Abstract: The invention provides topical formulations and methods comprising charged bioactive agents complexed with amphipol polymers for dermal and transdermal delivery, optionally further including TJ-modulating peptides.

Abstract: The invention provides topical formulations and methods comprising charged bioactive agents complexed with amphipol polymers for dermal and transdermal delivery, optionally further including TJ-modulating peptides.

Abstract: Improved chemodenervation agents are provided comprising polypeptide ?-neurotoxins having high binding specificity and selectivity for the human muscular ?1 nAChR instead of the human neuronal ?7 nAChR, along with pharmaceutical compositions and methods of use.

Abstract: The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing.

Abstract: The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.

Abstract: Improved chemodenervation agents are provided comprising polypeptide ?-neurotoxins having high binding specificity and selectivity for the human muscular ?1 nAChR instead of the human neuronal ?7 nAChR, along with pharmaceutical compositions and methods of use.

Abstract: Methods and compositions are provided for enhanced specificity and sensitivity of amplification reaction mixtures. Compositions in accordance with the present invention provide for reduced formation of primer-aggregates during amplification reactions. Reaction mixes include dNTPs, where a portion of the dNTPs has been replaced with an unconventional nucleotide, e.g., dUTP. Unconventional nucleotide concentrations are typically between 10% to 50% of the concentration of one of the standard dNTP. In some compositions the unconventional nucleotide is dUTP which replaces from about 10% to about 50% of the dTTP in the dNTP mix.

Abstract: The invention relates to an active enzyme of bacterial, fungal, viral, or archae origin, wherein the enzyme is coupled, preferably covalently coupled with at least one polymer having a molecular weight between about 500 to about 20,000 daltons selected from the group consisting of polyethylene glycol and polypropylene glycol. The invention relates in particular to uses thereof of such as in molecular biology as well as kits comprising such enzymes. In preferred embodiments the enzymes of the invention are nucleic acid modifying or replicating enzymes.

Abstract: The invention relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention also relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention further relates to a method for enzymatic nucleic acid synthesis comprising the steps of, (a) providing in a reaction mixture, a polymerase activity, a nucleic acid template, a zwitterionic detergent, a buffer, a salt, nucleotides and an inert protein and, (b) incubating the reaction mixture at a temperature which enables nucleic acid synthesis.

Abstract: Methods and compositions are provided for enhanced signal intensity and storage stability of standard nucleic acid amplification buffers, real-time PCR buffers or both. Buffers in accordance with the present invention include anti-freeze protein(s) (AFPs), optionally with a carrier protein, such as BSA.

Abstract: The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.

Abstract: The invention provides Pol III ? mutants, and modified Pol III replicases comprising the same, and methods of using modified Pol III replicases for a variety of nucleic acid replication reactions.

Abstract: The present invention relates to a detection and quantification method of different sets of target molecules (1, 3), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface (6) and comprising different sets (A, B) of capture molecules (2, 4), present in different locations (8, 9) of the solid support surface (6).

Abstract: Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

Abstract: Methods and compositions are provided for enhanced specificity and sensitivity of amplification reaction mixtures. Compositions in accordance with the present invention provide for reduced formation of primer-aggregates during amplification reactions. Reaction mixes include dNTPs, where a portion of the dNTPs has been replaced with an unconventional nucleotide, e.g., dUTP. Unconventional nucleotide concentrations are typically between 10% to 50% of the concentration of one of the standard dNTP. In some compositions the unconventional nucleotide is dUTP which replaces from about 10% to about 50% of the dTTP in the dNTP mix.

Abstract: The invention provides Pol III ? mutants, and modified Pol III replicases comprising the same, and methods of using modified Pol III replicases for a variety of nucleic acid replication reactions.

Abstract: The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing.

Abstract: Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

Abstract: The present invention relates to a detection and quantification method of different sets of target molecules (1, 3), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface (6) and comprising different sets (A, B) of capture molecules (2, 4), present in different locations (8, 9) of the solid support surface (6).

Abstract: Compositions and methods are provided for the in vitro amplification of nucleic acid and, in particular, for the amplification of extrachromosomal nucleic acid molecules having a molecular weight of one kilobase or greater. The design and use of primers, low ionic strength amplification buffer, low co-solvent containing buffer, and combined polymerization/ligation reaction buffers are described in this regard.