Patents by Inventor Lars Beier
Lars Beier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130071913Abstract: The present invention relates to the use of alpha-amylase variants having improved activity relative to the parent enzyme at low temperature, including improved washing and/or dishwashing performance and/or increased stability at low temperature. The invention further relates a method for doing laundry, dish wash and/or cleaning such as institutional cleaning and to compositions for use at low temperature.Type: ApplicationFiled: December 22, 2010Publication date: March 21, 2013Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Svend Kaasgaard, Lars Beier, Jens Obro
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Patent number: 8361526Abstract: Dough with a high sucrose content (such as cake dough) tends to inhibit the activity of an anti-staling amylase such as Novamyl®, making it less effective to prevent the staling of dough-based products with high sucrose content such as cakes. A good anti-staling effect in cakes can be achieved by using a carefully selected anti-staling amylase with certain properties. Analysis of a 3D structure of Novamyl® shows that sucrose may inhibit by binding in the active site. Sucrose docks into the active site of Novamyl® differently from the substrate or inhibitor in published models 1QHO and 1QHP. This finding is used to design sucrose-tolerant variants.Type: GrantFiled: June 5, 2008Date of Patent: January 29, 2013Assignee: Novozymes A/SInventors: Lars Beier, Esben Peter Friis, Henrik Lundqvist, Peter Kamp Hansen, Tina Spendler
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Publication number: 20130000055Abstract: The present invention relates to cleaning compositions comprising variants of an alpha-amylase and methods of treating surfaces such as textiles with aqueous liquor comprising such compositions, especially at low temperatures.Type: ApplicationFiled: June 29, 2012Publication date: January 3, 2013Inventors: Michelle Jackson, Philip Frank Souter, Lindsay Suzanne Bewick, Svend Kaasgaard, Jens Oebro, Signe Eskildsen Larsen, Allan Svendsen, Annette Helle Johansen, Michael Skjoet, Carsten Andersen, Lars Beier, Esben Peter Friis, Miguel Duarte Guilherme Pereira Toscano, Mads Bjoernvad, Frank Winther Rasmussen, Liv Spaangner Christiansen
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Publication number: 20120288585Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 9, 2010Publication date: November 15, 2012Applicant: NOVOZYMES A/SInventors: Lars Beier, Miguel Duarte Toscano, Esben Peter Friis, Merete Moeller Engelsen, Henrik Lundkvist, Sune Sauer Lobedanz
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Patent number: 8309337Abstract: The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability. The inventors developed a method to identify and alleviate such steric hindrance in the resulting polypeptides. In an alignment of the three-dimensional structures, steric hindrance is indicated when residues from two different structures are located within a certain distance.Type: GrantFiled: August 3, 2011Date of Patent: November 13, 2012Assignee: Novozymes A/SInventors: Allan Svendesn, Lars Beier
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Publication number: 20120270253Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: ApplicationFiled: June 26, 2012Publication date: October 25, 2012Applicant: NOVOZYMES A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Publication number: 20120225164Abstract: A method of mashing comprising providing a grist comprising malt and adjunct; and contacting the grist with a pullulanase; an alpha amylase; and a maltogenic alpha amylase and/or a beta amylase to make a wort. An enzyme composition comprising a pullulanase; an alpha amylase; and a maltogenic alpha amylase and/or a beta amylase and the use of these enzymes in brewing is disclosed.Type: ApplicationFiled: November 11, 2010Publication date: September 6, 2012Applicant: NOVOZYMES A/SInventors: Anne Mette Bhatia Frederiksen, Lars Beier, Stefan Kreisz
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Patent number: 8232086Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: January 20, 2011Date of Patent: July 31, 2012Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Publication number: 20120172280Abstract: The present invention relates to protease variants. The present invention also relates to polynucleotides encoding the variant protease variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes, such as, in laundry and detergent compositions.Type: ApplicationFiled: September 24, 2010Publication date: July 5, 2012Applicant: NOVOZYMES A/SInventors: Jurgen Carsten Franz Knotzel, Maria Norman Hockauf, Lars Beier, Astrid Benie
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Publication number: 20120107873Abstract: The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.Type: ApplicationFiled: August 5, 2010Publication date: May 3, 2012Applicant: NOVOZYMES A/SInventors: Jesper Vind, Jeppe Wegener Tams, Lars Beier, Carsten Lillelund Olsen
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Publication number: 20110305795Abstract: Dough with a high sucrose content (such as cake dough) tends to inhibit the activity of an anti-staling amylase such as Novamyl, making it less effective to prevent the staling of dough-based products with high sucrose content such as cakes. A good anti-staling effect in cakes can be achieved by using a carefully selected anti-staling amylase with certain properties. Analysis of a 3D structure of Novamyl shows that sucrose may inhibit by binding in the active site. Sucrose docks into the active site of Novamyl differently from the substrate or inhibitor in published models 1QHO and 1QHP. This finding is used to design sucrose-tolerant variants.Type: ApplicationFiled: December 9, 2010Publication date: December 15, 2011Applicant: Novozymes A/SInventors: Lars Beier, Esben Peter Friis, Henrik Lundquist
