Patents by Inventor Kyle Gee

Kyle Gee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Composition and method for measuring thallium influx and efflux
    Patent number: 9476892
    Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: October 25, 2016
    Assignee: Life Technologies Corporation
    Inventors: Daniel Beacham, Kyle Gee
  • Fluorescent Metal Ion Indicators with Large Stokes Shifts
    Publication number: 20160305964
    Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
    Type: Application
    Filed: May 11, 2016
    Publication date: October 20, 2016
    Inventors: Kyle GEE, Vladimir MARTIN
  • SUBSTRATES AND METHODS FOR STAINING LIVE STEM CELLS
    Publication number: 20160290994
    Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.
    Type: Application
    Filed: April 4, 2016
    Publication date: October 6, 2016
    Inventors: Uma LAKSHMIPATHY, Upinder SINGH, Scott GRECIAN, Rene QUINTANILLA, Kyle GEE, Mahendra RAO
  • Alkynyl-Derivatized Cap Analogs, Preparation and Uses Thereof
    Publication number: 20160281095
    Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
    Type: Application
    Filed: June 10, 2016
    Publication date: September 29, 2016
    Inventors: Anilkumar KORE, Shanmugasundaram MUTHIAN, Kyle GEE
  • SDP-containing heterobifunctional agents
    Patent number: 9440925
    Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: September 13, 2016
    Assignee: Life Technologies Corporation
    Inventors: Kyle Gee, Aimei Chen, Hee Chol Kang
  • 7-hydroxycoumarin-based cell-tracking reagents
    Patent number: 9400273
    Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.
    Type: Grant
    Filed: July 16, 2015
    Date of Patent: July 26, 2016
    Assignee: Life Technologies Corporation
    Inventors: Jixiang Liu, Hee Chol Kang, Kyle Gee, Christopher Langsdorf, Jolene Bradford, Gayle Buller
  • Alkynyl-derivatized cap analogs, preparation and uses thereof
    Patent number: 9388420
    Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: July 12, 2016
    Assignee: Life Technologies Corporation
    Inventors: Anilkumar Kore, Shanmugasundaram Muthian, Kyle Gee
  • Fluorogenic hydrazine-substituted compounds
    Patent number: 9389183
    Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: July 12, 2016
    Assignee: Life Technologies Corporation
    Inventors: Aimei Chen, Kyle Gee, Hee Chol Kang
  • Fluorescent metal ion indicators with large stokes shifts
    Patent number: 9372181
    Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
    Type: Grant
    Filed: June 17, 2014
    Date of Patent: June 21, 2016
    Assignee: Life Technologies Corporation
    Inventors: Kyle Gee, Vladimir Martin
  • FLUOROGENIC PH SENSITIVE DYES AND THEIR METHOD OF USE
    Publication number: 20160139158
    Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
    Type: Application
    Filed: November 10, 2015
    Publication date: May 19, 2016
    Inventors: Jeffrey DZUBAY, Kyle GEE, Vladimir MARTIN, Aleksey RUKAVISHNIKOV, Daniel BEACHAM
  • Optically-Detectable Enzyme Substrates and Their Method of Use
    Publication number: 20160139135
    Abstract: The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a ?-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a ?-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a ?-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a ?-lactmase or instructions for using the components in an assay.
    Type: Application
    Filed: September 11, 2014
    Publication date: May 19, 2016
    Inventors: Schuyler CORRY, William Downey, Brian Filanoski, Kyle Gee, Lawrence Greenfield, James Hirsch, Iain Johnson, Aleksey Rukavishnikov
  • Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol
    Patent number: 9341631
    Abstract: Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.
    Type: Grant
    Filed: June 11, 2014
    Date of Patent: May 17, 2016
    Assignee: Life Technologies Corporation
    Inventors: Kyle Gee, Vladimir Martin
  • Substrates and methods for staining live stem cells
    Patent number: 9334523
    Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.
    Type: Grant
    Filed: February 14, 2012
    Date of Patent: May 10, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Uma Lakshmipathy, Upinder Singh, Scott Grecian, Rene Quintanilla, Kyle Gee, Mahendra Rao
  • SDP-Containing Heterobifunctional Agents
    Publication number: 20160031821
    Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.
    Type: Application
    Filed: July 28, 2015
    Publication date: February 4, 2016
    Inventors: Kyle GEE, Aimei CHEN, Hee Chol KANG
  • SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS
    Publication number: 20160025713
    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.
    Type: Application
    Filed: October 6, 2015
    Publication date: January 28, 2016
    Inventors: Kyle Gee, Courtenay Hart-Kerndt, Wai-Yee Leung, Wayne Patton, Aleksey Rukavishnikov, Richard Haugland, Zhenjun Diwu
  • DETECTION OF RNA WITH SUBSTITUTED UNSYMMETRICAL CYANINE DYES
    Publication number: 20160010145
    Abstract: Substituted unsymmetrical cyanine dyes are provided herein, including kits, assays systems and methods for their use in the detection of RNA compounds (e.g., miRNA).
    Type: Application
    Filed: December 6, 2013
    Publication date: January 14, 2016
    Inventors: Jason DALLWIG, Kyle GEE, Nancy AHNERT, Hee Chol KANG, Jongtae YANG
  • Fluorinated resorufin compounds and their application
    Patent number: 9212385
    Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.
    Type: Grant
    Filed: September 12, 2012
    Date of Patent: December 15, 2015
    Assignee: Life Technologies Corporation
    Inventors: Robert H. Batchelor, Yue Ge, Iain Johnson, Wai-Yee Leung, Jixiang Liu, Brian Patch, Peter Smalley, Thomas Steinberg, Kyle Gee
  • Labeling Reagents and Methods of Their Use
    Publication number: 20150355186
    Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
    Type: Application
    Filed: August 18, 2015
    Publication date: December 10, 2015
    Inventors: Evan ANTOULINAKIS, Kyle GEE, Aleksey RUKAVISHNIKOV
  • Site-specific labeling of affinity tags in fusion proteins
    Patent number: 9164099
    Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6.
    Type: Grant
    Filed: May 8, 2013
    Date of Patent: October 20, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Gee, Aleksey Rukavishnikov, Courtenay Hart Kerndt, Richard Haugland, Wai-Yee Leung, Wayne Patton, Zhenjun Diwu
  • SDP-containing heterobifunctional agents
    Patent number: 9145361
    Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: September 29, 2015
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Gee, Aimei Chen, Hee Chol Kang