Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract: Provided herein are cartridges, devices, and methods for carrying out multistep assays on a microfluidic scale. A cartridge includes a block frame comprising a well, wherein the well comprises an outlet, at least one inlet, and a bottom surface; a plurality of containers, wherein each container is connected to the well via a microchannel leading to the at least one inlet; and an openable cover, which cover when closed is configured to enclose an assay surface and form a gap between (i) the assay surface and the bottom surface of the well or (ii) the assay surface and the cover. The gap can be formed by a spacer that extends from the bottom surface of the well or from the outer edge of the cover. Methods include flowing liquid into the gap and/or through an opening adjacent to the assay surface.

Abstract: Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate.

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract: Cells in a suspension are counted in a hemocytometer slice with a chamber of controlled depth and one or more reservoirs along one or more side edges of the chamber. The suspension is fed to a reservoir to first fill the reservoir, and then to overflow into the chamber. The result is an even distribution of the cells in the chamber.

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

Abstract: Rapid and uniform temperature changes in the wells of a microplate or any thin-walled plate that contains an array of reaction wells or sample receptacles are achieved by the use of heating and cooling elements with a vapor chamber interposed between such elements and the microplate. The upper surface of the vapor chamber and the underside of the sample plate in certain embodiments are complementary in shape, i.e., they have identical but oppositely directed contours in the areas around each of the sample receptacles, to provide continuous surface contact along the surface of each receptacle. In other embodiments, an intermediary plate is placed between the vapor chamber and the well plate, with the top surface of the intermediary plate being complementary in shape to the underside of the well plate.

Abstract: The present invention relates to human Akt3 proteins and polypeptides. The invention also relates to isolated nucleic acids encoding human Akt3, to vectors containing them and to their therapeutic uses, in particular for gene therapy. Expression of Akt3 inhibits cell death associated with hypoxia, apoptosis or necrosis.

Abstract: Cells in a suspension are counted in a hemocytometer slice with a chamber of controlled depth and one or more reservoirs along one or more side edges of the chamber. The suspension is fed to a reservoir to first fill the reservoir, and then to overflow into the chamber. The result is an even distribution of the cells in the chamber.

Abstract: An electronic device includes a shell defining a space and an opening therein. A fixing pole extends into the space from an inner surface of the shell adjacent to the opening. A circuit board is received in the space, and defines a through hole corresponding to the fixing pole. A push button is arranged in the shell, and includes a button body extending into the opening of the shell and being exposed to the outside, a fixing member, and a connecting member interconnecting the fixing member and the button body. The fixing member defines a fixing hole receiving the fixing pole therein. Two opposite sides of the fixing member abut against an inner surface of the shell and the circuit board, respectively. A locking member extends through the through hole and the fixing hole into the fixing pole to assemble the circuit board, the push button to the shell.

Abstract: An electronic device includes a main body and a lid body. The main body includes a fixing portion and a rotating component received in the fixing portion. The lid body has a guide arm accommodating the fixing portion and engaging with the fixing portion via the rotating component.

Abstract: A portable apparatus includes an electronic device defining a locking slot and a positioning device for detachably attached to the electronic device. The positioning device includes a cover defining an opening and a through hole; a supporting member comprising a hook protruding from the through hole to insert in the locking slot to lock in the electronic device and a supporting portion protruding from the opening of the cover to support the electronic device; and a dampening member fixed to the supporting member in the cover.

Abstract: An exemplary electronic apparatus (100) includes a main body (10), a lid (20) connecting with and rotating with respect to the main body between an opened position and a closed position, and a sliding bar (35) located in the main body. The main body defines a fastening hole (137), and includes a block portion (132) extending upwardly from an outer edge of the fastening hole. The block portion includes a side block portion (131) and a top block portion (134) connecting with the side block portion. The lid includes a hook (31) aligning with the fastening hole. The sliding bar includes a main bar (351) movably fastened onto the main body, a limiting portion (353) extending from the main bar, and a hook portion (357) extending from the limiting portion for fastening onto the hook. The limiting portion is blocked between the side block portion and the top block portion.

Abstract: An electronic device includes a housing defining a through hole, a circuit board fixed in the housing, including a switch and a grounding portion connecting with the ground, a fastening member received in the through hole fixed on the housing and contacting the grounding portion, passing static electricity therethrough and defining a mounting hole, and an actuating member received in the mounting hole and contacting the fastening member, and pressing the switch when actuated; wherein the fastening member and the actuating member are conductive.

Abstract: The present invention relates to human Akt3 proteins and polypeptides. The invention also relates to isolated nucleic acids encoding human Akt3, to vectors containing them and to their therapeutic uses, in particular for gene therapy. Expression of Akt3 inhibits cell death associated with hypoxia, apoptosis or necrosis.

Abstract: A hinge assembly and a portable electronic device are provided. The portable electronic device includes a lid, a main body, and a hinge assembly. The hinge assembly pivotly connects the lid to the main body. The hinge assembly includes a pivot element, a torsion spring, and a hinge brace. The pivot element is formed on the lid and partially and rotatably received in the main body. The torsion spring is assembled between the lid and the main body. When the lid is in a closed position with respect to the main body, the torsion spring becomes constricted and accumulates potential energy. When the lid is opened from the main body, the potential energy of the torsion spring is released to drive the lid to rotate to an open position with respect to the main body. The hinge brace is attached to the main body for blocking an excessive rotation of the lid with respect to the main body.

Abstract: An electronic apparatus (10) includes an enclosure (30), an antenna (20) and a locking mechanism (40). The antenna has a mount end (24) and an opposite free end (22). The mount end is pivotably mounted to the enclosure. The free end defines an engaging groove (220) therein. The locking mechanism includes a hook (50) for being engaged in the engaging groove of the antenna, a resilient mount (60) connected with the hook and capable of rotating with the hook, a supporting unit (80) for mounting the resilient mount to the enclosure, and a elastic member (70) for providing a resilient force when the hook and the resilient mount rotates.