutated to a codon encoding alanine, thereby achieving precise mutation of tryptophan to alanine at position 737 in a pAPN protein. The systems can avoid disruption or alteration of the normal expression of other amino acids in pAPN protein, therefore, the present invention maximally retains the physiological activity function of pAPN protein on the basis of resisting TGEV infection.

Abstract: The present invention provides a pAPN mutant and a composition for site-directed modification of pAPN gene and application thereof, involving the field of gene editing technology. The mutated pAPN mutant with an alanine at position 734 can maintain its normal expression, while reducing the ability of a host expressing the pAPN mutant to specifically bind to TGEV, which has important scientific and practical significance in pig disease resistance breeding.

Abstract: The present application provides a key circuit, wherein the circuit includes: a plurality of keys, a plurality of driving lines, a plurality of induction lines and a control module, wherein the plurality of keys are divided into a plurality of regions according to parameters affecting force and resistance response, and resistance of the keys in each region changes with pressure within the same range; each key is connected to one driving line and one induction line, and keys in the same region are connected to the same induction line; and the plurality of driving lines and the plurality of induction lines are all connected to I/O interfaces of the control module, and are configured to transmit acquired resistance information of corresponding keys to the control module, so as to output control information corresponding to the keys via the control module.

Abstract: This application discloses a flexible printed circuit board and an electronic device. The flexible printed circuit board includes a body part, a coaxial cable, and a terminal disposed in the body part. The coaxial cable is disposed in the body part, and at least one end of the coaxial cable is electrically connected to outside by using the terminal.

Abstract: Embodiments of the disclosure provides a method, an apparatus, an electronic device and a storage medium for object processing. The method includes: in response to a deletion triggering operation, obtaining a target object to be deleted; determining object attribute data and a data association relationship corresponding to the target object, where the data association relationship is a reference relationship between the target object and a superior object containing the target object; obtaining serialized object data by serializing the object attribute data, and storing the serialized object data; and dissolving the data association relationship, and deleting the target object.

Abstract: Embodiments of the present disclosure provide a method, an apparatus, an electronic device, and a storage medium for data processing. The method comprises: in response to a copy trigger operation, obtaining an object to be copied, and determining a target component corresponding to the object to be copied; for each of the target component, obtaining a component object and an engine object of the target component, and determining target storage data corresponding to the target component based on the component object and the engine object, wherein the target storage data comprises a component object type field, an engine object type field, component object serialized data, and engine object serialized data; and storing the target storage data corresponding to the target component into a clipboard.

Abstract: This description relates to a substance causing a mutation at position 727 in an amino acid residue of the pAPN protein in any of the following: 1) constructing a cell line with site-directed modification of the pAPN gene for non-disease diagnostic and therapeutic purposes; 2) preparing a cell with resistance to the porcine transmissible gastroenteritis virus; and so on. A system for site-directed modification of the pAPN gene may effectively cleave two target sites of the pAPN gene, thereby achieving the precise mutation at position 727 in an amino acid of pAPN. Based on the precise modification of the pAPN gene while capable of avoiding disruption or alteration of the normal expression of other amino acids in the pAPN, the physiological activity function of pAPN protein on the basis of resisting TGEV infection is retained.