Abstract: This description relates to a substance causing a mutation at position 727 in an amino acid residue of the pAPN protein in any of the following: 1) constructing a cell line with site-directed modification of the pAPN gene for non-disease diagnostic and therapeutic purposes; 2) preparing a cell with resistance to the porcine transmissible gastroenteritis virus; and so on. A system for site-directed modification of the pAPN gene may effectively cleave two target sites of the pAPN gene, thereby achieving the precise mutation at position 727 in an amino acid of pAPN. Based on the precise modification of the pAPN gene while capable of avoiding disruption or alteration of the normal expression of other amino acids in the pAPN, the physiological activity function of pAPN protein on the basis of resisting TGEV infection is retained.

Abstract: The presently disclosed subject matter relates to devices, systems, and methods for fabricating a solid polymer electrolyte electrode assembly are provided. One or more electrode for a solid polymer electrolyte electrode assembly includes a porous substrate configured as a liquid/gas diffusion layer and an ionomer-free catalyst coated on the substrate.

Abstract: Provided is a fixing member used in a support structure (100) for a bed. The support structure includes a lower frame (10), an upper frame (20), a raising/lowering mechanism (30) configured to raise and lower the upper frame with respect to the lower frame and including a support shaft (312) extending in a width direction of the bed, and a supported member (221, 222) fixed to the upper frame and supported by the support shaft. The support shaft moves in a longitudinal direction of the bed while supporting the supported member when the upper frame is raised and lowered. The fixing member includes a plurality of fixing tools (S11 to S14) configured to fix the supported member to the upper frame. Each of the plurality of fixing tools includes a flexure element (SB) fixed to the upper frame at one end and fixed to the supported member at the other end, and a strain gauge (SG) attached to the flexure element.

Abstract: The present invention provides a pAPN mutant and a composition for site-directed modification of pAPN gene and application thereof, involving the field of gene editing technology. The mutated pAPN mutant with an alanine at position 734 can maintain its normal expression, while reducing the ability of a host expressing the pAPN mutant to specifically bind to TGEV, which has important scientific and practical significance in pig disease resistance breeding.

Abstract: Provided is a fixing member used in a support structure (100) for a bed. The support structure includes a lower frame (10), an upper frame (20), a raising/lowering mechanism (30) configured to raise and lower the upper frame with respect to the lower frame and including a support shaft (312) extending in a width direction of the bed, and a supported member (221, 222) fixed to the upper frame and supported by the support shaft. The support shaft moves in a longitudinal direction of the bed while supporting the supported member when the upper frame is raised and lowered. The fixing member includes a plurality of fixing tools (S11 to S14) configured to fix the supported member to the upper frame. Each of the plurality of fixing tools includes a flexure element (SB) fixed to the upper frame at one end and fixed to the supported member at the other end, and a strain gauge (SG) attached to the flexure element.

Abstract: A kit for breeding a pig breed with resistance to the porcine transmissible gastroenteritis virus infection and application thereof. The systems includes a genetically edited protein, pAPN-sgRNA-1, pAPN-sgRNA-2, and a donor DNA. Effectively enzymatic cleavage can be made in two target sites of a pAPN gene by the gene editing protein. By replacing the fragment to be site-directed modified located between two target sites with donor DNA, a codon encoding tryptophan at position 737 in pAPN protein can be mutated to a codon encoding alanine, thereby achieving precise mutation of tryptophan to alanine at position 737 in a pAPN protein. The systems can avoid disruption or alteration of the normal expression of other amino acids in pAPN protein, therefore, the present invention maximally retains the physiological activity function of pAPN protein on the basis of resisting TGEV infection.

Abstract: A screwing machine includes a motor, an output shaft, a twist portion, a plate portion, a strain gauge, an amplification circuit. The output shaft is driven by the motor. To the output shaft, a bit is attachable. The twist portion is hollow. To the twist portion, torque applied to the output shaft is transmitted through a transmission element. The output shaft is configured to be indirectly twisted by the torque applied to the output shaft. The plate portion is integrally formed with the twist portion. The strain gauge is disposed on the twist portion. The amplification circuit is disposed on the plate portion. To the amplification circuit, a signal from the strain gauge is input.

