Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.

Abstract: A method for controlling an electrostatically induced liquid spray includes the steps of: (1) generating a liquid spray from a liquid sample with an electrostatic spray nozzle device using an applied electric field, wherein at least a nozzle portion of the spray device is formed of an insulating material; (2) sensing a current of the liquid spray with a spray current sensing means placed in relation to the spray device; (3) comparing the sensed current of the liquid spray with a pre-selected current value, with a difference between the two representing a control signal; and (4) varying the applied electric field using a computer-controlled positioning mechanism that moves the spray device relative to an inlet of the object that receives the liquid spray and acts as a counter-electrode.

Abstract: The present invention provides a method of altering the function of TRPV1+ sensory afferent neurons in the pancreas as a way of treating, managing, alleviating, etc., the symptoms and/or underlying causes of diabetes or abnormal glucose metabolism by increasing the release of neuropeptides, such as substance P (sP) or other tachykinin peptide, in the pancreas. This may be achieved by injecting a TRPV1 agonist, such as a capsaicinoid compound or capsaicin analog, or a neuropeptide, such as sP or other tachykinin peptide, directly into the pancreas, or alternatively, by stimulating one or more intercostal and/or subcostal nerves of spinal nerves derived from one or more thoracic segments T8 through T12 by chemical, electrical, surgical, mechanical, etc., methods.

Abstract: Microfluidic nozzle array devices are provided with the body of each device having at least one nozzle extending outwardly from one surface of the body. The microfluidic nozzle array devices are fabricated using an injection molding process and find particular utility in a wide range of applications, including but not limited to nanospray/electrospray applications, mass spectrometer applications, optical spectrometry applications, spotting applications (i.e., DNA or protein array), etc. Manufactured nozzles can include sealed nozzle apexes which are subsequently opened by focusing energy from an energy source on the sealed apex resulting in the opening of the nozzle channel.

Abstract: An apparatus according to the present invention includes a plurality of sample spraying devices, each of which connects to an LC column. The apparatus includes an electrical circuit that can turn a high voltage between 1 and 5 KV on and off on the time-scale of nanoseconds to milliseconds. The circuit is controlled by a computer program which applies the high voltage to each spray device in a Hadamard sequence. The spray devices are positioned aiming at the mass spectrometer inlet. The preferred configuration of arrangements for the spray devices are in a circle or an arc of a circle around the inlet of a mass spectrometer.

Abstract: A process is disclosed for controlling inflammatory tissue access through release of neuropeptides, such as substance P (sP), to insulin-responsive sensory neurons, whereby simultaneous control of insulin sensitivity/resistance is manifested. In models of Type 1 and Type 2 diabetes, sensory afferents, in particular TRPV1, have fundamental roles in insulin/glucose homeostasis, islet physiology and autoimmune tissue inflammation. By manipulation of the TRPV1 neuro-?-cell circuit or enhancement of pancreatic sP levels, normalization of insulin resistance, clearance of inflammation and prevention of both Type 1 and Type 2 diabetes is realized.

Abstract: A process is disclosed for controlling inflammatory tissue access through release of neuropeptides, such as substance P (sP), to insulin-responsive sensory neurons, whereby simultaneous control of insulin sensitivity/resistance is manifested. In models of Type 1 and Type 2 diabetes, sensory afferents, in particular TRPV1, have fundamental roles in insulin/glucose homeostasis, islet physiology and autoimmune tissue inflammation. By manipulation of the TRPV1 neuro-?-cell circuit or enhancement of pancreatic sP levels, normalization of insulin resistance, clearance of inflammation and prevention of both Type 1 and Type 2 diabetes is realized.

Abstract: In accordance with one aspect of the present invention, a low-cost injection-molded plastic microfluidic device that performs the multiplexing of affinity or chromatographic sample cleanup and enrichment methods and the detection by nanospray mass spectrometry of analytes such as biomarkers in a single package to conserve sample, and processes body fluids with HPLC grade and even next-generation chromatographic resins such as nanoparticles to maximize the sensitivity of the detection.

