Patents by Inventor Kyle Gee

Kyle Gee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Labeling reagents and methods of their use
    Patent number: 9140706
    Abstract: The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: September 22, 2015
    Assignee: Life Technologies Corporation
    Inventors: Evan Antoulinakis, Kyle Gee, Aleksey Rukavishnikov
  • Alkynyl-Derivatized Cap Analogs, Preparation and Uses Thereof
    Publication number: 20150252373
    Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
    Type: Application
    Filed: January 20, 2015
    Publication date: September 10, 2015
    Inventors: Anilkumar KORE, Shanmugasundaram Muthian, Kyle Gee
  • DERIVATIVES OF 1,2-DIHYDRO-7-HYDROXYQUINOLINES CONTAINING FUSED RINGS
    Publication number: 20150247863
    Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
    Type: Application
    Filed: May 12, 2015
    Publication date: September 3, 2015
    Inventors: Zhenjun DIWU, Jixiang LIU, Kyle GEE
  • METHODS AND COMPOSITIONS FOR ENZYME-MEDIATED SITE-SPECIFIC RADIOLABELING OF GLYCOPROTEINS
    Publication number: 20150246146
    Abstract: Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.
    Type: Application
    Filed: October 25, 2013
    Publication date: September 3, 2015
    Inventors: Brian Agnew, Robert Aggeler, Hee Chol Kang, Aimei Chen, Kyle Gee
  • Fluorogenic pH-Sensitive Dyes and Their Methods of Use
    Publication number: 20150241438
    Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
    Type: Application
    Filed: March 14, 2013
    Publication date: August 27, 2015
    Inventors: Kyle Gee, Upinder Singh, Aleksey Rukavishnikov, Daniel Beacham, Shih-Jung Huang, Michael Janes, Wenjun Zhou, Judith Berlier
  • Use of Novel Coumarins as Glutathione and Thiol Labels
    Publication number: 20150219634
    Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.
    Type: Application
    Filed: February 6, 2015
    Publication date: August 6, 2015
    Inventors: Kyle GEE, Michael Janes, Iain Johnson, Hee Chol Kang
  • Fluorogenic pH-Sensitive Dyes and Their Methods of Use (II)
    Publication number: 20150218379
    Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
    Type: Application
    Filed: March 14, 2013
    Publication date: August 6, 2015
    Inventors: Kyle Gee, Upinder Singh, Aleksey Rukavishnikov, Daniel Beacham, Shih-Jung Huang, Michael Janes, Wenjun Zhou
  • Integrated lavatory galley monument
    Patent number: 9079668
    Abstract: A monument assembly configured to be positioned interior of an aircraft that includes a galley module, a first lavatory module and a second lavatory module. The module is positioned between the first and second lavatory modules, and the first lavatory module and the galley module share a first inboard dividing wall that includes at least a first non-vertical wall section.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 14, 2015
    Assignee: C&D Zodiac, Inc.
    Inventors: Kyle Gee, Marisa Ouchi, Nicholas Lee, Scott Savian
  • COMPOSITION AND METHOD FOR MEASURING THALLIUM INFLUX AND EFFLUX
    Publication number: 20150104823
    Abstract: The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. The loading buffer comprises at least one thallium indicator (e.g., an environmentally sensitive, luminescent dye) and a physiological concentration of chloride ions. The methods further comprise providing a stimulus buffer to the cell, wherein the stimulus buffer comprises thallium (e.g., thallium ions). Providing the stimulus buffer causes thallium influx into the cell through the ion channel. After providing the stimulus buffer, the luminescence (e.g., fluorescence) of the dye in the cell is detected. The luminescence of the dye can change in the presence or absence of thallium. The methods may be used to measure influx or efflux of thallium through an ion channel.
    Type: Application
    Filed: October 22, 2014
    Publication date: April 16, 2015
    Inventors: Daniel BEACHAM, Kyle GEE
  • Modified Nucleic Acid Binding Cyanine Dyes for the Detection of Reactive Oxygen Species
    Publication number: 20150079622
    Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.
    Type: Application
    Filed: February 26, 2013
    Publication date: March 19, 2015
    Inventors: Yi-Zhen Hu, Aimei Chen, Hee Chol Kang, Kyle Gee, Bhaskar Mandavilli
  • Use of novel coumarins as glutathione and thiol labels
    Patent number: 8981100
    Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: March 17, 2015
    Assignee: Life Technologies Corporation
    Inventors: Hee Chol Kang, Kyle Gee, Iain Johnson, Michael Janes
  • Compounds and Methods for Conjugation of Biomolecules
    Publication number: 20150072396
    Abstract: Low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and Staudinger ligations are also provided.
    Type: Application
    Filed: March 1, 2012
    Publication date: March 12, 2015
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle Gee, Upinder Singh, Scott Grecian, Scott Clarke
  • Benzoxazole-based fluorescent metal ion indicators
    Patent number: 8968997
    Abstract: Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.
    Type: Grant
    Filed: August 13, 2013
    Date of Patent: March 3, 2015
    Assignee: Life Technologies Corporation
    Inventors: Kyle Gee, Vladimir Martin
  • Alkynyl-derivatized cap analogs, preparation and uses thereof
    Patent number: 8969545
    Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.
    Type: Grant
    Filed: October 18, 2012
    Date of Patent: March 3, 2015
    Assignee: Life Technologies Corporation
    Inventors: Anilkumar R. Kore, Shanmugasundaram Muthian, Kyle Gee
  • LABELING AND DETECTION OF POST TRANSLATIONALLY MODIFIED PROTEINS
    Publication number: 20150051096
    Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.
    Type: Application
    Filed: July 14, 2014
    Publication date: February 19, 2015
    Inventors: Brian AGNEW, Kyle GEE, Tamara NYBERG
  • FLUOROGENIC PH SENSITIVE DYES AND THEIR METHOD OF USE
    Publication number: 20150037834
    Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
    Type: Application
    Filed: August 8, 2014
    Publication date: February 5, 2015
    Inventors: Daniel BEACHAM, Jeffrey DZUBAY, Kyle GEE, Vladimir MARTIN, Aleksey RUKAVISHNIKOV
  • OLIGOSACCHARIDE MODIFICATION AND LABELING OF PROTEINS
    Publication number: 20140377837
    Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
    Type: Application
    Filed: June 9, 2014
    Publication date: December 25, 2014
    Inventors: Brian AGNEW, Kyle Gee, Schuyler Corry
  • Fluorescent Metal Ion Indicators with Large Stokes Shifts
    Publication number: 20140363824
    Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
    Type: Application
    Filed: June 17, 2014
    Publication date: December 11, 2014
    Inventors: Kyle Gee, Vladimir Martin
  • LONG WAVELENGTH FLUOROGENIC INTRACELLULAR ION INDICATORS THAT ARE WELL RETAINED IN THE CYTOSOL
    Publication number: 20140363839
    Abstract: Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (Mc), a reporter molecule and two or more lipophilic groups (GL) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.
    Type: Application
    Filed: June 11, 2014
    Publication date: December 11, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kyle GEE, Vladimir MARTIN
  • Derivatives of 1,2-dihydro-7-hydroxyquinolines Containing Fused Rings
    Publication number: 20140356873
    Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
    Type: Application
    Filed: August 18, 2014
    Publication date: December 4, 2014
    Inventors: Zhenjun DIWU, Jixiang LIU, Kyle GEE