Abstract: An atomizer may include a housing and an atomizing assembly. The housing may include a nozzle and a liquid storage pipe. The nozzle may be located at an end of the liquid storage pipe and integrated with the liquid storage pipe. At least a portion of an outer surface of the nozzle away from the liquid storage pipe may include a matte surface having a first rugosity. At least a portion of an outer surface of the housing may be a polished surface through which aerosol-generating substrate stored in the liquid storage pipe can be observed. The atomizing assembly may be configured to heat and atomize the aerosol-generating substrate to generate smoke.

Abstract: An over-energy protection circuit, a residual current device, an electronic device, and a power distribution box. The protection circuit has relatively high operating reliability, and includes: a first over-energy absorption circuit, a second over-energy absorption circuit, and a residual current determining circuit. The first over-energy absorption circuit receives a first electrical signal output from a current transformer; and when a voltage value of the first electrical signal is greater than or equal to a first preset threshold, performs step-down processing on the first electrical signal to obtain a second electrical signal, and outputs the second electrical signal to the second over-energy absorption circuit; or when a voltage value of the first electrical signal is less than a first preset threshold, outputs the first electrical signal to the second over-energy absorption circuit.

Abstract: The present disclosure may provide an atomizer and an electronic atomization device. The atomizer may include a shell, a heating assembly, a lid, a base, and a first sealing component. The shell defines a liquid cavity and a smoke outlet. The heating assembly may heat and atomize the liquid into a smoke. The lid and the base may be provided to fix the heating assembly therebetween. The lid defines a liquid inlet communicating with the liquid cavity, and the liquid may flow through to reach the heating assembly. The atomizer may define an air entering hole, such that external air may enter the device through the hole to drive smoke to flow. The first sealing component may be engaged between the lid and the heating assembly, contacting both at the same time and defining air guiding channels to guide the air to flow into the liquid cavity.

Abstract: An atomizer may include a housing and an atomizing assembly. The housing may include a nozzle and a liquid storage pipe. The nozzle may be located at an end of the liquid storage pipe and integrated with the liquid storage pipe. At least a portion of an outer surface of the nozzle away from the liquid storage pipe may include a matte surface having a first rugosity. At least a portion of an outer surface of the housing may be a polished surface through which aerosol-generating substrate stored in the liquid storage pipe can be observed. The atomizing assembly may be configured to heat and atomize the aerosol-generating substrate to generate smoke.

Abstract: The present disclosure may provide an atomizer and an electronic atomization device. The atomizer may include a shell, a heating assembly, a lid, a base, and a first sealing component. The shell defines a liquid cavity and a smoke outlet. The heating assembly may heat and atomize the liquid into a smoke. The lid and the base may be provided to fix the heating assembly therebetween. The lid defines a liquid inlet communicating with the liquid cavity, and the liquid may flow through to reach the heating assembly. The atomizer may define an air entering hole, such that external air may enter the device through the hole to drive smoke to flow. The first sealing component may be engaged between the lid and the heating assembly, contacting both at the same time and defining air guiding channels to guide the air to flow into the liquid cavity.

Abstract: The present invention provides a polysubstituted nitrogen-containing heterocyclic derivative, a preparation method, and use thereof. In the present invention, the novel structure of the natural nitrogen-containing heterocyclic molecule is designed to obtain the polysubstituted nitrogen-containing heterocyclic derivative. The polysubstituted nitrogen-containing heterocyclic derivatives have obvious bactericidal activity, and some compounds have significant bactericidal activity against diseases such as E. carotovora, E. coli, and R. solanacearum, which can be widely applied in the comprehensive prevention and control of agricultural and forestry bacterial diseases. The bactericide obtained by mixing the polysubstituted nitrogen-containing heterocyclic derivative of the present invention with various adjuvants also have good bactericidal effects on E. coli, E. carotovora, R. solanacearum, etc.

