Abstract: Provided are a bi-functional compound that is positively charged at a first pH and negatively charged at a second pH, a solid support having the bi-functional compound immobilized thereon, and a method of isolating a nucleic acid, including: binding the bi-functional compound with a nucleic acid at a first pH and isolating the nucleic acid from the bi-functional compound at a second pH.

Abstract: Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

Abstract: Provided is a method comprising adding anions having a high charge density to a liquid medium; the liquid medium comprising molecules that hydrogen bond with one another; the anions interacting with the molecules of the liquid medium with a force that is stronger than the forces that produce hydrogen bonding between the molecules of the medium; and heating the liquid medium by irradiating it with microwaves.

Abstract: Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

Abstract: Provided is a pH dependent ion exchange material. The pH dependent ion exchange material has at least one monomer selected from the group consisting of M0, M1, M2 and M3 represented by the following formulae, provided that at least one monomer having A and at least one monomer having B are contained therein, the pH dependent ion exchange material having a degree of polymerization of 2-30,000. wherein A is a group —X(CH2)nY, wherein n is an integer from 1 to 10, X is a functional group which can react with an activated ester, and Y is a primary, secondary, tertiary amine or a nitrogen-containing aromatic heterocyclic base, B is a group —X?(CH2)nY?, wherein n is an integer from 1 to 10, X? is a functional group which can react with an activated ester, and Y? is an acid generating group having a pKa value of 4 or less, and R3 is an alkyl group having 1 to 10 carbon atoms.

Abstract: Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

Abstract: Provided is a Field-Effect Transistor (FET)-based biosensor including: a substrate; a source and a drain, disposed on the substrate, having opposite polarity to the substrate; a gate, disposed on the substrate, contacting the source and the drain; and an inorganic film capable of binding with a biomolecule, disposed on a surface of the gate. A method of manufacturing the FET-based biosensor and a method of detecting a biomolecule using the FET-based biosensor is also provided. The FET-based biosensor can be manufactured using a semiconductor fabrication process without performing an additional process. Therefore, the inorganic film can be selectively deposited on a surface of a specific gate of a single FET, or on the surfaces of some gates of a plurality of FETs using patterning. Furthermore, the FET-based biosensor can be used to effectively detect trace amounts of a target biomolecule in a sample.

Abstract: Provided is a method of storing a substrate having an active group or a probe molecule immobilized thereon. The method includes attaching a UV film to a surface of the substrate having the active group or probe immobilized thereon; and exposing the UV film to UV light, wherein the UV film comprises a UV-permeable base film and a pressure sensitive adhesive layer formed on a surface of the UV-permeable base film to immobilize the substrate, and when the pressure sensitive adhesive layer is exposed to the UV light, its adhesive force decreases.

Abstract: The present invention provides a method of manufacturing a solid support for biological analysis using a plastic material, the method including: depositing a metal film on a plastic substrate on which a microstructure is formed; depositing an inorganic oxide on the metal film; and anchoring a compound with an amino functional group or a compound with a water contact angle of 70 to 95 degrees on the inorganic oxide.

Abstract: Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

Abstract: Provided are a method of isolating a nucleic acid from a sample and a solid material for isolating the nucleic acid which can be used for the above method. The method includes contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first pH to bind the nucleic acid to the bifunctional material, the bifunctional material being positively charged at the first pH; and releasing the nucleic acid at a second pH which is higher than the first pH.

Abstract: A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.

Abstract: Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

Abstract: Provided are a method of isolating a nucleic acid from a sample and a solid material for isolating the nucleic acid which can be used for the above method. The method includes contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first pH to bind the nucleic acid to the bifunctional material, the bifunctional material being positively charged at the first pH; and releasing the nucleic acid at a second pH which is higher than the first pH.

Abstract: Provided are a pump unit and a centrifugal microfluidic system including the pump unit. The pump unit includes a gas generating agent. The gas generating agent includes a plurality of heating particles generating heat by absorbing energy and one of a sublimation material, azide, and azo compound mixed with the heating particles. When energy is supplied to the gas generating agent, the gas generating agent generates gas due to heat emitted from the heating particles to increase an air pressure around the gas generation agent so that the fluid is moved by the increased air pressure.

Abstract: A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.

Abstract: Provided are a method of producing a substrate having a patterned organosilane layer and a method of using the substrate having the patterned organosilane layer. The method of producing the substrate having the patterned organosilane layer, includes: coating organosilane on a substrate to obtain an organosilane layer; coating a photoresist material on the organosilane layer; exposing the photoresist material to light through a mask to obtain a patterned surface on the photoresist material; developing an exposed or unexposed region of the photoresist material using a developer; and wet etching a portion of the organosilane layer in the region from which the photoresist material has been removed, using a HF-containing solution as an etchant.

Abstract: Provided is a method of preparing a patterned spot microarray using a photocatalyst. The method comprises coating the photocatalyst on a substrate to form a photocatalyst layer, coating a composition comprising a functional group to be connected to a biomolecule on the photocatalyst layer to form an organic layer, spotting the biomolecule on the organic layer, positioning a photomask above a spot of the biomolecule; and irradiating the spot through the photomask to pattern the spot.

Abstract: The present invention relates to a method of isolating a nucleic acid from a microorganism cell. The method comprises contacting a nonplanar solid substrate with a cell-containing sample in a liquid medium having a pH of 3.0 to 6.0 so that the microorganism cell is bound to the nonplanar solid substrate, and lysing the microorganism cell bound to the nonplanar solid substrate. The invention also relates a device including the nonplanar solid substrate for isolating and amplifying a nucleic acid.

Abstract: Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic ion exchange material such that the sample reacts with the inorganic ion exchange material, and contacting the reacted sample with a means for capturing microorganisms.