Abstract: Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic ion exchange material such that the sample reacts with the inorganic ion exchange material, and contacting the reacted sample with a means for capturing microorganisms.

Abstract: Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic on exchange material such that the sample reacts with the inorganic on exchange material, and contacting the reacted sample with a means for capturing microorganisms.

Abstract: Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

Abstract: Methods and compositions for enhancing reaction efficiency and sensitivity in polymerase chain reaction (PCR) are disclosed.

Abstract: Provided is a method of separating cells using a hydrophobic solid support. The method comprises contacting a solution containing cells with a hydrophobic solid support having a water contact angle between 70 and 90 degrees. By allowing cells to be adsorbed to the hydrophobic solid substrate, the cells can be separated. Thus, the cell separation efficiency can be rapidly and simply increased.

Abstract: A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.

Abstract: A microfluidic device includes; an ion exchange membrane, an anode chamber one side of which contacts a surface of the ion exchange membrane, wherein the anode chamber further includes a ladder-shaped anode and an anode support part, and a cathode chamber, one side of which contacts a surface of the ion exchange membrane opposite the anode chamber, wherein the cathode chamber further comprises a cathode, wherein the ladder-shaped anode is formed on a first surface of the anode support part, openings are formed in the anode support part which conform to the shape of the ladder-shaped anode, and a second surface of the anode support part which opposes the first surface of the anode contacts and supports the ion exchange membrane.

Abstract: Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

Abstract: Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.

Abstract: A method and apparatus for efficiently isolating nucleic acids from a nucleic acid-containing sample.

Abstract: Provided is a method of controlling the pH of a solution using electrolysis in a microfluidic device comprising an electrolysis device including an anode chamber, a cathode chamber, and a partition membrane between the anode chamber and the cathode chamber, wherein the anode chamber includes an inlet and an outlet through which an anode chamber solution enters and is discharged from the anode chamber, respectively, and an electrode, and the cathode chamber includes an inlet and an outlet through which a cathode chamber solution enters and is discharged from the cathode chamber, respectively, and an electrode.

Abstract: Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

Abstract: Provided are a method of isolating a nucleic acid from a sample and a solid material for isolating the nucleic acid which can be used for the above method. The method includes contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first pH to bind the nucleic acid to the bifunctional material, the bifunctional material being positively charged at the first pH; and releasing the nucleic acid at a second pH which is higher than the first pH.

Abstract: Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

Abstract: A microfluidic valve unit for controlling the flow of fluid flowing along a channel, and a method of fabricating the same are provided. The valve unit includes a channel formed in a platform; a valve filler which includes a phase transition material that is disposed in the channel in a solid state at room temperature to close the channel and is melted when energy is applied thereto; and at least one capillary channel which extends from a sidewall of the channel and into which the valve filler flows when the valve filler is melted, wherein the at least one capillary channel has a cross-sectional area which is less than a cross-sectional area of the channel.

Abstract: Provided are a method of separating genome DNA and plasmid DNA from each other from a sample using a binding buffer containing a high concentration kosmotropic salt and chaotropic salt, and a kit therefor.

Abstract: Provided are a microfluidic structure including a polysiloxane layer and a method of fabricating the microfluidic structure. The polysiloxane layer is coupled to substrates via a SiO2 layer.

Abstract: A method of manufacturing a DNA (deoxyribonucleic acid) chip is provided. The DNA chip has a plurality of transistors formed on a substrate and an organic layer and a DNA probe sequentially stacked on a gate of the transistor. The method includes forming an inter-layer insulation layer on the substrate to cover the transistors, planarizing the inter-layer insulation layer, forming at least two contact holes exposing gate electrodes of the transistors in the inter-layer insulation layer, selectively forming organic layers on the exposed gate electrodes, attaching a first DFR (dry film resist) layer to the upper surface of the inter-layer insulation layer to cover the contact holes, removing a portion of the first DFR layer covering a first contact hole among the contact holes, attaching a first DNA probe to the organic layers in the first contact hole, and removing a remaining portion of the first DFR layer.

Abstract: A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.

Abstract: A method of forming a photoresist pattern, capable of improving an adhesion property of the photoresist pattern formed on a substrate, includes forming a photocatalytic layer on a substrate, forming a negative-type photoresist layer on the photocatalytic layer, exposing the photoresist layer to ultraviolet rays, heat-treating the photoresist layer, and developing the photoresist layer to form the photoresist pattern. Thereby, applying the photocatalytic layer formed on various substrates, the photoresist pattern has excellent adhesion property and is capable of ensuring a high aspect ratio.