Abstract: A negative electrode sheet and a battery applying the same are provided. The negative electrode sheet includes a current collector and a negative electrode active coating disposed on two opposite surfaces of the current collector. The negative electrode active coating contains a negative electrode active material, and the negative electrode active material includes graphite. The negative electrode sheet satisfies 0.0025??*?/PD?0.0065, where ? is a porosity of the negative electrode sheet, ? is a surface density of a single surface of the negative electrode sheet with a unit of g/cm2, and PD is a compacted density of the negative electrode sheet with a unit of g/cm3. The compacted density PD, the surface density ?, and the electrode sheet porosity ? of the negative electrode sheet form specific correlation relationships among one another.

Abstract: The disclosure discloses a battery device, a detection method thereof, a method and device for screening battery cells. The characteristic values are determined according to parameters of two peaks in the frequency-domain impedance diagram. The characteristic values may reflect the magnitude of charge transfer impedance and diffusion impedance in each battery cell, then reflect the characteristics of the interface and characteristics of solid phase particles during charging and discharging, and further reflect the health and performance of battery cells. The battery set screened in this way still have good consistency after storage and shelving, and the consistency between battery cells will not deteriorate after storage for a period of time, so that the battery device may have better performance.

Abstract: The invention provides a lithium-ion battery electrode piece and a lithium-ion battery including the same. The electrode piece includes an electrode active material layer disposed on the surface of the current collector and a protective layer disposed on the surface of the electrode active material layer. The protective layer is composed of metal oxide layers and conductive layers alternately stacked in the horizontal direction. The area ratio of the metal oxide layers to the conductive layers is 4:1-1:4, and the thickness ratio is 0.5:1-1:1. According to the invention, by regulating the shape and ratio of the high temperature stability material and the conductive active material on the electrode piece in the process of coating the protective layer, the amount of the two coating materials is precisely controlled to implement the precise regulation of the high temperature stability and rate performance of the electrode piece.

Abstract: A downhole throttling device based on wireless control includes an inlet nozzle, a throttling assembly, an electrical sealing cylinder, a gas guide cylinder, a lower adapter sleeve, an end socket, a female sleeve, and electrical components. The inlet nozzle is connected to the throttling assembly, the throttling assembly is connected to the electrical sealing cylinder and the gas guide cylinder, the electrical sealing cylinder and the gas guide cylinder are both connected to the lower adapter sleeve, the lower adapter sleeve is respectively connected to the end socket and the female sleeve, and the electrical components are arranged in the electrical sealing cylinder. A throttling effect is achieved by detecting the temperature and pressure in a tube by a temperature/pressure sensor in the electrical components and controlling a motor to rotate a movable valve in the throttling assembly by a circuit control assembly, thereby achieving wireless control over downhole throttling.

Abstract: A battery includes a battery body having a body top surface, a body bottom surface, and a body side wall. The body side wall includes two oppositely disposed first side walls. Distance between outer surfaces of the two first side walls is L. First and second positions not coincide with each other are taken on midline of any first side wall, the first position is located in a range of 0 to 10 mm from the body top surface, the second position is located in a range of 0 to 10 mm from the body bottom surface, and distance between the first and second positions is H. Under conditions of ambient temperature being 25° C. and 2C current rate, temperature at first position is T1, temperature at second position is T2, and the battery satisfies (T1?T2)×3L/H?20° C.

Abstract: A state of charge (SOC) determination method and apparatus for a battery system are disclosed. Based on a correspondence between impedance and SOC, a correspondence between OCV and SOC, and an operating current at a previous data collection moment, a correction coefficient at a current data collection moment can be determined. After the SOC estimated at the current data collection moment is corrected based on the correction coefficient, the accumulation of errors in the SOC can be eliminated, and the accuracy of the determined SOC is thereby improved.

Abstract: The invention discloses an electrolyte solution containing lithium salt, an organic solvent, and additives. The additives include vinylene carbonate and 2,6-di-tert-butyl-4-methylphenol; the content of vinylene carbonate accounts for 0.5% to 5% of the total mass of the electrolyte, and the content of 2,6-di-tert-butyl-4-methylphenol accounts for 7*10?4% to 1.6% of the total mass of vinylene carbonate. The electrolyte of the invention can improve the film formation rate and film thickness of vinylene carbonate in the negative electrode, so that the obtained SEI film resistance is smaller, thereby improving the performance of the lithium battery.