Abstract: An approach is described for maintaining multiple presence objects associated with a single entity. The different presence objects correspond to different respective aspects of the entity's presence. Each presence object includes subscriber information and permission information associated therewith. The subscriber information identifies the subscribers that subscribe to receive notifications for a particular presence object. The permission information identifies the users who are entitled to receive notifications for the particular presence object. By virtue of the use of multiple objects, the entity can control access to its presence information with a high degree of granularity.

Abstract: An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.

Abstract: A method for controlling an electrostatically induced liquid spray includes the steps of: (1) generating a liquid spray from a liquid sample with an electrostatic spray nozzle device using an applied electric field, wherein at least a nozzle portion of the spray device is formed of an insulating material; (2) sensing a current of the liquid spray with a spray current sensing means placed in relation to the spray device; (3) comparing the sensed current of the liquid spray with a pre-selected current value, with a difference between the two representing a control signal; and (4) varying the applied electric field using a computer-controlled positioning mechanism that moves the spray device relative to an inlet of the object that receives the liquid spray and acts as a counter-electrode.

Abstract: A system for controlling an electrostatically induced liquid spray includes an electrostatic spray device for generating a liquid spray from a liquid sample; a spray current sensing means placed in relation to the spray device and configured to generate a current output signal that represents a current of the liquid spray; and a mechanism that receives the current output signal and compares it to a pre-selected current value, with a difference between the two representing a control signal that is sent to one of (1) a pump that regulates the flow rate of the liquid sample and (2) a power supply to regulate an electric field associated with the spray device that generates the liquid spray according to a set level of current.

Abstract: A process is disclosed for controlling inflammatory tissue access through release of neuropeptides, such as substance P (sP), to insulin-responsive sensory neurons, whereby simultaneous control of insulin sensitivity/resistance is manifested. In models of Type 1 and Type 2 diabetes, sensory afferents, in particular TRPV1, have fundamental roles in insulin/glucose homeostasis, islet physiology and autoimmune tissue inflammation. By manipulation of the TRPV1 neuro-?-cell circuit or enhancement of pancreatic sP levels, normalization of insulin resistance, clearance of inflammation and prevention of both Type 1 and Type 2 diabetes is realized.

Abstract: An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.

Abstract: An electronic license plate system capable of changing information displayed thereon and method for the same is a display device installed on in a motor vehicle showing information according to random instructions given by a traffic control system, serving as an illuminated license plate for easy visual identification. The displayed contents of the license plate varies with time and location to prevent counterfeiting or a shut-down event, and alertness and road security may be raised in areas with poor visibility due to the illuminated license plate. The electronic license plate working with the traffic control system may reach the functions of unmanned charge, dynamic geographic information, unmanned traffic control, road use information, and traffic statistics, and may provide a functional intelligent traffic system (ITS) for prevention of license plate counterfeiting and vehicle theft.

Abstract: A polymer-based microfluidic device and its manufacturing methods. This microfluidic device is suitable for chromatographic and electrophoretic separations in which the detection of the components in the fluid is by means of ultraviolet (UV) spectroscopy. The device further comprises at least one cooling channel adjacent the separation channel, said cooling channel conducting a cooling fluid through the device for removing heat from the the separation channel. At least one section of the separation channel has an increased cross-sectional dimension with respect to other sections of said channel to provide an increased optical path length when the device is used in an ultraviolet detection arrangement.

Abstract: A microfluidic device is provided and comprises a device that is assembled from segments of microfluidic features using built-in alignment features, such as the raised walls of the channels or protrusion/recess pairs to comprise channels, nozzles, optical detectors, distillation columns, etc., to form a device for a specific application, such as an electrospray-mass spectrometer interface. Some of the segments can be of standard dimensions with standard alignment features so that the user may assemble microfluidic devices for specific applications using standard prefabricated segments as part thereof.