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Publication number: 20110287142Abstract: The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability. The inventors developed a method to identify and alleviate such steric hindrance in the resulting polypeptides. In an alignment of the three-dimensional structures, steric hindrance is indicated when residues from two different structures are located within a certain distance.Type: ApplicationFiled: August 3, 2011Publication date: November 24, 2011Applicant: Novozymes A/SInventors: Allan Svendsen, Lars Beier
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Patent number: 8017371Abstract: The inventors realized that the diversity generated by conventional methods may be limited by steric hindrance between amino acid residues in the three-dimensional structures of the resulting polypeptides. The steric hindrance may occur between amino acid residues at widely different positions in the amino acid sequences, e.g. between residues in two different domains of the 3D structure, and resulting polypeptides which include such steric hindrance may never be observed in the conventional recombination methods because they may be expressed in poor yields or may have poor activity or stability. The inventors developed a method to identify and alleviate such steric hindrance in the resulting polypeptides. In an alignment of the three-dimensional structures, steric hindrance is indicated when residues from two different structures are located within a certain distance.Type: GrantFiled: February 11, 2010Date of Patent: September 13, 2011Assignee: Novozymes A/SInventors: Allan Svendesn, Lars Beier
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Publication number: 20110212876Abstract: The present invention relates to cleaning compositions comprising variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme and cleaning processes comprising such compositions.Type: ApplicationFiled: February 10, 2011Publication date: September 1, 2011Inventors: Michelle MEEK, Philip Frank Souter, Lindsay Suzanne Bewick, Allan Svendsen, Annette Helle Johansen, Mads Eskelund Bjoernvad, Frank Winther Rasmussen, Michael Skjoet, Signe Eskildsen Larsen, Jens Oebro, Svend Kaasgaard, Lars Beier
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Publication number: 20110195481Abstract: The present invention relates to variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme, compositions comprising the variants, nucleic acids encoding the variants, methods of producing the variants, and methods for using the variants.Type: ApplicationFiled: February 10, 2011Publication date: August 11, 2011Applicant: Novozymes A/SInventors: Allan Svendsen, Annette Helle Johansen, Mads Bjoernvad, Frank W. Rasmussen, Michael Skjoet, Signe Eskildsen Larsen, Jens Oebro, Svend Kaasgaard, Lars Beier
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Publication number: 20110159570Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: ApplicationFiled: January 20, 2011Publication date: June 30, 2011Applicant: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Patent number: 7908094Abstract: The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.Type: GrantFiled: March 30, 2009Date of Patent: March 15, 2011Assignee: Novozymes A/SInventors: Joel Cherry, Allan Svendsen, Carsten Andersen, Lars Beier, Torben Peter Frandsen
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Patent number: 7892806Abstract: The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.Type: GrantFiled: February 2, 2010Date of Patent: February 22, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Lars Beier, Jesper Vind, Tina Spendler, Morten Tovborg Jensen
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Publication number: 20100322915Abstract: The invention relates to novel variants of a protease derived from Nocardiopsis sp. (SEQ ID NO: 1) and closely related proteases, as well as their pharmaceutical use. The variants show improved performance in the treatment of pancreatic exocrine insufficiency (PEI). The variants may be combined with a lipase and/or an amylase. Other examples of medical indications are: Treatment of digestive disorders, pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II.Type: ApplicationFiled: December 2, 2008Publication date: December 23, 2010Applicants: Novozymes A/S, Solvay Pharmaceuticals, GmbHInventors: Allan Svendsen, Lars Beier, Signe Eskildsen Larsen, Thomas Lenhard, Tanja Maria Rosenkilde Kjaer, Peter Colin Gregory
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Publication number: 20100215802Abstract: Dough with a high sucrose content (such as cake dough) tends to inhibit the activity of an anti-staling amylase such as Novamyl®, making it less effective to prevent the staling of dough-based products with high sucrose content such as cakes. A good anti-staling effect in cakes can be achieved by using a carefully selected anti-staling amylase with certain properties. Analysis of a 3D structure of Novamyl® shows that sucrose may inhibit by binding in the active site. Sucrose docks into the active site of Novamyl® differently from the substrate or inhibitor in published models 1QHO and 1QHP. This finding is used to design sucrose-tolerant variants.Type: ApplicationFiled: June 5, 2008Publication date: August 26, 2010Applicant: Novozymes A/SInventors: Lars Beier, Peter Esben Friis, Henrik Lundqvist, Peter Kamp Hansen, Tina Spendler